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CD150 antibody | 9D1

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Anti Mouse CD150 Antibody, clone 9D1 thumbnail image 1
Anti Mouse CD150 Antibody, clone 9D1 gallery image 1
Figure A. PE-A750 tandem-conjugated Rat anti Mouse CD45R (MCA1258P750) and RPE conjugated Rat IgG1 isotype control (MCA6004PE). Figure B. PE-A750 tandem-conjugated Rat anti Mouse CD45R (MCA1258P750) and RPE conjugated Rat anti Mouse CD150 (MCA2274PE). All experiments performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A).

Rat anti Mouse CD150:RPE

Product Type
Monoclonal Antibody
Clone
9D1
Isotype
IgG1
Specificity
CD150

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Product Code Applications Pack Size List Price Your Price Qty
MCA2274PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Rat anti Mouse CD150 antibody, clone 9D1 recognizes murine CD150, also known as signaling lymphocyte activation molecule (SLAM) . Murine CD150 is a 75 kDa cell surface glycoprotein that is expressed on T-cells and B-cells, and is upregulated on activated T-cells and stimulated macrophages. The extracellular domain of CD150 is the receptor for the measles virus (Erlenhoefer et al. 2001) and acts as a co-activator on T-cells and B-cells.

Rat anti Mouse CD150 antibody, clone 9D1 has functional activity (Howie et al. 2002).

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
5% sucrose
Immunogen
CHO cells stably transfected with mouse CD150
Fusion Partners
Spleen cells from immunised rats were fused with cells of a myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4°C. Following reconstitution store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for CD150 antibody

  1. Howie, D. et al. (2002) The role of SAP in murine CD150 (SLAM)-mediated T-cell proliferation and interferon gamma production.
    Blood. 100 (8): 2899-907.
  2. Desponts, C. et al. (2006) SHIP deficiency enhances HSC proliferation and survival but compromises homing and repopulation.
    Blood. 107: 4338-45.
  3. Almolda, B. et al. (2015) Alterations in microglial phenotype and hippocampal neuronal function in transgenic mice with astrocyte-targeted production of interleukin-10.
    Brain Behav Immun. 45: 80-97.
  4. Villacampa, N. et al. (2015) Astrocyte-targeted production of IL-10 induces changes in microglial reactivity and reduces motor neuron death after facial nerve axotomy.
    Glia. 63 (7): 1166-84.

Synonyms
SLAM
RRID
AB_566533
UniProt
Q9QUM4
Entrez Gene
Slamf1
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0009897 external side of plasma membrane
GO:0046649 lymphocyte activation

MCA2274PE

154619

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