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IgG antibody

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Anti Chicken IgG (Fc) Antibody thumbnail image 1 Anti Chicken IgG (Fc) Antibody thumbnail image 2 Anti Chicken IgG (Fc) Antibody thumbnail image 3
Anti Chicken IgG (Fc) Antibody gallery image 1
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgG (IgY) antibody (AAI29P) used for the evaluation of flagellin specific IgG (IgY) response in exposed mice by ELISA.
Image caption:
Antigen-specific serum antibody responses.
NHC flagellin-specific antibody levels in the groups immunized with (A) 40 μg of NHC protein and (B) 20 μg of NHC protein, both compared to levels in the solvent injected control group. Antigen-specific IgY, IgM or IgA antibody levels were determined in 20-fold diluted sera collected from 12 day-old individual animals. Results are displayed as a box-and-whisker plot (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between compared groups.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Chicken IgG (Fc) Antibody gallery image 2
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgG (IgY) antibody (AAI29P) used for the evaluation of flagellin specific IgG (IgY) response in exposed mice by ELISA.
Image caption:
Kinetics of NHC-specific antibody levels following in ovo vaccination.
ELISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between groups.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Chicken IgG (Fc) Antibody gallery image 3
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgG (IgY) antibody used for the evaluation of flagellin specific IgG (IgY) response in exposed mice by western blotting.
Image caption:
Western blot analysis of chicken sera.
Purified flagellins (FlaA, NHC) and whole cell lysates of
C. jejuni
81116 (wild type and ΔflaAB) were analyzed by Western blotting. Pooled sera collected from 18 day-old chickens injected with (A) NHC flagellin (40 μg) or (B) solvent were used as probes and detected with anti-chicken IgY antibody. Molecular mass is indicated in kilodaltons (kDa). The arrowhead indicates the reactivity of the sera with the native C. jejuni FlaA flagellin that is absent in the ΔflaAB mutant strain.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Goat anti Chicken IgG (Fc):HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(Fc)

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Product Code Applications Pack Size List Price Your Price Qty
AAI29P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 1 mg loader
List Price Your Price
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Goat anti Chicken IgG (Fc) antibody recognizes the heavy chains of chicken IgG, specifically, epitopes within the Fc region and shows no cross-reactivity with other chicken immunoglobulin classes as assessed by immunoelectrophoresis. This Goat anti Chicken IgG polyclonal antibody does not react with the light chains of chicken IgG.

Goat anti chicken IgG (Fc) has been used successfully for the evaluation of circulating levels of IgG in chickens using ELISA in a number of experimental and field situations.

Target Species
Chicken
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to chicken IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.05% ProClin 300
0.2% Bovine Serum Albumin
Immunogen
Purified chicken IgG (Fc) fragment.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
ProClin is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/10,000 1/100,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
List Price Your Price
Description TMB Signal+
TMB Core BUF056A E 100 ml
List Price Your Price
Description TMB Core
TMB Core+ BUF062A E 100 ml
List Price Your Price
Description TMB Core+

References for IgG antibody

  1. Duckworth, J.A. et al. (2008) Development of a contraceptive vaccine for the marsupial brushtail possum (Trichosurus vulpecula): lack of effects in mice and chickens immunised with recombinant possum ZP3 protein and a possum ZP3 antifertility epitope
    Wildlife Research 35, 563–72.
  2. Peralta, B. et al. (2009) Evidence of widespread infection of avian hepatitis E virus (avian HEV) in chickens from Spain.
    Vet Microbiol. 137: 31-6
  3. Norup, L.R. et al. (2009) Influence of chicken serum mannose-binding lectin levels on the immune response towards Escherichia coli.
    Poult Sci. 88:543-53.
  4. Ferdushy, T. et al. (2014) Acquisition of resistance after continuous infection with Ascaridia galli. in chickens.
    Parasitology. : 1-8.
  5. Pleidrup, J. et al. (2014) Ascaridia galli infection influences the development of both humoral and cell-mediated immunity after Newcastle Disease vaccination in chickens.
    Vaccine. 32 (3): 383-92.
  6. Cho Y et al. (2015) Proteomic analysis of outer membrane proteins in Salmonella enterica Enteritidis.
    J Microbiol Biotechnol. 25 (2): 288-95.
  7. Radomska KA et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
    PLoS One. 11 (10): e0164837.
  8. Ranchod, H. et al. (2018) The antigenicity and cholesteroid nature of mycolic acids determined by recombinant chicken antibodies.
    PLoS One. 13 (8): e0200298.
    9. View The Latest Product References
  9. Vaezirad, M.M. et al. (2018) Chicken immune response following in ovo delivery of bacterial flagellin.
    Vaccine. 36 (16): 2139-46.
  10. Naghizadeh, M. et al. (2019) Rapid whole blood assay using flow cytometry for measuring phagocytic activity of chicken leukocytes.
    Vet Immunol Immunopathol. 207: 53-61.
  11. Al-karagoly, H. et al. (2019) Turkey humoral and cell-mediated immune responses to a Newcastle viscerotropic vaccine and its association with major histocompatibility complex.
    Bulg J Vet Med. 22 (1): 26-40.
  12. Tang, B. et al. (2020) GtxA is a virulence factor that promotes a Th2-like response during Gallibacterium anatis. infection in laying hens.
    Vet Res. 51 (1): 40.

Synonyms
IgY
RRID
AB_323045

AAI29P

151818 160151 161069

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