IL-10 has a range of biological properties, including inhibition of macrophage mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response.
ED50 is <2.0ng/ml, as determined by the dose dependent co-stimulation of MC/9 cells (with murine IL-4).
Purified recombinant protein expressed in E. coli - lyophilised
Reconstitute with 0.1ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
5 x 105 units/mg
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
0.1mg/ml after reconstitution
Protein Molecular Weight
18.7 kD (161 amino acid sequence)
Reagents In The Kit
Preparing The Antibody
5mM Sodium Phosphate
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the protein for use in their own system using appropriate positive/negative controls.
PMP21 may be used as a standard for ELISA applications with AAM32
Histology Positive Control Tissue
PMP21 may be used as a positive control for Western blotting applications with AAM32