Sheep anti Human C4d antibody (2222-7054) recognizes human C3d and gave a single arc in the alpha region when tested by IEP against aged human plasma (C3d) and a continuous double arc in the alpha and beta regions when tested against fresh human plasma (C3 and C3d). Identity was confirmed by double diffusion (ouchterlony) versus human plasma and a known anti-human C3d.
- Target Species
- Product Form
- Ig fraction - liquid
- Ig fraction prepared by ion exchange chromatography
- Buffer Solution
- Glycine buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 0.1% EACA 0.01% Benzamidine 1 mM EDTA
- Purified C3d from pooled activated serum.
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- P01024 Related reagents
- Entrez Gene
- C3 Related reagents
- GO Terms
- GO:0005102 receptor binding
- GO:0004866 endopeptidase inhibitor activity
- GO:0005615 extracellular space
- GO:0006954 inflammatory response
- GO:0006957 complement activation, alternative pathway
- GO:0006958 complement activation, classical pathway
- GO:0007186 G-protein coupled receptor protein signaling pathway
- GO:0010575 positive regulation vascular endothelial growth factor production
- For research purposes only
Applications of C3d antibody
|Application Name||Verified||Min Dilution||Max Dilution|
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
|Description||Product Code||Pack Size||Applications||List Price||Quantity|
|Rabbit anti Sheep IgG (H/L):Biotin||5184-2304||1.5 ml||C E F WB|
|Donkey anti Sheep/Goat IgG:DyLight®488||STAR88D488GA||0.1 mg||F IF|
|Donkey anti Sheep/Goat IgG:DyLight®549||STAR88D549GA||0.1 mg||F IF|
|Donkey anti Sheep/Goat IgG:DyLight®649||STAR88D649GA||0.1 mg||F IF|
|Donkey anti Sheep/Goat IgG:FITC||STAR88F||1 mg||C|
|Donkey anti Sheep/Goat IgG:HRP||STAR88P||1 ml||C E P WB|
Product Specific References
References for C3d antibody
Luo, Y.H. et al. (2007) Streptococcal pyrogenic exotoxin B antibodies in a mouse model of glomerulonephritis.
Kidney Int. 72: 716-24.