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IgG antibody

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Anti Goat IgG (Multi Species Adsorbed) Antibody thumbnail image 1
Anti Goat IgG (Multi Species Adsorbed) Antibody gallery image 1
Published customer image:
HRP conjugated Donkey anti Goat IgG antibody (OBT1500P) used to detect bound Goat anti Influenza antibody by immunohistochemistry on formalin fixed, paraffin embedded sections following heat mediated antigen retrieval in Tris/EDTA pH9.0.
Image caption:
Effect of latent gammaherpesvirus infection on moderate and severe influenza A infection. Six to eight week old BALB/c mice were infected with 5×104 pfu MHV-68 or mock-infected with BHK cell lysate and 28 days later infected with 5×102 or 5×103 pfu IAV or mock infected with DMEM. (e). Quantified total area2 of lung sections infected with IAV stained with DAB. (f). Representative images of regional IAV DAB staining in lung cross sections. All figures show data from two to three independent experiments, (N=6 or above per group). All data displayed as mean±SEM where */# P<0.05 **/## P<0.01 ***/#### P≤0.001 statistical significance unless otherwise stated.

From:Hardisty G, Nicol MQ, Shaw DJ, Bennet ID, Bryson K, Ligertwood Y, Schwarze J, Beard PM, Hopkins J, Dutia BM.
Latent gammaherpesvirus infection enhances type I IFN response and reduces virus spread in an influenza A virus co-infection model.
J Gen Virol. 2024 Feb;105(2).8 Feb [Epub ahead of print].
doi: 10.1099/jgv.0.001962.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Donkey anti Goat IgG:HRP (Multi Species Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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Product Code Applications Pack Size List Price Your Price Qty
OBT1500P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E P WB 0.5 ml loader
List Price Your Price
loader

Donkey anti goat IgG antibody recognizes goat immunoglobulin G. This antibody has been absorbed against human, rabbit, rat, horse, guinea pig, hamster, chicken and mouse to minimize naturally occurring cross reactivities.

Target Species
Goat
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - lyophilized
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1.5% Bovine Serum Albumin
Approx. Protein Concentrations
Current, batch-specific concentration 0.8 mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5,000 1/100,000
Immunohistology - Frozen 1/500 1/5,000
Immunohistology - Paraffin 1/500 1/5,000
Western Blotting 1/5,000 1/100,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
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TMB Signal+ BUF054A E 100 ml
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TMB Core BUF056A E 100 ml
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References for IgG antibody

  1. Maas, C. et al. (2010) Activated factor V is a cofactor for the activation of factor XI by thrombin in plasma.
    Proc Natl Acad Sci U S A. 2010 May 18;107(20):9083-7.
  2. Rau, J.C.et al. (2009) Heparin cofactor II in atherosclerotic lesions from the Pathobiological Determinants of Atherosclerosis in Youth (PDAY) study.
    Exp Mol Pathol. 87: 178-83.
  3. Guitton, C. et al. (2011) Protective cross talk between activated protein C and TNF signaling in vascular endothelial cells: implication of EPCR, noncanonical NF-κB, and ERK1/2 MAP kinases.
    Am J Physiol Cell Physiol. 300: C833-42.
  4. Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
    Vet Immunol Immunopathol. 159 (1-2): 29-40.
  5. Tamarozzi, F. et al. (2014) A lack of confirmation with alternative assays questions the validity of IL-17A expression in human neutrophils using immunohistochemistry.
    Immunol Lett. 162 (2 Pt B): 194-8.
  6. Nicol, M.Q. et al. (2019) Lack of IFNγ signaling attenuates spread of influenza A virus in vivo and leads to reduced pathogenesis.
    Virology. 526: 155-64.
  7. Hardisty, G. et al. (2024) Latent gammaherpesvirus infection enhances type I IFN response and reduces virus spread in an influenza A virus co-infection model.
    J Gen Virol. 8 Feb [Epub ahead of print].

Immunohistology - Paraffin

RRID
AB_616828

OBT1500P

150354 152654 157477

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