17-beta-Estradiol antibody

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Sheep anti 17-beta-Estradiol

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7010-2650 E 0.25 ml
Sheep anti 17-beta-estradiol polyclonal antibody recognizes 17 β-estradiol, a mammalian estrogenic hormone. 17 β-estradiol is produced in the ovaries, placenta and testis and has a role in normal sexual development and the regulation of the menstrual cycle.

The following cross reactivites of Sheep anti 17-beta-estradiol have been observed:

17-beta Oestradiol 100%
Oestrone14%
Oestriol5%

Aldosterone, Cortisol, Corticosterone, DHEA, Deoxycorticosterone, Pregnenolone, Progesterone, 17OH-P and Testosterone all <0.01%

Product Details

Target Species
Broad
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sturgeon
Anole lizard
Sunfish
Horned lizard
Agamid lizard
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Estradiol conjugated to bovine serum albumin
Approx. Protein Concentrations
5.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of 17-beta-Estradiol antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/5000 1/40,000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Sheep IgG (H/L):Biotin 5184-2304 1.5 ml C E F WB
Donkey anti Sheep/Goat IgG:DyLight®488 STAR88D488GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®549 STAR88D549GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:DyLight®649 STAR88D649GA 0.1 mg F IF
Donkey anti Sheep/Goat IgG:FITC STAR88F 1 mg C
Donkey anti Sheep/Goat IgG:HRP STAR88P 1 ml C E P WB

Product Specific References

References for 17-beta-Estradiol antibody

  1. Qu, Q.Z. et al. (2010) The relationships between gonad development and sex steroid levels at different ages in Acipenser schrenckii.
    Journal of Applied Ichthyology 26: 1-5.
  2. Lovern, M.B. and Wade, J. (2003) Sex steroids in green anoles (Anolis carolinensis): uncoupled maternal plasma and yolking follicle concentrations, potential embryonic steroidogenesis, and evolutionary implications.
    Gen Comp Endocrinol. 134: 109-15.
  3. Magee, S.E. et al. (2006) Plasma levels of androgens and cortisol in relation to breeding behavior in parental male bluegill sunfish, Lepomis macrochirus.
    Horm Behav. 49: 598-609.
  4. Warner, D.A. et al. (2007) Maternal nutrition affects reproductive output and sex allocation in a lizard with environmental sex determination.
    Proc Biol Sci. 274: 883-90.
  5. Wack, C.L. et al. (2008) Effects of sex, age, and season on plasma steroids in free-ranging Texas horned lizards (Phrynosoma cornutum).
    Gen Comp Endocrinol. 155:589-96.
  6. Leary, C.J. et al. (2018) Assessing the links among environmental contaminants, endocrinology, and parasites to understand amphibian declines in montane regions of Costa Rica.
    PLoS One. 13 (1): e0191183.