IL-1 Beta antibody

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Rabbit anti Bovine Interleukin-1 beta:Preservative Free

Rabbit anti Bovine Interleukin-1 beta:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP851Z E 0.5 mg
AHP851B E 0.25 mg
Rabbit anti Bovine Interleukin-1 beta antibody recognizes bovine interleukin-1 beta (IL-1 beta).

IL-1 beta is principally expressed by macrophages, and plays an important immunomodulatory role in the bovine immune response.

Rabbit anti Bovine Interleukin-1 beta antibody recognizes both native and recombinant bovine IL-1 beta. No detectable cross-reactivity was observed with human, mouse or rat IL-1 beta.

Product Details

Target Species
Bovine
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to Biotin - lyophilised
Reconstitution
Reconstitute with 1.0ml distilled water
Care should be taken during reconstitution as the antibody may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Preservative Stabilisers
None present
Immunogen
Recombinant bovine IL-1 beta.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.25mg/ml after reconstitution

Storage Information

Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P09428 Related reagents
Entrez Gene
IL1B Related reagents
GO Terms
GO:0001660 fever generation
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
GO:0051781 positive regulation of cell division
Regulatory
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1ug/ml 10ug/ml
ELISA 1ug/ml 10ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

ELISA
This antibody may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA system, in combination with biotinylated Rabbit anti Bovine interleukin-21 antibody (AHP851B) as the detection antibody. Recombinant bovine interleukin-21 (PBP008) may be used as a standard.
ELISA
This anti interleukin-1β:biotin conjugate may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with recombinant IL-1β (PBP008) as the standard and either Rabbit anti Bovine IL-1β (AHP851Z) or purified Mouse anti Ovine IL-1β (MCA1658) as the capture reagents.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Sheep anti Rabbit IgG:Biotin 2AB02B 1 ml C E P WB
Goat anti Rabbit IgG (Fc):Biotin STAR121B 1 mg E WB
Goat anti Rabbit IgG (Fc):FITC STAR121F 1 mg F
Goat anti Rabbit IgG (Fc):HRP STAR121P 1 mg E WB
Goat anti Rabbit IgG (H/L):HRP STAR124P 1 mg C E WB
Sheep anti Rabbit IgG:FITC STAR34B 1 mg C F
Sheep anti Rabbit IgG:RPE STAR35A 1 ml F
Sheep anti Rabbit IgG:DyLight®488 STAR36D488GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:DyLight®549 STAR36D549GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®649 STAR36D649GA 0.1 mg F IF
Sheep anti Rabbit IgG:DyLight®680 STAR36D680GA 0.1 mg F IF WB
Sheep anti Rabbit IgG:Dylight®800 STAR36D800GA 0.1 mg F IF WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Bovine Interleukin-1 beta:Biotin AHP851B 0.25 mg E
Rabbit anti Bovine Interleukin-1 beta:Preservative Free AHP851Z 0.5 mg E

Product Specific References

References for IL-1 Beta antibody

  1. Wenz, J.R. et al. (2010) Factors associated with concentrations of select cytokine and acute phase proteins in dairy cows with naturally occurring clinical mastitis.
    J Dairy Sci. 93: 2458-70.
  2. Rinaldi, M. et al (2010) A sentinel function for teat tissues in dairy cows: dominant innate immune response elements define early response to E. coli mastitis.
    Funct Integr Genomics. 10: 21-38.
  3. Bougarn, S. et al. (2010) Muramyl dipeptide synergizes with Staphylococcus aureus lipoteichoic acid to recruit neutrophils in the mammary gland and to stimulate mammary epithelial cells.
    Clin Vaccine Immunol. 17 (11): 1797-809.
  4. Simojoki, H. et al. (2011) Innate immune response in experimentally induced bovine intramammary infection with Staphylococcus simulans and S. epidermidis.
    Vet Res. 42: 49.
  5. Harte,C. et al. (2017) Alum Activates the Bovine NLRP3 Inflammasome
    Frontiers in Immunology. 8 [Epub ahead of print].