IFN Gamma

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Published customer image:
Murine macrophages cultured in the presence of mouse interferon-γ (PMP43A) or mouse interleukin-4 (PMP81 ) to generate an M1 and M2 phenotype respectively. cells were sublequently exposed to bacteria as indicated to visualise phagocytosis.
Image caption:
3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages.
(A,B,C) Maximum intensity projection and (D,E,F) the corresponding slice view 3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages (60:1 BMR, 1 hour exposure time), respectively, on the Deltavision OMX Structured Illumination Microscope V4 Blaze (Applied Precision, WA, USA). P. gingivalis W50 were labelled with AF488 (green) while the nuclei and actin filaments of the macrophages were stained with DAPI (blue) and Phalloidin-TRITC (red) respectively. The slice images were obtained from a section with defined size (between 10–50 z-stack) to confirm phagocytosis of P. gingivalis W50 by naive, M1 and M2 macrophages.

From: Lam RS, O'Brien-Simpson NM, Holden JA, Lenzo JC, Fong SB, Reynolds EC (2016)
Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
PLoS ONE 11(7): e0158629.

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  • Recombinant Mouse Interferon Gamma
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  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PMP43AE, FN, WBdatasheet pdfdatasheet pdf20 µg
    PMP43A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant mouse interferon gamma is an E. coli-derived recombinant protein corresponding to full-length mature murine interferon gamma, a regulatory cytokine, which is produced by activated natural killer cells and certain T-lymphocytes.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.20.4ng/well
      Functional Assays0.11.0ng/ml
      Western Blotting1.53.0ng/lane

    • Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PMP43A may be used as a standard in ELISA applications with AAM29
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      PMP43A may be used as a positive control in Western blotting applications with AAM29
    • Instructions For Use

    Additional IFN Gamma Formats

    Formats Applications Sizes available
    IFN Gamma : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti Mouse Interferon GammaAAM290.1 mgE, FN, WB
          AAM29

          Recommended Positive Controls

            Histology Controls

              References

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