ALOX15 antibody
Goat anti ALOX15
ALOX15 antibody recognizes arachidonate 15-lipoxygenase, also known as 12/15-lipoxygenase, 15-LOX, 15-lipooxygenase-1, leukocyte-type or arachidonate omega-6 lipoxygenase. This antibody has been extensively validated for western blotting using whole cell lysates.
ALOX15 Antibody with Control Lysate
ALOX15 antibody recognizes arachidonate 15-lipoxygenase, also known as 12/15-lipoxygenase, 15-LOX, 15-lipooxygenase-1, leukocyte-type or arachidonate omega-6 lipoxygenase. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Polyclonal
- Isotype
- Polyclonal IgG
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VPA00127 | WB | 100 µl | ||||
| VPA00127KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Goat anti Human ALOX15 antibody recognizes the Arachidonate 15-lipoxygenase, also known as 15-LOX , 15-LOX-1, 12/15-lipoxygenase, leukocyte-type Arachidonate 12-lipoxygenase, 12-LOX or Arachidonate omega-6 lipoxygenase.
Goat anti Human ALOX15 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Goat anti Human ALOX15 antibody detects a band of 70 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ALOX15 detects a band of approximately 70 kDa in HEK293 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Goat polyclonal antibody purified by affinity chromatography.
- Preparation
- 20μl Goat polyclonal antibody purified by affinity chromatography
- Buffer Solution
- TRIS buffered saline
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5% BSA
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3) 0.5% BSA
- Immunogen
- Peptide with the sequence C-HYKTDVAVKDDPE, from the internal region of the protein sequence
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg HEK293 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
More Information
- UniProt
- P16050 Related reagents
- Entrez Gene
- ALOX15 Related reagents
- GO Terms
- GO:0005506 iron ion binding
- GO:0005634 nucleus
- GO:0055114 oxidation-reduction process
- GO:0006954 inflammatory response
- GO:0016165 lipoxygenase activity
- GO:0019370 leukotriene biosynthetic process
- GO:0050473 arachidonate 15-lipoxygenase activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ALOX15 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
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