Alpha 2 Macroglobulin antibody

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Sheep anti Alpha 2 Macroglobulin

alpha-2-Macroglobulin antibody recognizes alpha-2-macroglobulin, also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 or alpha-2-M. This antibody has been extensively validated for western blotting using whole cell lysates.

Alpha 2 Macroglobulin Antibody with Control Lysate

alpha-2-Macroglobulin antibody recognizes alpha-2-macroglobulin, also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 or alpha-2-M. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Polyclonal
Isotype
Polyclonal IgG
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VPA00200 WB 100 µl
VPA00200KT WB 2 Westerns
With Lysate 400µg
Sheep anti Human alpha 2 macroglobulin antibody recognizes the alpha 2 macroglobulin, also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5 and alpha-2-M.

Alpha 2 macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. Alpha 2 macroglobulin is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits (provided by RefSeq, Jul 2008).

Sheep anti Human alpha 2 macroglobulin antibody detects a band of 180 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti alpha 2 macroglobulin detects a band of approximately 180 kDa in HepG2 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Sheep polyclonal antibody purified by affinity chromatography on Protein G
Preparation
20μl Sheep polyclonal antibody purified by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified human alpha 2 macroglobulin
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HepG2 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P01023 Related reagents
Entrez Gene
A2M Related reagents
GO Terms
GO:0001869 negative regulation of complement activation, lectin pathway
GO:0002576 platelet degranulation
GO:0005829 cytosol
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005096 GTPase activator activity
GO:0005615 extracellular space
GO:0007264 small GTPase mediated signal transduction
GO:0007597 blood coagulation, intrinsic pathway
GO:0019899 enzyme binding
GO:0019959 interleukin-8 binding
GO:0019966 interleukin-1 binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0043120 tumor necrosis factor binding
GO:0051056 regulation of small GTPase mediated signal transduction
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of Alpha 2 Macroglobulin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):HRP 5184-2504 2 mg C E WB
Rabbit anti Sheep IgG (H/L):HRP 5184-2504 2 mg C E WB

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