IgG antibody

100% Secure

Donkey anti Goat IgG (H/L):Alk. Phos. (Rat/Mouse Adsorbed)

Donkey anti Goat IgG (H/L):Biotin (Rat/Mouse Adsorbed)

Donkey anti Goat IgG (H/L):FITC (Rat/Mouse Adsorbed)

Donkey anti Goat IgG (H/L):HRP (Rat/Mouse Adsorbed)

Donkey anti Goat IgG (H/L) (Rat/Mouse Adsorbed)

Donkey anti Goat IgG (H/L):RPE (Rat/Mouse Adsorbed)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
642004 C E 1 ml
642008 C E 1 mg
642002 IF 1 mg
642005 C E 1 ml
642001 C E 1 mg
642009 F 0.5 mg
Donkey anti Goat IgG antibody recognizes the heavy and light chains of goat IgG as demonstrated by ELISA. The preparation has minimal cross reaction with rat and mouse serum proteins, however, it may react with the light chains of other goat immunoglobulins.

Product Details

Target Species
Goat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Ig Fraction conjugated to Horseradish Peroxidase (HRP) - liquid
Product Form
Ig Fraction - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Borate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium azide
50% Glycerol
Preservative Stabilisers
0.1% Sodium azide
Preservative Stabilisers
0.1% Sodium azide
Preservative Stabilisers
50% glycerol.
Preservative Stabilisers
None present
Preservative Stabilisers
0.1% Sodium azide
Stabilising agent
Immunogen
Goat IgG.
Approx. Protein Concentrations
0.5 mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
1.0 mg/ml
Approx. Protein Concentrations
0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
-20ºC only (ship +4ºC)
Storage
+4ºC DO NOT FREEZE
This product is photosensitive and should be protected from light.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
Please see label for expiry date.
Shelf Life
12 months from date of despatch.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from the date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
ELISA 1/5,000 1/20,000
Immunohistology - Frozen
Immunofluorescence
ELISA
Immunohistology - Frozen
ELISA
Immunohistology - Frozen
Flow Cytometry 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

Immunofluorescence

Product Specific References

References for IgG antibody

  1. Gerry, A.B. and Leake, D.S. (2014) Effect of low extracellular pH on NF-κB activation in macrophages.
    Atherosclerosis. 233: 537-44.
  2. Armour, K.L.et al. (2014) Clearance of Human IgG1-Sensitised Red Blood Cells In Vivo in Humans Relates to the In Vitro Properties of Antibodies from Alternative Cell Lines.
    PLoS One. 9: e109463.