Alkaline Phosphatase antibody
Sheep anti Human alkaline phosphatase antibody used for the detection of alkaline phosphatase in Caco-2/TC7 cells by western blotting.
Image caption:
PPM supply induces movement of SR-BI/CLA-1 towards raft microdomains. (A) Caco-2/TC7 cells were harvested in the presence of Triton X-100 and the lysate fractionated on a 5–40% sucrose gradient. Eleven fractions were collected for immunoblots of SR-BI/CLA-1, EEA1 (early endosome antigen 1) and flottilin-1 (raft marker). (B) Caco-2/TC7 cells were cultured in the absence (control) or presence of PPM or IPM for 10 min and then harvested in the presence of Triton X-100. Cell lysates were applied to a 5–40% sucrose gradient and eleven fractions collected. Fractions 3 to 8 were analyzed by immunoblotting with antibodies against SR-BI/CLA-1 (left panel) and flottilin-1 (right panel). (C) Immunolocalization of SR-BI/CLA-1 and alkaline phosphatase (PLAP, used as raft marker) in the brush border of Caco-2/TC7 cells supplied with PPM. SR-BI/CLA-1 is labelled with anti-rabbit immunoglobulin-gold complexes (18-nm particles) and PLAP with anti-sheep immunoglobulin-gold complexes (12-nm particles). MV, microvilli; bar, 100 nm.
From: Béaslas O, Cueille C, Delers F, Chateau D, Chambaz J, Rousset M, et al. (2009)
Sensing of Dietary Lipids by Enterocytes: A New Role for SR-BI/CLA-1.
PLoS ONE 4(1): e4278.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Sheep anti Human alkaline phosphatase antibody used for the evaluation of alkaline phosphatase expression in human muscle derived cells (mdcs) by flow cytometry.
Image caption:
Characterization and phenotype of human mdcs. a. Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. Scale bar = 50μm. b. Proliferation curve of one normal human mdc preparation (pN1) and one DMD mdc preparation (pD1). The average mean population doubling time for these cells is 33.79±1.24 hrs. c, d, e, f. Representative images showing the expression of NG2 (c, green), CD49b (d, green), CD29 (e, green) and CD44 (f, green) by pN1 human mdcs. Nuclei were counterstained with 10 µg/ml DAPI (blue). Scale bar = 50μm. g. Representative FACS images showing the expression of PDGFRβ, ALP, CD49b, CD29, CD71, CD90, Stro-1, CD56 and CD34 by human mdcs. Isotype control is shown in red in each image, and positive signal is shown in green. Note that most of these markers, apart from PDGFRβ and CD56, were expressed at similar levels among cell preparations
From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011)
Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.
PLoS ONE 6(3): e17454.
Sheep anti Human alkaline phosphatase antibody used for the evaluation of alkaline phosphatase expression in pD2 cells by flowcytometry.
Image caption:
FACS analysis of expression of endothelial markers and double staining of CD56:PE and ALP, CD144, CD34 and CD146 on pD2 cells. a, a': expression of CD146 on pD2 cells. Approximately 76% of cells were CD146+ (a'). Mouse IgG1:FITC was used as isotype control (a) of CD146:FITC antibody. b, b': expression of CD144 on pD2 cells. 0.126% cells were CD144+ (b'). Rabbit IgG:FITC was used as isotype control (b) of CD144:FITC antibody. c, d, e, f: Double staining of pD2 cells with CD56:PE in combination with ALP (c), CD146 (d), CD144 (e) and CD34 (f).
From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011)
Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.
PLoS ONE 6(3): e17454.
Sheep anti Human Alkaline Phosphatase
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- Alkaline Phosphatase
- Region
- (PLAP/BAP)
Antibody Quality and Performance Guarantee
| Sheep anti Human Alkaline Phosphatase antibody recognizes alkaline phosphatase, it has been found to be reactive with both placental and bone alkaline phosphatase. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Purified alkaline phosphatatse from human placenta.
- Approx. Protein Concentrations
- IgG concentration 5.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immuno-electron Microscopy | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
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| List Price | Your Price | ||||||
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| Description | Rabbit anti Sheep IgG (H/L):Biotin | ||||||
References for Alkaline Phosphatase antibody
-
Béaslas, O. et al. (2009) Sensing of dietary lipids by enterocytes: a new role for SR-BI/CLA-1.
PLoS One. 1: e4278 -
Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6: e17454.
- Synonyms
- AP
- RRID
- AB_618548
- UniProt
- P05187
- P05186
- Entrez Gene
- ALPL
- ALPP
- GO Terms
- GO:0005886 plasma membrane
- GO:0004035 alkaline phosphatase activity
- GO:0016021 integral to membrane
- GO:0046872 metal ion binding
- GO:0009986 cell surface
- GO:0031225 anchored to membrane
0300-1909
0512
152717
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