IgG antibody

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Donkey anti Goat IgG:HRP (Multi Species Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT1500P C E P WB 0.5 ml
Donkey anti goat IgG antibody recognizes goat immunoglobulin G. This antibody has been absorbed against human, rabbit, rat, horse, guinea pig, hamster, chicken and mouse to minimize naturally occurring cross reactivities.

Product Details

Target Species
Goat
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1.5% Bovine Serum Albumin

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution.

More Information

Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5,000 1/100,000
Immunohistology - Frozen 1/500 1/5,000
Immunohistology - Paraffin 1/500 1/5,000
Western Blotting 1/5,000 1/100,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for IgG antibody

  1. Maas, C. et al. (2010) Activated factor V is a cofactor for the activation of factor XI by thrombin in plasma.
    Proc Natl Acad Sci U S A. 2010 May 18;107(20):9083-7.
  2. Rau, J.C.et al. (2009) Heparin cofactor II in atherosclerotic lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study.
    Exp Mol Pathol. 87: 178-83.
  3. Guitton, C. et al. (2011) Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-κB, and ERK1/2 MAP kinases.
    Am J Physiol Cell Physiol. 300: C833-42.
  4. Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
    Vet Immunol Immunopathol. 159 (1-2): 29-40.
  5. Riesbeck, K. & Forsgren, A. (2016) Interaction of Moraxella catarrhalis with Epithelial Cells, Extracellular Matrix Proteins and the Complement System.
    Patent Application Publication No. 20160318979
  6. Tamarozzi, F. et al. (2014) A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry.
    Immunol Lett. 162 (2 Pt B): 194-8.