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IgA antibody

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Anti Chicken IgA Antibody thumbnail image 1 Anti Chicken IgA Antibody thumbnail image 2 Anti Chicken IgA Antibody thumbnail image 3 Anti Chicken IgA Antibody thumbnail image 4 Anti Chicken IgA Antibody thumbnail image 5
Anti Chicken IgA Antibody gallery image 1
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgA antibody (AAI28P) used for the measurement of IgA levels by ELISA.
Image caption:
Antibody levels in APEC- and mock-infected birds following primary infection and after homologous re-challenge. The levels were measured by ELISA using different antigens; IgY levels against soluble χ7122nalR antigens (A), IgY levels against O78 LPS purified from χ7122nalR (B) and IgA levels measured against soluble χ7122nalR antigens (C). At 0, 7, 14, 21, and 28 days post-primary infection, five birds per group were sampled and ten birds per group were sampled after re-challenge (day 42). The data in panels B and C reflect levels at post-mortem examination on day 42, one day after re-challenge. * = P ≤ 0.05; ** = P ≤ 0.01.

From: Sadeyen JR, Kaiser P, Stevens MP, Dziva F.
Analysis of immune responses induced by avian pathogenic Escherichia coli infection in turkeys and their association with resistance to homologous re-challenge.
Vet Res. 2014 45:19.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Chicken IgA Antibody gallery image 2
Published customer image:
Alkaline phosphatase conjugated Goat anti Chicken IgA antibody used for the evaluation of IgA levels in chickens by ELISA
Image caption:
Antigen-specific serum IgM (A), IgA (B), and IgG (C) against S. Virchow and S. Typhimurium at 5, 11, and 26 DPI, following infection of 7-day old chickens. Data shown are based on five birds per group at each time point. Error bars represent the standard error of the mean. An asterisk indicates that there was a significant difference between the S. Virchow infected group and uninfected group (*) or the S. Typhimurium infected group and the uninfected group (**) (P < 0.05).

From: Salisbury A-M, Leeming G, Nikolaou G, Kipar A and Wigley P (2014)
Salmonella Virchow infection of the chicken elicits cellular and humoral systemic and mucosal responses, but limited protection to homologous or heterologous re-challenge.
Front. Vet. Sci. 1:6.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Chicken IgA Antibody gallery image 3
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgA antibody used for the evaluation of flagellin specific IgA response in exposed chickens by ELISA.
Image caption:
Antigen-specific serum antibody responses.
NHC flagellin-specific antibody levels in the groups immunized with (A) 40 μg of NHC protein and (B) 20 μg of NHC protein, both compared to levels in the solvent injected control group. Antigen-specific IgY, IgM or IgA antibody levels were determined in 20-fold diluted sera collected from 12 day-old individual animals. Results are displayed as a box-and-whisker plot (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between compared groups.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Chicken IgA Antibody gallery image 4
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgM antibody used for the evaluation of flagellin specific IgM response in exposed chickens by ELISA.
Image caption:
Kinetics of NHC-specific antibody levels following in ovo vaccination.
ELISA results demonstrating the NHC flagellin-specific reactivity of serially diluted sera collected from (A, C, E) 12 day-old and (B, D, F) 18 day-old chickens injected with 40 μg of NHC flagellin or solvent (control). Serum IgY (A, B), IgM (C, D) and IgA (E, F) antibody responses are indicated. Graphs represent the analysis of individual animals, displayed as a box-and-whisker plot. The amount of antigen-specific antibodies was expressed in a relation to the reference sera (S/R, sample to reference ratio). An asterisk indicates statistically significant differences (α<0.05) between groups.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Chicken IgA Antibody gallery image 5
Published customer image:
Horseradish peroxidase conjugated Goat anti Chicken IgM antibody used for the evaluation of flagellin specific IgM response in exposed chickens by ELISA.
Image caption:
Cecal colonization of C. jejuni and sIgA responses in vaccinated and control chickens.(A) Chickens were challenged with C. jejuni at 18 day of age. One week post-inoculation (25 days of age) the number C. jejuni in the ceca was determined by plate counting (CFU/ g). Each symbol represents one chicken of the indicated groups, bars represent the mean number of log10 CFU/ g per group. (B) Antigen-specific sIgA in 20-times diluted cecal content from 25 day-old chickens (one week after C. jejuni challenge) as determined by ELISA. Graphs represent the analysis of individual animals of each of the indicated groups, displayed as a box-and-whisker plot.

From: Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM (2016)
Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
PLoS ONE 11(10): e0164837.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Goat anti Chicken IgA:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgA

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Goat anti Chicken IgA antibody recognizes chicken immunoglobulin A and shows no cross-reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.

Goat anti Chicken IgA antibody may react with IgA from other species.

