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IgG2 antibody

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Anti Dog IgG2 Antibody thumbnail image 1
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Published customer image:
Horseradish peroxidase conjugated Goat anti Canine IgG2 (AHP948P) antibody used to evaluate the immunoglobulin response to Leishmania infantum promastigotes by sympomatic and asymptomatic dogs by western blotting.
Image caption:
IgG reaction pattern associated with CVL status.
Total Leishmania infantum promastigote cell extracts were separated with 12% SDS-PAGE and electrotransferred to nitrocellulose membranes. The membranes were probed with serum obtained from individual asymptomatic dogs (1:500) or symptomatic dogs (1:1,000) and secondary anti-dog (A) IgG, (B) IgG1 or (C) IgG2 (1:10,000). Representative western blots from each group of dogs are depicted (asymptomatic: n = 5; symptomatic: n = 3). Numbers represent the code numbers of the dogs enrolled in the study. Molecular weight markers are shown on the left-hand side.

From: Agallou M, Athanasiou E, Samiotaki M, Panayotou G, Karagouni E (2016)
Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
PLoS ONE 11(2): e0149894.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Sheep anti Dog IgG2:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG2

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Product Code Applications Pack Size List Price Your Price Qty
AHP948P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E WB 1 mg loader
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Sheep anti Dog IgG2 polyclonal antibody recognizes canine IgG2 by electrophoresis and ELISA. No cross reactivity was detected against other canine immunoglobulin classes, isolated immunoglobulin light chains or no immunoglobulin proteins.

Goat anti Dog IgG2 may cross react with IgG2 from other species.

Target Species
Dog
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to canine IgG2 were raised by repeated immunisations of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.05% ProClin 300
0.2% Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
Immunohistology - Frozen 1/200 1/500
Western Blotting 1/1000 1/10,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG2 antibody

  1. Agallou M et al. (2016) Identification of Immunoreactive Leishmania infantum Protein Antigens to Asymptomatic Dog Sera through Combined Immunoproteomics and Bioinformatics Analysis.
    PLoS One. 11 (2): e0149894.
  2. Martínez Abad, L.P. et al. (2017) Diagnostic accuracy of rKLO8 versus rK26 ELISAs for screening of canine visceral leishmaniasis.
    Acta Trop. 166: 133-8.
  3. Khantavee, N. et al. (2020) Antibody levels to Malassezia pachydermatis. and Staphylococcus pseudintermedius. in atopic dogs and their relationship with lesion scores.
    Vet Dermatol. 31 (2): 111-115.

Further Reading

  1. Bird, R.C. et al. (2011) An autologous dendritic cell canine mammary tumor hybrid-cell fusion vaccine.
    Cancer Immunol Immunother. 60 (1): 87-97.

RRID
AB_567050

AHP948P

149572 154411 158092 158415 167977

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