Calretinin antibody
Sheep anti calretinin antibody recognizes calretinin, a ~29 kDa calcium binding protein found mostly in the central nervous system and retina. It is a cytoplasmic protein involved in the regulation of calcium concentration, which is important in the regulation of a large variety of neuronal functions, including neurotransmitter release, ionic channel permeability, enzyme activity, and gene transcription. Mice deficient in calretinin show impairment in motor coordination.
Calretinin is a sensitive and specific marker for mast cells.
Calretinin is a sensitive and specific marker for mast cells.
Product Details
- Target Species
- Guinea Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human Expected from Sequence Mouse Expected from Sequence Primate Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to Calretinin were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG was prepared from whole serum by Protein A chromatography.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Purified guinea pig calretinin
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P47728
- P22676
- Q08331
- Entrez Gene
- CALB2
- Calb2
- Calb2
- GO Terms
- GO:0005509 calcium ion binding
- GO:0005921 gap junction
- Regulatory
- For research purposes only
Applications of Calretinin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/500 | ||
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- AHP1870 detects a band of approximately 29kDa in rat cerebellum.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | C E F WB | 1.5 ml |
|
|
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | F IF | 0.1 mg |
|
|
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | C E P WB | 1 ml |
|
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