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IgA antibody

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Anti Dog IgA Antibody thumbnail image 1 Anti Dog IgA Antibody thumbnail image 2 Anti Dog IgA Antibody thumbnail image 3 Anti Dog IgA Antibody thumbnail image 4
Anti Dog IgA Antibody gallery image 1
Published customer image:
Goat anti Dog IgA antibody used for the measurement of serum IgA levels in dogs with atopic dermatitis by ELISA.
Image caption:
Correlation between the phenotypes and obvious population structure was detected in the GSD population. The difference in IgA levels (pdiff = 1.1×10−5, based on Welsh two sample t-test) in CAD cases and CAD controls (Ntotal = 184, before removing CAD controls with low IgA levels) is presented with boxplots (A). The GSD population is visualized with an MDS-plot displaying the formation of two subpopulations (B) with the uneven distribution of CAD cases and CAD controls (C). The distribution of IgA levels across the subpopulations is visualized with violin plots (D). Panels B-D include all dogs after QC (n = 203) and the dotted lines on the violin plots (A and D) correspond to the respective median values.

From: Tengvall K, Kierczak M, Bergvall K, Olsson M, Frankowiack M, et al. (2013)
Genome-Wide Analysis in German Shepherd Dogs Reveals Association of a Locus on CFA 27 with Atopic Dermatitis.
PLoS Genet 9(5): e1003475.
Anti Dog IgA Antibody gallery image 2
Published customer image:
Sheep anti Dog IgA polyclonal antibody (AAI31F) used to stain bacteria carryiing canine IgA by flow cytometry.
Image caption:
Representative staining of CE during active disease and remission.
For microbial fraction identification the trigger was set up based on side scatter properties. Then, singlets were selected based on SSC-width vs SCC-height. FITC-negative window population was set up in samples containing only SYTO 17 (A). These windows were applied to samples stained with FITC-IgA (B) or FITC-IgG (C) to distinguish the negative population from the positive one.

From: Martínez-López LM, Perez-Gonzalez A, Washington EA, Woodward AP, Roth-Schulze AJ, Dandrieux JRS, et al. (2021)
Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
PLoS ONE 16(8): e0255012.
doi:10.1371/journal.pone.0255012
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Dog IgA Antibody gallery image 3
Published customer image:
Mouse anti Dog IgA antibody, clone K117 1A8 (MCA631GA) used as a capture reagent in a sandwich ELISA with HRP conjugated anti canine IgA (AAI31P) as a detection reagent.
Image caption:
Immunoglobulin IgA titers in fecal contents collected at the beginning of the study (T0), after 2 weeks (T2), 4 weeks (T4), 6 weeks (T6), and 8 weeks (T8) in dogs fed the same diet with (Group A) or without Slab51® probiotic supplement (Group B). Black hashtags indicate statistical significance between groups. Red asterisks indicate statistical significance by study times within group A.

From: Rossi G, Pengo G, Galosi L, Berardi S, Tambella AM, Attili AR, Gavazza A, Cerquetella M, Jergens AE, Guard BC, Lidbury JA, Stainer JM, Crovace AM and Suchodolski JS (2020)
Effects of the Probiotic Mixture Slab51® (SivoMixx®) as Food Supplement in Healthy Dogs: Evaluation of Fecal Microbiota, Clinical Parameters and Immune Function.
Front. Vet. Sci. 7:613.
doi: 10.3389/fvets.2020.00613
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Dog IgA Antibody gallery image 4
Published customer image:
HRP conjugated Goat anti canine IgA antibody (AAI31P) used as a detection reagent in a sandwich ELISA with Mouse anti Canine IgA antibody, clone K117 1A8 ( MCA631GA) as a capture reagent.
Image caption:
GALT response: Changes in fecal IgA in each group during the trial are depicted as mean fecal IgA values ± SEM. X-axis depicts time in weeks and Y-axis depicts mean fecal IgA values in μg/ml. #p <0.05.

From: Satyaraj E, Reynolds A, Engler R, Labuda J, Sun P.
Supplementation of Diets With Spirulina Influences Immune and Gut Function in Dogs.
Front Nutr. 2021 May 28;8:667072.
doi: 10.3389/fnut.2021.667072.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Dog IgA:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgA

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AAI31F
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Goat anti Dog IgA polyclonal antibody recognizes canine IgA and shows no cross reactivity with other canine immunoglobulin classes in immunoelectrophoresis or ELISA.

Goat anti Dog IgA may cross react with IgA from other species. Goat anti Dog IgA polyclonal antibody has been shown to cross react with IgA from other canids (Frankowiack et al. 2013) and with mustelids (Martel et al. 2009).

Target Species
Dog
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mustelid Expected from Sequence
Ferret
Wolf
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to canine IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Purified canine IgA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/50 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

References for IgA antibody

  1. Benyacoub, J. et al. (2003) Supplementation of food with Enterococcus faecium (SF68) stimulates immune functions in young dogs.
    J Nutr. 133 (4): 1158-62.
  2. Peters, I.R. et al. (2004) Measurement of immunoglobulin concentrations in the feces of healthy dogs.
    Clin Diagn Lab Immunol. 11 (5): 841-8.
  3. Simpson, K.W. et al. (2009) Influence of Enterococcus faecium SF68 probiotic on giardiasis in dogs.
    J Vet Intern Med. 23: 476-81.
  4. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  5. Huang, S.S. et al. (2012) Differential Pathological and Immune Responses in Newly Weaned Ferrets Are Associated with a Mild Clinical Outcome of Pandemic 2009 H1N1 Infection.
    J Virol. 86: 13187-201.
  6. Frankowiack, M. et al. (2013) IgA deficiency in wolves.
    Dev Comp Immunol. 40 (2): 180-4.
  7. Olsson M et al. (2014) The dog as a genetic model for immunoglobulin A (IgA) deficiency: identification of several breeds with low serum IgA concentrations.
    Vet Immunol Immunopathol. 160 (3-4): 255-9.
  8. Frankowiack, M. et al. (2015) IgA deficiency in wolves from Canada and Scandinavia.
    Dev Comp Immunol. 50 (1): 26-8.
    9. View The Latest Product References
  9. Lee, A. et al. (2015) Lack of correlation between mucosal immunoglobulin A-positive plasma cell numbers and TLR5 genotypes in German shepherd dogs with idiopathic chronic enteropathy.
    J Comp Pathol. 152 (2-3): 201-5.
  10. Tengvall, K. et al. (2013) Genome-wide analysis in German shepherd dogs reveals association of a locus on CFA 27 with atopic dermatitis.
    PLoS Genet. 9 (5): e1003475.
  11. Martínez-López, L.M. et al. (2021) Hierarchical modelling of immunoglobulin coated bacteria in dogs with chronic enteropathy shows reduction in coating with disease remission but marked inter-individual and treatment-response variability.
    PLoS One. 16 (8): e0255012.
  12. Sfacteria, A. et al. (2021) Immune Cells and Immunoglobulin Expression in the Mammary Gland Tumors of Dog.
    Animals (Basel). 11(5):1189.
  13. Rossi, G. et al. (2020) Effects of the Probiotic Mixture Slab51® (SivoMixx®) as Food Supplement in Healthy Dogs: Evaluation of Fecal Microbiota, Clinical Parameters and Immune Function.
    Front Vet Sci. 7: 613.
  14. Satyaraj, E. et al. (2021) Supplementation of Diets With Spirulina Influences Immune and Gut Function in Dogs.
    Front Nutr. 8: 667072.

ELISA

Immunohistology - Paraffin

Western Blotting

RRID
AB_323058

AAI31F

153792 162602 170522

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