C3c antibody

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Sheep anti Human C3c:FITC

Sheep anti Human C3c:HRP

Sheep anti Human C3c

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG

Product Code	Applications	Pack Size
2222-6604F	E IF	1 mg
2222-6604P	E WB	1 ml
2222-6604	C E	1 ml

Sheep anti Human C3c antibody detects human complement 3c, a polypeptide fragment which is present in C3. C3 plays a central role in the activation of the complement system. Processing of C3 by C3 convertase is the central reaction in both classical and alternative complement pathways, resulting in C3a and C3b. C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates, and help to initiate the complement cascade, potentially resulting in cellular apoptosis. C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.

C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c and C3dg. Defects in C3 can result in susceptibility to pyogenic infection.

This antbody shows minimal reactivity with related serum proteins.

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Preparation: Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.01% Thiomersal
HRP Stabiliser (BUF052A)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Immunogen: Native human C3c purified from serum
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Approx. Protein Concentrations: IgG concentration 5.0 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01024 Related reagents
Entrez Gene: C3 Related reagents
GO Terms: GO:0005102 receptor binding; GO:0004866 endopeptidase inhibitor activity; GO:0005615 extracellular space; GO:0006954 inflammatory response; GO:0006957 complement activation, alternative pathway; GO:0006958 complement activation, classical pathway; GO:0007186 G-protein coupled receptor protein signaling pathway; GO:0010575 positive regulation vascular endothelial growth factor production
Regulatory: For research purposes only

Applications of C3c antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA	1/500	1/50K
Immunofluorescence	1/10	1/50
ELISA	1/200	1/800
Western Blotting	1/500	1/2000
ELISA	1/400
Immunohistology - Frozen	1/50

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Rabbit anti Sheep IgG (H/L):Biotin	5184-2304	1.5 ml	C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488	STAR88D488GA	0.1 mg	F IF
Donkey anti Sheep/Goat IgG:DyLight®549	STAR88D549GA	0.1 mg	F IF
Donkey anti Sheep/Goat IgG:DyLight®649	STAR88D649GA	0.1 mg	F IF
Donkey anti Sheep/Goat IgG:FITC	STAR88F	1 mg	C
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB

Useful Reagents Available

Description	Product Code	Pack Size	Applications
AbGUARD® HRP Stabilizer Plus	BUF052A	100 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052B	500 ml	C E P WB
AbGUARD® HRP Stabilizer Plus	BUF052C	1000 ml	C E P WB
TMB Signal+	BUF054A	100 ml	E
TMB Core	BUF056A	100 ml	E
TMB Core+	BUF062A	100 ml	E

Product Specific References

References for C3c antibody

Janssen, B.J. et al. (2005) Structures of complement component C3 provide insights into the function and evolution of immunity.
Nature. 437 (7058): 505-11.

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