IgG antibody

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Rabbit F(ab')2 anti Goat IgG (H/L):Alk. Phos.

Rabbit F(ab')2 anti Goat IgG (H/L):FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
602004 C E IB WB 1 ml
602002 E F 0.5 mg
Rabbit F(ab')2 anti goat IgG (H/L) is specific for the heavy and light chains of goat IgG as demonstrated by ELISA. It may also react with the light chains of other goat immunoglobulins.

Product Details

Target Species
Goat
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antiserum to Goat IgG (H&L) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were generated by pepsin digestion.
Buffer Solution
TRIS buffered saline, 1mM MgCl2
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium azide
50% Glycerol
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2,000 1/4,000
Immunoblotting
Immunohistology - Frozen
Western Blotting
ELISA 1/200 1/400
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.