IgG antibody
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Rabbit F(ab')2 anti goat IgG (H/L) is specific for the heavy and light chains of goat IgG as demonstrated by ELISA. It may also react with the light chains of other goat immunoglobulins.
Product Details
- Target Species
- Goat
- Product Form
- Purified IgG conjugated to Alkaline Phosphatase - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Antiserum Preparation
- Antiserum to Goat IgG (H&L) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were generated by pepsin digestion.
- Buffer Solution
- TRIS buffered saline, 1mM MgCl2
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium azide
50% Glycerol - Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Guarantee
- Guaranteed until date of expiry. Please see product label.
- Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/2,000 | 1/4,000 | |
| Immunoblotting | |||
| Immunohistology - Frozen | |||
| Western Blotting | |||
| ELISA | 1/200 | 1/400 | |
| Flow Cytometry | Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
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