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APEX1 antibody

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KO Validated
Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 1 Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 2 Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) thumbnail image 3
Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with APEX1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 22 second exposure. Arrow points to APEX1 (molecular weight 35 kDa)
Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 2
Western blot analysis of APEX1 CRISPR knockout HeLa (APEX1 KO) and wild type HeLa (WT) whole cell lysates probed with Goat anti APEX1 antibody (VPA00060) followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (Fc) (1/10,000 STAR88P) and hFAB rhodamine anti-tubulin primary antibody (12004165) and visualized on the ChemiDoc MP with an 100 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.
Anti APEX1 Antibody (PrecisionAb Polyclonal Antibody) gallery image 3
Normalized signal intensity of APEX1 from CRISPR knockout and wild type lysates. Signal intensity was normalized to the tubulin signal.

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WB

Goat anti APEX1

Product Type
PrecisionAb Polyclonal
Isotype
Polyclonal IgG
Format
Purified
Specificity
APEX1

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VPA00060
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl
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Goat anti Human APEX1 antibody recognizes apurinic/apyrimidinic endonuclease 1 (APE1), also known as redox effector factor-1 (Ref-1). APEX1 is a 35 kDa multifunctional protein that is ubiquitously expressed at high basal levels.

APEX1 acts on apurinic/apyrimidinic (AP) sites in DNA as a major member of the base excision repair (BER) pathway and is involved in oxidative DNA damage repair. It is responsible for the maintenance of the cellular proliferative rate, and acts by modulating DNA binding activity of several transcription factors including NFkB, Egr-1, p53, AP-1, CREB, HIF-alpha and members of the Pax family. APEX1 has been shown to regulate hsp90 expression in thyroid cancer cells, in a novel tumorigenic pathway. APEX1 is also required in normal embryonic hematopoiesis.

Goat anti Human APEX1 antibody detects APEX1 as a single band of 35 kDa in a wide range of human cell line lysates tested.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Goat anti APEX1 detects a band of approximately 35 kDa in Jurkat cell lysates.
Product Form
Purified IgG - liquid
Preparation
Goat polyclonal antibody purified by affinity chromatography.
Buffer Solution
TRIS buffered saline.
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
0.5% Bovine Serum Albumin
Immunogen
Peptide with sequence PKRGKKGAVAEDGD-C from the N terminus of the protein sequence.
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Donkey anti Sheep/Goat IgG:HRP STAR88P C E P WB 1 ml loader
List Price Your Price
loader
Description Donkey anti Sheep/Goat IgG:HRP

UniProt
P27695
Entrez Gene
APEX1
GO Terms
GO:0005515 protein binding
GO:0003684 damaged DNA binding
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0003723 RNA binding
GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity
GO:0005739 mitochondrion
GO:0004523 ribonuclease H activity
GO:0004528 phosphodiesterase I activity
GO:0004844 uracil DNA N-glycosylase activity
GO:0005783 endoplasmic reticulum
GO:0046872 metal ion binding
GO:0005840 ribosome
GO:0005730 nucleolus
GO:0005813 centrosome
GO:0016491 oxidoreductase activity
GO:0006284 base-excision repair
GO:0006310 DNA recombination
GO:0008408 3'-5' exonuclease activity
GO:0016607 nuclear speck
GO:0016890 site-specific endodeoxyribonuclease activity, specific for altered base
GO:0031490 chromatin DNA binding
GO:0043488 regulation of mRNA stability
GO:0045739 positive regulation of DNA repair
GO:0045768 positive regulation of anti-apoptosis
GO:0048471 perinuclear region of cytoplasm
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