IgG antibody
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Rabbit F(ab')2 anti goat IgG (H/L) is specific for the heavy and light chains of goat IgG as demonstrated by ELISA. It may also react with the light chains of other goat immunoglobulins.
Product Details
- Target Species
- Goat
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Antiserum Preparation
- Antiserum to Goat IgG (H&L) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were generated by pepsin digestion.
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium azide
50% Glycerol - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- Please see label for expiry date.
- Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2,000 | 1/4,000 | |
| Immunoblotting | |||
| Immunohistology - Frozen | |||
| Western Blotting | |||
| ELISA | 1/200 | 1/400 | |
| Flow Cytometry | Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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