IL-1 Beta antibody
Biotinylated Rabbit anti Bovine interleukin 1β antibody (AHP851B) used for the detection of IL-1β using a sandwich ELISA with cross-reactive Mouse anti Ovine IL-1&beta antibody (MCA1658) as a capture reagent
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in bovine experimental S. epidermidis (-○-) and S. simulans (-●-) intramammary infection during the two-week study period (mean ± SEM, n = 8 + 8; TNF-α n = 8 + 7). Milk IL-8 (p = 0.04) and IL-1β (p = 0.03) concentrations were higher in response to infection with S. simulans than with S. epidermidis. The SCC is expressed as a natural logarithm of cells/mL.
From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S.
Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.
Biotinylated Rabbit anti Bovine interleukin 1β antibody (AHP851B) used for the detection of IL-1β using a sandwich ELISA with cross-reactive Mouse anti Ovine IL-1&beta antibody (MCA1658) as a capture reagent
Image caption:
Somatic cell count (a) and concentrations of IL-1β (b), TNF-α (c) and IL-8 (d) in the milk in persistent (-□-) and spontaneously eliminated (-■-) S. epidermidis and S. simulans intramammary infections during the two-week study period (mean ± SEM, n = 11 persisted infections and 5 spontaneously eliminated infections; TNF-α n = 10 + 5). The SCC is expressed as a natural logarithm (ln) of cells/mL.
From: Simojoki H, Salomäki T, Taponen S, Iivanainen A, Pyörälä S.
Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 2011 Mar 17;42:49.
Rabbit anti Bovine Interleukin-1 beta antibody recognizes bovine interleukin-1 beta (IL-1 beta).
IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the bovine immune response.
Rabbit anti Bovine Interleukin-1 beta antibody recognizes both native and recombinant bovine IL-1 beta. No detectable cross-reactivity was observed with human, mouse or rat IL-1 beta.
IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the bovine immune response.
Rabbit anti Bovine Interleukin-1 beta antibody recognizes both native and recombinant bovine IL-1 beta. No detectable cross-reactivity was observed with human, mouse or rat IL-1 beta.
Product Details
- Target Species
- Bovine
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to Biotin - lyophilised
- Reconstitution
- Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the antibody may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant bovine IL-1 beta.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.25mg/ml after reconstitution
Storage Information
- Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P09428 Related reagents
- Entrez Gene
- IL1B Related reagents
- GO Terms
- GO:0001660 fever generation
- GO:0005125 cytokine activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
- GO:0051781 positive regulation of cell division
- Regulatory
- For research purposes only
Applications of IL-1 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1ug/ml | 10ug/ml | |
| ELISA | 1ug/ml | 10ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This antibody may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA system, in combination with biotinylated Rabbit anti Bovine interleukin-21 antibody (AHP851B) as the detection antibody. Recombinant bovine interleukin-21 (PBP008) may be used as a standard.
- ELISA
- This anti interleukin-1β:biotin conjugate may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with recombinant IL-1β (PBP008) as the standard and either Rabbit anti Bovine IL-1β (AHP851Z) or purified Mouse anti Ovine IL-1β (MCA1658) as the capture reagents.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Sheep anti Rabbit IgG:Biotin | 2AB02B | 1 ml | C E P WB | ||
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | 1 mg | E WB | ||
| Goat anti Rabbit IgG (Fc):FITC | STAR121F | 1 mg | F | ||
| Goat anti Rabbit IgG (Fc):HRP | STAR121P | 1 mg | E WB | ||
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | 1 mg | C E WB | ||
| Sheep anti Rabbit IgG:FITC | STAR34B | 1 mg | C F | ||
| Sheep anti Rabbit IgG:RPE | STAR35A | 1 ml | F | ||
| Sheep anti Rabbit IgG:DyLight®488 | STAR36D488GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:DyLight®549 | STAR36D549GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®649 | STAR36D649GA | 0.1 mg | F IF | ||
| Sheep anti Rabbit IgG:DyLight®680 | STAR36D680GA | 0.1 mg | F IF WB | ||
| Sheep anti Rabbit IgG:Dylight®800 | STAR36D800GA | 0.1 mg | F IF WB |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Bovine Interleukin-1 beta:Biotin | AHP851B | 0.25 mg | E | ||
| Rabbit anti Bovine Interleukin-1 beta:Preservative Free | AHP851Z | 0.5 mg | E |
Product Specific References
References for IL-1 Beta antibody
-
Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
J Dairy Sci. 93: 2458-70. -
Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
Funct Integr Genomics. 10: 21-38. -
Bougarn, S. et al. (2010) Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.
Clin Vaccine Immunol. 17 (11): 1797-809. -
Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
Vet Res. 42: 49. -
Harte,C. et al. (2017) Alum Activates the Bovine NLRP3 Inflammasome
Frontiers in Immunology. 8 [Epub ahead of print].