Target Species
Chicken
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to chicken IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Purified chicken IgA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/200 1/2,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

References for IgA antibody

  1. Withanage, G.S. et al. (2005) Cytokine and chemokine responses associated with clearance of a primary Salmonella enterica serovar Typhimurium infection in the chicken and in protective immunity to rechallenge.
    Infect Immun. 73 (8): 5173-82.
  2. Singh, R. (2010) Immunogenicity and protective efficacy of virosome based vaccines against Newcastle disease.
    Trop Anim Health Prod. 42: 465-71
  3. Wyszyśska A et al. (2004) Oral immunization of chickens with avirulent Salmonella vaccine strain carrying C. jejuni 72Dz/92 cjaA gene elicits specific humoral immune response associated with protection against challenge with wild-type Campylobacter.
    Vaccine. 22 (11-12): 1379-89.
  4. Beal, R.K. et al. (2005) A strong antigen-specific T-cell response is associated with age and genetically dependent resistance to avian enteric salmonellosis.
    Infect Immun. 73: 7509-16.
  5. Buckley, A.M. et al. (2010) Evaluation of live-attenuated Salmonella vaccines expressing Campylobacter antigens for control of C. jejuni in poultry.
    Vaccine. 28: 1094-105.
  6. Bérto Letícia Dal et al. (2015) Live and Inactivated Salmonella enteritidis Vaccines: Immune Mechanisms in Broiler Breeders
    World Journal of Vaccines. 05 (04): 155-164.
  7. Beal, R.K. et al. (2004) Age at primary infection with Salmonella enterica serovar Typhimurium in the chicken influences persistence of infection and subsequent immunity to re-challenge.
    Vet Immunol Immunopathol. 100 (3-4): 151-64.
  8. Park, S.I. et al. (2010) Immune response induced by ppGpp-defective Salmonella enterica serovar Gallinarum in chickens.
    J Microbiol. 48 (5): 674-81.
    9. View The Latest Product References
  9. Beal RK et al. (2004) Temporal dynamics of the cellular, humoral and cytokine responses in chickens during primary and secondary infection with Salmonella enterica serovar Typhimurium.
    Avian Pathol. 33 (1): 25-33.
  10. Zhang L et al. (2008) Enhancement of mucosal immune responses by intranasal co-delivery of Newcastle disease vaccine plus CpG oligonucleotide in SPF chickens in vivo.
    Res Vet Sci. 85 (3): 495-502.
  11. Park, E.H. et al. (2014) Protective efficacy of a single dose of baculovirus hemagglutinin-based vaccine in chickens and ducks against homologous and heterologous H5N1 virus infections.
    Viral Immunol. 27 (9): 449-62.
  12. Barrow, P.A. et al. (2004) Faecal shedding and intestinal colonization of Salmonella enterica in in-bred chickens: the effect of host-genetic background.
    Epidemiol Infect. 132 (1): 117-26.
  13. Andersen, J.P. et al. (2013) No protection in chickens immunized by the oral or intra-muscular immunization route with Ascaridia galli soluble antigen.
    Avian Pathol. 42 (3): 276-82.
  14. Koppad, S. et al. (2011) Calcium phosphate coupled Newcastle disease vaccine elicits humoral and cell mediated immune responses in chickens.
    Res Vet Sci. 91 (3): 384-90.
  15. Rezar, V. et al. (2007) Dose-dependent effects of T-2 toxin on performance, lipid peroxidation, and genotoxicity in broiler chickens.
    Poult Sci. 86 (6): 1155-60.
  16. Sadeyen JR et al. (2014) Analysis of immune responses induced by avian pathogenic Escherichia coli infection in turkeys and their association with resistance to homologous re-challenge.
    Vet Res. 45: 19.
  17. Barman, N. N. et al. (2014) Reflection of serum immunoglobulin isotypes in the egg yolk of laying hens immunized with enterotoxigenic Escherichia coli
    Veterinary World. 7 (9): 749-53.
  18. Salisbury Anne-Marie et al. (2014) Salmonella Virchow Infection of the Chicken Elicits Cellular and Humoral Systemic and Mucosal Responses, but Limited Protection to Homologous or Heterologous Re-Challenge
    Frontiers in Veterinary Science. 1: 6.
  19. Radomska, K.A. et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
    PLoS One. 11 (10): e0164837.
  20. Beir˜o, B.C.B. et al. (2018) Effect of an Enterococcus faecium. probiotic on specific IgA following live Salmonella enteritidis. vaccination of layer chickens.
    Avian Pathol. 47 (3): 325-33.
  21. Al-Karagoly, H. et al. (2019) Turkey humoral and cell-mediated immune responses to a Newcastle viscerotropic vaccine and its association with major histocompatibility complex.
    Bulg J Vet Med. 22 (1): 26-40.

ELISA

RRID
AB_323050

AAI28F

147085 158084 162156

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