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IgG antibody

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Goat anti Cat IgG (Fc):FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG
Region
(Fc)

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Goat anti cat IgG (Fc) antibody recognizes feline IgG (Fc) and shows no cross-reactivity with other feline immunoglobulin classes in immunoelectrophoresis.

Target Species
Cat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Iberian Lynx
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to feline IgG were raised by repeated immunisation of goat with highly purified antigen. Purified IgG prepared by affinity chromatography using antigen coupled to agarose beads.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Immunogen
Purified feline IgG (Fc) fragment.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/50 1/500
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.

References for IgG antibody

  1. Norris, C.R. et al. (2003) Production of polyclonal antisera against feline immunoglobulin E and its use in an ELISA in cats with experimentally induced asthma.
    Vet Immunol Immunopathol. 96: 149-57.
  2. Jiménez, A. et al. (2008) Membranous glomerulonephritis in the Iberian lynx (Lynx pardinus).
    Vet Immunol Immunopathol. 121: 34-43.
  3. Saari, S. et al. (2008) A nonhealing ulcerative skin lesion associated with Trichinella nativa infection in a cat.
    J Vet Diagn Invest. 20:839-43.
  4. De Craeye, S. et al. (2008) Prevalence of Toxoplasma gondii infection in Belgian house cats.
    Vet Parasitol. 157:128-32.
  5. Tochikura, T.S. et al. (2010) Differential CXCR4 expression and function in subpopulations of the feline lymphoma cell line 3201 susceptible to feline immunodeficiency virus.
    J Feline Med Surg. 12: 269-77.
  6. Alvarez-Guerrero C et al. (2014) Pathological and parasitological traits in experimentally infected cats with Gnathostoma binucleatum (Spirurida: Gnathostomatidae).
    Vet Parasitol. 204 (3-4): 279-84.
  7. Persichetti, M.F. et al. (2017) Diagnostic performance of ELISA, IFAT and Western blot for the detection of anti-Leishmania infantum antibodies in cats using a Bayesian analysis without a gold standard.
    Parasit Vectors. 10 (1): 119.
  8. Pereira, A. et al. (2019) Antibody response to Phlebotomus perniciosus saliva in cats naturally exposed to phlebotomine sand flies is positively associated with Leishmania infection.
    Parasit Vectors. 12 (1): 128.
    9. View The Latest Product References
  9. Cherif, A.M. et al. (2022) A cross- sectional study of Leishmania infantum infection in stray cats in Algiers’ suburbs, Algeria, and evaluation of serological and molecular tests for its diagnosis
    Veterinaria 71 (1): 73-83.
  10. Balan, P. et al. (2020) Orally administered ovine serum immunoglobulins modulate dental plaque in cats.
    Res Vet Sci. 133: 262-268.

RRID
AB_323010

AAI26F

151880 162390 167357

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