17-beta-Estradiol antibody
Sheep anti 17-beta estradiol antibody used to assess circulating estradiol levels in the Hylid frog Smilisca sordida by radioimmune assay.
Image caption:
Circulating hormone levels in S. sordida.
Levels of progesterone (P), dihydrotestosterone (DHT), testosterone (T), estradiol (E2) and corticosterone (CORT) in (A) northern and southern latitude populations and (B) high and low elevations. Two asterisks indicate significance with a Benjamini and Hochberg correction and single asterisk indicates significance only when the correction was not applied. Bars represent ±1SE.
From: Leary CJ, Ralicki HF, Laurencio D, Crocker-Buta S, Malone JH (2018)
Assessing the links among environmental contaminants, endocrinology, and parasites to understand amphibian declines in montane regions of Costa Rica.
PLoS ONE 13(1): e0191183.
Sheep anti 17-beta-estradiol polyclonal antibody recognizes 17 β-estradiol, a mammalian estrogenic hormone. 17 β-estradiol is produced in the ovaries, placenta and testis and has a role in normal sexual development and the regulation of the menstrual cycle.The following cross reactivites of Sheep anti 17-beta-estradiol have been observed:
Aldosterone, Cortisol, Corticosterone, DHEA, Deoxycorticosterone, Pregnenolone, Progesterone, 17OH-P and Testosterone all <0.01%
| 17-beta Oestradiol | 100% |
| Oestrone | 14% |
| Oestriol | 5% |
Product Details
- Target Species
- Broad
- Species Cross-Reactivity
-
Target Species Cross Reactivity Sturgeon Anole lizard Sunfish Horned lizard Agamid lizard - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Immunogen
- Estradiol conjugated to bovine serum albumin
- Approx. Protein Concentrations
- 5.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of 17-beta-Estradiol antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/5000 | 1/40,000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rabbit anti Sheep IgG (H/L):Biotin | 5184-2304 | 1.5 ml | C E F WB | ||
| Donkey anti Sheep/Goat IgG:DyLight®488 | STAR88D488GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:DyLight®549 | STAR88D549GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:DyLight®649 | STAR88D649GA | 0.1 mg | F IF | ||
| Donkey anti Sheep/Goat IgG:FITC | STAR88F | 1 mg | C | ||
| Donkey anti Sheep/Goat IgG:HRP | STAR88P | 1 ml | C E P WB |
Product Specific References
References for 17-beta-Estradiol antibody
-
Qu, Q.Z. et al. (2010) The relationships between gonad development and sex steroid levels at different ages in Acipenser schrenckii.
Journal of Applied Ichthyology 26: 1-5. -
Lovern, M.B. and Wade, J. (2003) Sex steroids in green anoles (Anolis carolinensis): uncoupled maternal plasma and yolking follicle concentrations, potential embryonic steroidogenesis, and evolutionary implications.
Gen Comp Endocrinol. 134: 109-15. -
Magee, S.E. et al. (2006) Plasma levels of androgens and cortisol in relation to breeding behavior in parental male bluegill sunfish, Lepomis macrochirus.
Horm Behav. 49: 598-609. -
Warner, D.A. et al. (2007) Maternal nutrition affects reproductive output and sex allocation in a lizard with environmental sex determination.
Proc Biol Sci. 274: 883-90. -
Wack, C.L. et al. (2008) Effects of sex, age, and season on plasma steroids in free-ranging Texas horned lizards (Phrynosoma cornutum).
Gen Comp Endocrinol. 155:589-96. -
Leary, C.J. et al. (2018) Assessing the links among environmental contaminants, endocrinology, and parasites to understand amphibian declines in montane regions of Costa Rica.
PLoS One. 13 (1): e0191183.
