V5-Tag antibody | SV5-Pk5
Mouse anti V5-Tag:DyLight®550
- Product Type
- Monoclonal Antibody
- Clone
- SV5-Pk5
- Isotype
- IgG1
- Specificity
- V5-Tag
Mouse anti V5-Tag antibody, clone SV5-Pk5 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk5 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined). |
- Target Species
- Viral
- Product Form
- Purified IgG conjugated to DyLight®550 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Dylight®550 562 576 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is manufactued under an exclusive license from the University of St. Andrews, UK.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/5 | |
Immunofluorescence | |||
Western Blotting | 1/1,000 | 1/25,000 |
References for V5-Tag antibody
-
Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.
J Immunol Methods. 224 (1-2): 141-50. -
Hsu, W.L. et al. (2023) Identification of Ndfip1 as a novel negative regulator for spatial memory formation associated with increased ubiquitination of Beclin 1 and PTEN.
PLoS One. 18 (4): e0283908.
- Synonyms
- PK-TAG
MCA2895D550
152523If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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