IgE antibody
Goat anti Dog IgE:FITC
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IgE
Antibody Quality and Performance Guarantee
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| Goat anti Dog IgE antibody recognises canine IgE. The antibody has been shown to react specifically with canine IgE by immunoelectrophoresis and ELISA. Less than 0.01% cross reactivity was detected to canine IgG1, IgG2, IgA and IgM. Goat anti Dog IgE may cross react with IgE from other species. Goat anti Dog IgE antibody has been reported for use in immunohistology of frozen canine sections. |
- Target Species
- Dog
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Antiserum Preparation
- Antisera to canine IgE were raised by repeated immunisations of goats with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin - Immunogen
- Native canine IgE.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/500 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for IgE antibody
-
Krachudel, J. et al. (2013) Luteal insufficiency in bitches as a consequence of an autoimmune response against progesterone?
Theriogenology. 79: 1278-83 -
Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
Vet Immunol Immunopathol. 159: 29-40. -
Elders, R.C. et al. (2014) Recombinant canine IgE Fc and an IgE Fc-TRAIL fusion protein bind to neoplastic canine mast cells.
Vet Immunol Immunopathol. 159 (1-2): 29-40. -
Bizikova, P. et al. (2014) Serum autoantibody profiles of IgA, IgE and IgM in canine pemphigus foliaceus.
Vet Dermatol. 25 (5): 471-e75. -
Couto N et al. (2016) Identification of vaccine candidate antigens of Staphylococcus pseudintermedius by whole proteome characterization and serological proteomic analyses.
J Proteomics. 133: 113-24. -
Moya, R. et al. (2016) Immunoproteomic characterization of a Dermatophagoides farinae extract used in the treatment of canine atopic dermatitis.
Vet Immunol Immunopathol. 180: 1-8. -
Wysmołek, M.E. et al. (2022) Canine antibody response against Dirofilaria repens. in natural occult and microfilaremic infections
Comp Immunol Microbiol Infect Dis. 2 May: 101818 [Epub ahead of print]. -
Karabowicz, J. et al. (2024) Analysis of the role of Dirofilaria repens macrophage migration inhibitory factors in host-parasite interactions.
J Vet Res. 68 (3): 381-8.
View The Latest Product References
-
Sulce, M. et al. (2018) Utility of flow cytometry in canine primary cutaneous and matched nodal mast cell tumor.
Vet J. 242: 15-23.
- RRID
- AB_567016
AHP946F
147877
162388
167967
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