IgG antibody
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Donkey anti goat IgG antibody recognizes goat immunoglobulin G. This antibody has been absorbed against human, rabbit, rat, horse, guinea pig, hamster, chicken and mouse to minimize naturally occurring cross reactivities.
Product Details
- Target Species
- Goat
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 1.5% Bovine Serum Albumin
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Shelf Life
- 12 months from date of reconstitution.
More Information
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/5,000 | 1/100,000 | |
| Immunohistology - Frozen | 1/500 | 1/5,000 | |
| Immunohistology - Paraffin | 1/500 | 1/5,000 | |
| Western Blotting | 1/5,000 | 1/100,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| AbGUARD® HRP Stabilizer Plus | BUF052A | 100 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052B | 500 ml | C E P WB | ||
| AbGUARD® HRP Stabilizer Plus | BUF052C | 1000 ml | C E P WB | ||
| TMB Signal+ | BUF054A | 100 ml | E | ||
| TMB Core | BUF056A | 100 ml | E | ||
| TMB Core+ | BUF062A | 100 ml | E |
Product Specific References
References for IgG antibody
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Maas, C. et al. (2010) Activated factor V is a cofactor for the activation of factor XI by thrombin in plasma.
Proc Natl Acad Sci U S A. 2010 May 18;107(20):9083-7. -
Rau, J.C.et al. (2009) Heparin cofactor II in atherosclerotic lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study.
Exp Mol Pathol. 87: 178-83. -
Guitton, C. et al. (2011) Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-κB, and ERK1/2 MAP kinases.
Am J Physiol Cell Physiol. 300: C833-42. -
Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
Vet Immunol Immunopathol. 159 (1-2): 29-40. -
Riesbeck, K. & Forsgren, A. (2016) Interaction of Moraxella catarrhalis with Epithelial Cells, Extracellular Matrix Proteins and the Complement System.
Patent Application Publication No. 20160318979 -
Tamarozzi, F. et al. (2014) A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry.
Immunol Lett. 162 (2 Pt B): 194-8.
