Create mode – the default mode when you create a requisition and PunchOut to Bio-Rad. You can create and edit multiple shopping carts
Edit mode – allows you to edit or modify an existing requisition (prior to submitting). You will be able to modify only the cart that you have PunchedOut to, and won't have access to any other carts
Inspect mode – when you PunchOut to Bio-Rad from a previously created requisition but without initiating an Edit session, you will be in this mode. You cannot modify any Cart contents
Bio-Rad-Antibodies.com relies on third-party cookies to show you pricing, allow you to order online, and connect you to My Bio-Rad. Please amend your browser settings to enable third-party cookies and access this website’s full functionality.
The following tables show that the level of infection, cell type and mycoplasma strains may influence specific results. We encourage researchers to use this data as a helpful guide to indicate the effective Mycoplasma Removal Agent concentration needed with their specific cell line and mycoplasma strain.
Mycoplasma Removal Effect – Spontaneous Infection |
|||||
---|---|---|---|---|---|
Concentration of MRA | (ug/ml) | Days* | |||
0 | 7 | 14 | 21 | ||
Human-derived cell-A | 0.39 | + | - | - | - |
0.2 | + | - | - | - | |
0.1 | + | - | + | + | |
0 | + | + | + | + | |
Human-derived cell-B | 0.78 | + | - | - | - |
0.39 | + | - | - | - | |
0.2 | + | - | - | - | |
0.1 | + | + | + | + | |
0 | + | + | + | + |
(+) = Mycoplasma positive, (-) = Mycoplasma negative, (Days*) = Mycoplasma status at stage of culture (days).
Comparisons with other antibiotics on the market such as Tiamulin and Minocycline shows that Mycoplasma Removal Agent is comparable for its minimum inhibitory concentration (MIC), with MICs from as low as 0.1 µg/ml for all the tested bacteria (see Table 2). This shows that if you are looking to inhibit the growth of Mycobacteria within your cultures, you can reliably use Mycoplasma Removal Agent because it is just as effective as other antibiotics which target mycobacteria.
Where Mycoplasma Removal Agent out performs the other tested antibiotics is in its low Minimum Microbicidal Concentration (MMC) which shows consistently low concentrations required for microbicidal effect (see Table 2). This enables you to confidently kill and remove mycoplasma from cultures at low concentrations of antibiotic. This reduces the chance that the antibiotic will have any inhibitory effects upon other cells in your culture.
Efficacy Comparison of MRA and Pharmaceuticals on the Market |
||||||
---|---|---|---|---|---|---|
MRA | Tiamulin | Minocycline | ||||
MIC* | MMC** | MIC | MMC | MIC | MMC | |
Mycoplasma orale CH-19299 | 0.05 | 0.1 | 0.0031 | 3.13 | 0.05 | 25.0 |
Mycoplasma arginini G-230
|
0.1 | 0.2 | 0.0063 | 12.5 | 0.2 | (>)100 |
Mycoplasma hyorhinis BST-7 | 0.05 | 0.1 | 0.0031 | 0.39 | 0.0031 | 0.39 |
Acholeplasma laidlawii PG-8> | 0.0125 | 0.025 | 0.05 | (>)100 | 0.05 | (>)100 |
MMC/MIC | 2 | 128 - (>) 2048 | 512 - (>) 2048 |
*MIC = Minimum inhibitory concentration (µg/ml), **MMC = Minimum microbicidal concentration (µg/ml)