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17-beta-Estradiol antibody

Sheep anti 17-beta-Estradiol

Product Type
Polyclonal Antibody
Polyclonal IgG

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.25 ml loader
List Price Your Price

Sheep anti 17-beta-estradiol polyclonal antibody recognizes 17 β-estradiol, a mammalian estrogenic hormone. 17 β-estradiol is produced in the ovaries, placenta and testis and has a role in normal sexual development and the regulation of the menstrual cycle.

The following cross reactivites of Sheep anti 17-beta-estradiol have been observed:

17-beta Oestradiol 100%

Aldosterone, Cortisol, Corticosterone, DHEA, Deoxycorticosterone, Pregnenolone, Progesterone, 17OH-P and Testosterone all <0.01%

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Anole lizard
Horned lizard
Agamid lizard
Zebra finch
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Estradiol conjugated to bovine serum albumin
Approx. Protein Concentrations
5.0 mg/ml
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/40,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 C E F WB 1.5 ml loader
List Price Your Price
Description Rabbit anti Sheep IgG (H/L):Biotin

References for 17-beta-Estradiol antibody

  1. Qu, Q.Z. et al. (2010) The relationships between gonad development and sex steroid levels at different ages in Acipenser schrenckii.
    J App Ichthyol 26: 1-5.
  2. Lovern, M.B. and Wade, J. (2003) Sex steroids in green anoles (Anolis carolinensis): uncoupled maternal plasma and yolking follicle concentrations, potential embryonic steroidogenesis, and evolutionary implications.
    Gen Comp Endocrinol. 134: 109-15.
  3. Magee, S.E. et al. (2006) Plasma levels of androgens and cortisol in relation to breeding behavior in parental male bluegill sunfish, Lepomis macrochirus.
    Horm Behav. 49: 598-609.
  4. Warner, D.A. et al. (2007) Maternal nutrition affects reproductive output and sex allocation in a lizard with environmental sex determination.
    Proc Biol Sci. 274: 883-90.
  5. Wack, C.L. et al. (2008) Effects of sex, age, and season on plasma steroids in free-ranging Texas horned lizards (Phrynosoma cornutum).
    Gen Comp Endocrinol. 155:589-96.
  6. Lampen, J. et al. (2017) ZENK induction in the zebra finch brain by song: Relationship to hemisphere, rhythm, oestradiol and sex.
    J Neuroendocrinol. 29 (12) e12543.
  7. Leary, C.J. et al. (2018) Assessing the links among environmental contaminants, endocrinology, and parasites to understand amphibian declines in montane regions of Costa Rica.
    PLoS One. 13 (1): e0191183.



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