Calretinin antibody

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Sheep anti Guinea Pig Calretinin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1870 C WB 0.1 ml
Sheep anti calretinin antibody recognises calretinin, a ~29 kDa calcium binding protein found mostly in the central nervous system and retina. It is a cytoplasmic protein involved in the regulation of calcium concentration, which is important in the regulation of a large variety of neuronal functions, including neurotransmitter release, ionic channel permeability, enzyme activity, and gene transcription. Mice deficient in calretinin show impairment in motor coordination.

Calretinin is a sensitive and specific marker for mast cells.

Product Details

Target Species
Guinea Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human Expected from Sequence
Mouse Expected from Sequence
Primate Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Calretinin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by Protein A chromatography.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
None present
Immunogen
Purified guinea pig calretinin

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P47728 Related reagents
P22676 Related reagents
Q08331 Related reagents
Entrez Gene
CALB2 Related reagents
Calb2 Related reagents
Calb2 Related reagents
GO Terms
GO:0005509 calcium ion binding
GO:0005921 gap junction
Regulatory
For research purposes only

Applications of Calretinin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/500
Western Blotting 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
AHP1870 detects a band of approximately 29kDa in rat cerebellum.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB