Sheep anti 17-beta-estradiol polyclonal antibody
recognizes 17 β-estradiol, a mammalian estrogenic hormone. 17 β-estradiol is produced in the ovaries, placenta and testis and has a role in normal sexual development and the regulation of the menstrual cycle.The following cross reactivites of Sheep anti 17-beta-estradiol have been observed:
|17-beta Oestradiol ||100%|
Aldosterone, Cortisol, Corticosterone, DHEA, Deoxycorticosterone, Pregnenolone, Progesterone, 17OH-P and Testosterone all <0.01%
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Estradiol conjugated to bovine serum albumin
- Approx. Protein Concentrations
- 5.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of 17-beta-Estradiol antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for 17-beta-Estradiol antibody
Qu, Q.Z. et al. (2010) The relationships between gonad development and sex steroid levels at different ages in Acipenser schrenckii.
Journal of Applied Ichthyology 26: 1-5.
Lovern, M.B. and Wade, J. (2003) Sex steroids in green anoles (Anolis carolinensis): uncoupled maternal plasma and yolking follicle concentrations, potential embryonic steroidogenesis, and evolutionary implications.
Gen Comp Endocrinol. 134: 109-15.
Magee, S.E. et al. (2006) Plasma levels of androgens and cortisol in relation to breeding behavior in parental male bluegill sunfish, Lepomis macrochirus.
Horm Behav. 49: 598-609.
Warner, D.A. et al. (2007) Maternal nutrition affects reproductive output and sex allocation in a lizard with environmental sex determination.
Proc Biol Sci. 274: 883-90.
Wack, C.L. et al. (2008) Effects of sex, age, and season on plasma steroids in free-ranging Texas horned lizards (Phrynosoma cornutum).
Gen Comp Endocrinol. 155:589-96.
Leary, C.J. et al. (2018) Assessing the links among environmental contaminants, endocrinology, and parasites to understand amphibian declines in montane regions of Costa Rica.
PLoS One. 13 (1): e0191183.