IgG antibody

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Rabbit F(ab')2 anti Goat IgG (H/L):FITC

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: IgG
Region: (H/L)

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602002 Datasheet Datasheet SDS SDS	E F	0.5 mg		Log in	Get Quote
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Rabbit F(ab')₂ anti goat IgG (H/L) is specific for the heavy and light chains of goat IgG as demonstrated by ELISA. It may also react with the light chains of other goat immunoglobulins.

Target Species: Goat
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation: Antiserum to Goat IgG (H&L) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography. F(ab')2 fragments were generated by pepsin digestion.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: <0.1% Sodium Azide (NaN₃)
Approx. Protein Concentrations: 0.5 mg/ml
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/200	1/400
Flow Cytometry		Neat	1/5

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.