APEX1 antibody

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Goat anti APEX1

APEX1 antibody recognizes DNA-(apurinic or apyrimidinic site) lyase, also known as APEX nuclease, APEN Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1 or Redox factor-1. The antibody has been extensively validated for western blotting using whole cell lysates.

APEX1 Antibody with Control Lysate

Product Type: PrecisionAb™ Polyclonal
Isotype: Polyclonal IgG
Format: Purified

Product Code	Applications	Pack Size
VPA00060	WB	100 µl
VPA00060KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Goat anti Human APEX1 antibody recognizes apurinic/apyrimidinic endonuclease 1 (APE1), also known as redox effector factor-1 (Ref-1). APEX1 is a 35 kDa multifunctional protein that is ubiquitously expressed at high basal levels.

APEX1 acts on apurinic/apyrimidinic (AP) sites in DNA as a major member of the base excision repair (BER) pathway and is involved in oxidative DNA damage repair. It is responsible for the maintenance of the cellular proliferative rate, and acts by modulating DNA binding activity of several transcription factors including NFkB, Egr-1, p53, AP-1, CREB, HIF-alpha and members of the Pax family. APEX1 has been shown to regulate hsp90 expression in thyroid cancer cells, in a novel tumorigenic pathway. APEX1 is also required in normal embryonic hematopoiesis.

Goat anti Human APEX1 antibody detects APEX1 as a single band of 35 kDa in a wide range of human cell line lysates tested.

Product Details

Target Species: Human
Western Blotting: Goat anti APEX1 detects a band of approximately 35 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Goat polyclonal antibody purified by affinity chromatography
Preparation: 20μl Goat polyclonal antibody purified by affinity chromatography
Buffer Solution: TRIS buffered saline
Buffer Solution: TRIS buffered saline
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Preservative Stabilisers: 0.02% Sodium Azide (NaN₃)
0.5% Bovine Serum Albumin
Immunogen: Peptide with sequence PKRGKKGAVAEDGD-C from the N terminus of the protein sequence.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg Jurkat lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.

More Information

UniProt: P27695 Related reagents
Entrez Gene: APEX1 Related reagents
GO Terms: GO:0005515 protein binding; GO:0003684 damaged DNA binding; GO:0003713 transcription coactivator activity; GO:0003714 transcription corepressor activity; GO:0003723 RNA binding; GO:0003906 DNA-(apurinic or apyrimidinic site) lyase activity; GO:0005739 mitochondrion; GO:0004523 ribonuclease H activity; GO:0004528 phosphodiesterase I activity; GO:0004844 uracil DNA N-glycosylase activity; GO:0005783 endoplasmic reticulum; GO:0046872 metal ion binding; GO:0005840 ribosome; GO:0005730 nucleolus; GO:0005813 centrosome; GO:0016491 oxidoreductase activity; GO:0006284 base-excision repair; GO:0006310 DNA recombination; GO:0008408 3'-5' exonuclease activity; GO:0016607 nuclear speck; GO:0016890 site-specific endodeoxyribonuclease activity, specific for altered base; GO:0031490 chromatin DNA binding; GO:0043488 regulation of mRNA stability; GO:0045739 positive regulation of DNA repair; GO:0045768 positive regulation of anti-apoptosis; GO:0048471 perinuclear region of cytoplasm
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of APEX1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000
Western Blotting			1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB
Donkey anti Sheep/Goat IgG:HRP	STAR88P	1 ml	C E P WB

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