Recombinant mouse IL-7 has an ED50 of 0.2ng/ml, determined in a dose response proliferation assay of murine 2E8 cells.
Purified cytokine from E. coli - lyophilised
Reconstitute with 0.1 ml distilled water. For extended storage it is recommended to further dilute in a buffer containing 0.1% BSA. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently vortexed after reconstitution and microcentrifuged before use.
Recombinant IL-7 is purified by sequential chromatography
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
Protein Molecular Weight
15kDa (129 Amino acid sequence)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.