RIP3 Antibody

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RIP3 Antibody gallery image 1

Western blot analysis of whole cell lysate from mouse 3T3 cells probed with Rabbit anti RIP3 (AHP1797) in the absence (A) or presence (B) of blocking peptide

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RIP3 Antibody gallery image 2

Immunohistochemical staining of rat kidney with Rabbit anti RIP3 (AHP1797)

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  • RIP3 Antibody thumbnail image 1
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  • Rabbit anti RIP3
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1797P *, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP1797
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti RIP3 antibody recognizes mouse serine-threonine kinase receptor-interacting protein 3 (RIP3). RIP3 is related to RIP and RIP2 but does not posses a death domain or CARD motif at its C-terminus. RIP3 activates NF-Kappa B and potently induces apoptosis.

      RIP3 is expressed in a range of tissues including the spleen, liver, testis, heart, brain and lung.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to mouse RIP3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide sequence corresponding to amino acids 473-486 of mouse RIP3.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      NF-kappaB-inducing kinase activity
      induction of apoptosis
      apoptosis
      ATP binding
      I-kappaB kinase/NF-kappaB cascade
      protein binding
      cytoplasm
      protein phosphorylation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.51.0ug/ml
      Immunohistology - Paraffin(1)5ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Rat kidney
    • Immunofluorescence
    • Western Blotting
      AHP1797 detects a band of approximately 56 kDa in Mouse 3T3 whole cell lysate
    • Instructions For Use

    Additional RIP3 Antibody Formats

    Formats Applications Sizes available
    RIP3 Antibody : Purified P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000A50 TestsC, P
          STAR3000A
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Rat kidney
              • Application NameReference Images
                Immunofluorescence
                Western Blotting

              References

              Further Reading

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