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IgA antibody

Goat anti Chicken IgA:FITC

Product Type
Polyclonal Antibody
Polyclonal IgG

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Goat anti Chicken IgA antibody recognizes chicken immunoglobulin A and shows no cross-reactivity with other chicken immunoglobulin classes in immunoelectrophoresis.

Goat anti Chicken IgA antibody may react with IgA from other species.

Target Species
Product Form
Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to chicken IgA were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Purified chicken IgA.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/200 1/2,000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for IgA antibody

  1. Wyszyśska A et al. (2004) Oral immunization of chickens with avirulent Salmonella vaccine strain carrying C. jejuni 72Dz/92 cjaA gene elicits specific humoral immune response associated with protection against challenge with wild-type Campylobacter.
    Vaccine. 22 (11-12): 1379-89.
  2. Beal, R.K. et al. (2004) Age at primary infection with Salmonella enterica serovar Typhimurium in the chicken influences persistence of infection and subsequent immunity to re-challenge.
    Vet Immunol Immunopathol. 100 (3-4): 151-64.
  3. Beal, R.K. et al. (2004) Temporal dynamics of the cellular, humoral and cytokine responses in chickens during primary and secondary infection with Salmonella enterica serovar Typhimurium.
    Avian Pathol. 33 (1): 25-33.
  4. Barrow, P.A. et al. (2004) Faecal shedding and intestinal colonization of Salmonella enterica in in-bred chickens: the effect of host-genetic background.
    Epidemiol Infect. 132 (1): 117-26.
  5. Withanage, G.S. et al. (2005) Cytokine and chemokine responses associated with clearance of a primary Salmonella enterica serovar Typhimurium infection in the chicken and in protective immunity to rechallenge.
    Infect Immun. 73 (8): 5173-82.
  6. Beal, R.K. et al. (2005) A strong antigen-specific T-cell response is associated with age and genetically dependent resistance to avian enteric salmonellosis.
    Infect Immun. 73: 7509-16.
  7. Rezar, V. et al. (2007) Dose-dependent effects of T-2 toxin on performance, lipid peroxidation, and genotoxicity in broiler chickens.
    Poult Sci. 86 (6): 1155-60.
  8. Zhang L et al. (2008) Enhancement of mucosal immune responses by intranasal co-delivery of Newcastle disease vaccine plus CpG oligonucleotide in SPF chickens in vivo.
    Res Vet Sci. 85 (3): 495-502.
  9. View The Latest Product References
  10. Singh, R. (2010) Immunogenicity and protective efficacy of virosome based vaccines against Newcastle disease.
    Trop Anim Health Prod. 42: 465-71
  11. Buckley, A.M. et al. (2010) Evaluation of live-attenuated Salmonella vaccines expressing Campylobacter antigens for control of C. jejuni in poultry.
    Vaccine. 28: 1094-105.
  12. Park, S.I. et al. (2010) Immune response induced by ppGpp-defective Salmonella enterica serovar Gallinarum in chickens.
    J Microbiol. 48 (5): 674-81.
  13. Koppad, S. et al. (2011) Calcium phosphate coupled Newcastle disease vaccine elicits humoral and cell mediated immune responses in chickens.
    Res Vet Sci. 91 (3): 384-90.
  14. Andersen, J.P. et al. (2013) No protection in chickens immunized by the oral or intra-muscular immunization route with Ascaridia galli soluble antigen.
    Avian Pathol. 42 (3): 276-82.
  15. Salisbury Anne-Marie et al. (2014) Salmonella Virchow Infection of the Chicken Elicits Cellular and Humoral Systemic and Mucosal Responses, but Limited Protection to Homologous or Heterologous Re-Challenge
    Frontiers in Veterinary Science. 1: 6.
  16. Barman, N.N. et al. (2014) Reflection of serum immunoglobulin isotypes in the egg yolk of laying hens immunized with enterotoxigenic Escherichia coli
    Veterinary World. 7 (9): 749-53.
  17. Park, E.H. et al. (2014) Protective efficacy of a single dose of baculovirus hemagglutinin-based vaccine in chickens and ducks against homologous and heterologous H5N1 virus infections.
    Viral Immunol. 27 (9): 449-62.
  18. Sadeyen JR et al. (2014) Analysis of immune responses induced by avian pathogenic Escherichia coli infection in turkeys and their association with resistance to homologous re-challenge.
    Vet Res. 45: 19.
  19. Bérto Letícia Dal et al. (2015) Live and Inactivated Salmonella enteritidis Vaccines: Immune Mechanisms in Broiler Breeders
    World Journal of Vaccines. 05 (04): 155-164.
  20. Radomska, K.A. et al. (2016) Chicken Immune Response after In Ovo Immunization with Chimeric TLR5 Activating Flagellin of Campylobacter jejuni.
    PLoS One. 11 (10): e0164837.
  21. Beir˜o, B.C.B. et al. (2018) Effect of an Enterococcus faecium. probiotic on specific IgA following live Salmonella enteritidis. vaccination of layer chickens.
    Avian Pathol. 47 (3): 325-33.
  22. Al-Karagoly, H. et al. (2019) Turkey humoral and cell-mediated immune responses to a Newcastle viscerotropic vaccine and its association with major histocompatibility complex.
    Bulg J Vet Med. 22 (1): 26-40.
  23. Bonato, M. et al. (2020) Effects of yeast cell wall on immunity, microbiota, and intestinal integrity of Salmonella-infected broilers
    Journal of Applied Poultry Research. 29 (3): 545-58.
  24. Śmiałek, M. et al. (2021) The influence of maternally derived antibodies on protection against aMPV/A infection in TRT vaccinated turkeys.
    Poult Sci. 100 (5): 101086.




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