Tubulin Beta Antibody | TU-13

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  • Mouse anti Pig Tubulin Beta (N-Terminal)
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    TU-13
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2703C, E, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2703
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Pig Tubulin beta antibody, clone TU-13 recognizes an epitope within the N-terminal region (NT) of tubulin beta, a ~50 kDa globular protein and major constituent of microtubules within the cytoskeleton, involved in meiosis, mitosis, intracellular transport and maintaining cell shape.

      The evolutionarily conserved heterodimer tubulin, made up of a tubulin alpha and beta chain, binds two molecules of GTP, one at a non-exchangeable site on the alpha chain and one at an exchangeable site on the beta chain. Only the beta chain possesses GTPase activity however, hydrolysing GTP to GDP, and influencing the stability of the dimer within the microtubule.

      Mouse anti Pig Tubulin beta antibody, clone TU-13 partially inhibits microtubule assembly in vitro (Draber, P. et al.). Mouse anti Pig Tubulin beta antibody, clone TU-13 has also been successfully employed for the demonstration of neuronal cells in mouse brain by immunohistochemistry on floating sections (Nováková et al. 1997).
    • Intended Use
    • Target Species
      Pig
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      Plantsyes
      Mouseyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Tubulin beta from porcine brain.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunohistology - Frozen
      Western Blotting1.0ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2703 detects a band of approximately 50kDa in human RAJI and mouse EL4 cell lysates.
    • Instructions For Use

    Additional Tubulin Beta Antibody Formats

    Formats Clone Applications Sizes available
    Tubulin Beta Antibody : Purified TU-13 C, E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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