APEX1 antibody
Goat anti APEX1
APEX1 antibody recognizes DNA-(apurinic or apyrimidinic site) lyase, also known as APEX nuclease, APEN Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1 or Redox factor-1. The antibody has been extensively validated for western blotting using whole cell lysates.
APEX1 Antibody with Control Lysate
APEX1 antibody recognizes DNA-(apurinic or apyrimidinic site) lyase, also known as APEX nuclease, APEN Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1 or Redox factor-1. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00060 | WB | 100 µl | ||||
| VPA00060KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human APEX1 antibody recognizes apurinic/apyrimidinic endonuclease 1 (APE1), also known as redox effector factor-1 (Ref-1). APEX1 is a 35 kDa multifunctional protein that is ubiquitously expressed at high basal levels.
APEX1 acts on apurinic/apyrimidinic (AP) sites in DNA as a major member of the base excision repair (BER) pathway and is involved in oxidative DNA damage repair. It is responsible for the maintenance of the cellular proliferative rate, and acts by modulating DNA binding activity of several transcription factors including NFkB, Egr-1, p53, AP-1, CREB, HIF-alpha and members of the Pax family. APEX1 has been shown to regulate hsp90 expression in thyroid cancer cells, in a novel tumorigenic pathway. APEX1 is also required in normal embryonic hematopoiesis.
Goat anti Human APEX1 antibody detects APEX1 as a single band of 35 kDa in a wide range of human cell line lysates tested.
APEX1 acts on apurinic/apyrimidinic (AP) sites in DNA as a major member of the base excision repair (BER) pathway and is involved in oxidative DNA damage repair. It is responsible for the maintenance of the cellular proliferative rate, and acts by modulating DNA binding activity of several transcription factors including NFkB, Egr-1, p53, AP-1, CREB, HIF-alpha and members of the Pax family. APEX1 has been shown to regulate hsp90 expression in thyroid cancer cells, in a novel tumorigenic pathway. APEX1 is also required in normal embryonic hematopoiesis.
Goat anti Human APEX1 antibody detects APEX1 as a single band of 35 kDa in a wide range of human cell line lysates tested.
Product Details
- Target Species
- Human
- Western Blotting
- Goat anti APEX1 detects a band of approximately 35 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin - Immunogen
- Peptide with sequence PKRGKKGAVAEDGD-C from the N terminus of the protein sequence.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P27695 Related reagents
- Entrez Gene
- APEX1 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0003684 damaged DNA binding
- GO:0003713 transcription coactivator activity
- GO:0003714 transcription corepressor activity
- GO:0003723 RNA binding
- GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
- GO:0005739 mitochondrion
- GO:0004523 ribonuclease H activity
- GO:0004528 phosphodiesterase I activity
- GO:0004844 uracil DNA N-glycosylase activity
- GO:0005783 endoplasmic reticulum
- GO:0046872 metal ion binding
- GO:0005840 ribosome
- GO:0005730 nucleolus
- GO:0005813 centrosome
- GO:0016491 oxidoreductase activity
- GO:0006284 base-excision repair
- GO:0006310 DNA recombination
- GO:0008408 3'-5' exonuclease activity
- GO:0016607 nuclear speck
- GO:0016890 site-specific endodeoxyribonuclease activity, specific for altered base
- GO:0031490 chromatin DNA binding
- GO:0043488 regulation of mRNA stability
- GO:0045739 positive regulation of DNA repair
- GO:0045768 positive regulation of anti-apoptosis
- GO:0048471 perinuclear region of cytoplasm
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of APEX1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
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| Description | Product Code | Pack Size | Applications | List Price | Quantity |
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| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
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