IgA antibody

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Goat anti Chicken IgA:Alk. Phos.

Goat anti Chicken IgA:FITC

Goat anti Chicken IgA:HRP

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AAI28AB E 0.5 mg
AAI28F C F 1 mg
AAI28P E 1 mg
Goat anti Chicken IgA antibody recognizes chicken immunoglobulin A and shows no cross-reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.

Goat anti Chicken IgA antibody may react with IgA from other species.

Product Details

Target Species
Chicken
Product Form
Purified IgG conjugated to Alkaline Phosphatase - liquid
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Antiserum Preparation
Antisera to chicken IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
0.2% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Preservative Stabilisers
0.1% Proclin™ 300
0.2% Bovine Serum Albumin
Immunogen
Purified chicken IgA.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC.
DO NOT FREEZE.

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.
Shelf Life
12 months from date of despatch.

More Information

Acknowledgements
Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Flow Cytometry
Immunohistology - Frozen 1/200 1/2,000
ELISA 1/10,000 1/100,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Application Based External Images

ELISA

Product Specific References

References for IgA antibody

  1. Withanage, G.S. et al. (2005) Cytokine and chemokine responses associated with clearance of a primary Salmonella enterica serovar Typhimurium infection in the chicken and in protective immunity to rechallenge.
    Infect Immun. 73 (8): 5173-82.
  2. Singh, R. (2010) Immunogenicity and protective efficacy of virosome based vaccines against Newcastle disease.
    Trop Anim Health Prod. 42: 465-71
  3. Wyszyśska A et al. (2004) Oral immunization of chickens with avirulent Salmonella vaccine strain carrying C. jejuni 72Dz/92 cjaA gene elicits specific humoral immune response associated with protection against challenge with wild-type Campylobacter.
    Vaccine. 22 (11-12): 1379-89.
  4. Beal, R.K. et al. (2005) A strong antigen-specific T-cell response is associated with age and genetically dependent resistance to avian enteric salmonellosis.
    Infect Immun. 73: 7509-16.
  5. Buckley, A.M. et al. (2010) Evaluation of live-attenuated Salmonella vaccines expressing Campylobacter antigens for control of C. jejuni in poultry.
    Vaccine. 28: 1094-105.
  6. Bérto Letícia Dal et al. (2015) Live and Inactivated Salmonella enteritidis Vaccines: Immune Mechanisms in Broiler Breeders
    World Journal of Vaccines. 05 (04): 155-164.
  7. Beal, R.K. et al. (2004) Age at primary infection with Salmonella enterica serovar Typhimurium in the chicken influences persistence of infection and subsequent immunity to re-challenge.
    Vet Immunol Immunopathol. 100 (3-4): 151-64.
  8. Park, S.I. et al. (2010) Immune response induced by ppGpp-defective Salmonella enterica serovar Gallinarum in chickens.
    J Microbiol. 48 (5): 674-81.
  9. Beal RK et al. (2004) Temporal dynamics of the cellular, humoral and cytokine responses in chickens during primary and secondary infection with Salmonella enterica serovar Typhimurium.
    Avian Pathol. 33 (1): 25-33.
  10. Zhang L et al. (2008) Enhancement of mucosal immune responses by intranasal co-delivery of Newcastle disease vaccine plus CpG oligonucleotide in SPF chickens in vivo.
    Res Vet Sci. 85 (3): 495-502.
  11. Park, E.H. et al. (2014) Protective efficacy of a single dose of baculovirus hemagglutinin-based vaccine in chickens and ducks against homologous and heterologous H5N1 virus infections.
    Viral Immunol. 27 (9): 449-62.
  12. Barrow, P.A. et al. (2004) Faecal shedding and intestinal colonization of Salmonella enterica in in-bred chickens: the effect of host-genetic background.
    Epidemiol Infect. 132 (1): 117-26.
  13. Andersen, J.P. et al. (2013) No protection in chickens immunized by the oral or intra-muscular immunization route with Ascaridia galli soluble antigen.
    Avian Pathol. 42 (3): 276-82.
  14. Koppad, S. et al. (2011) Calcium phosphate coupled Newcastle disease vaccine elicits humoral and cell mediated immune responses in chickens.
    Res Vet Sci. 91 (3): 384-90.
  15. Rezar, V. et al. (2007) Dose-dependent effects of T-2 toxin on performance, lipid peroxidation, and genotoxicity in broiler chickens.
    Poult Sci. 86 (6): 1155-60.
  16. Sadeyen JR et al. (2014) Analysis of immune responses induced by avian pathogenic Escherichia coli infection in turkeys and their association with resistance to homologous re-challenge.
    Vet Res. 45: 19.
  17. Barman, N. N. et al. (2014) Reflection of serum immunoglobulin isotypes in the egg yolk of laying hens immunized with enterotoxigenic Escherichia coli
    Veterinary World. 7 (9): 749-753.
  18. Salisbury Anne-Marie et al. (2014) Salmonella Virchow Infection of the Chicken Elicits Cellular and Humoral Systemic and Mucosal Responses, but Limited Protection to Homologous or Heterologous Re-Challenge
    Frontiers in Veterinary Science. 1: 6.
  19. Radomska, K.A. et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
    PLoS One. 11 (10): e0164837.