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MHC Class II DR Monomorphic antibody | 37.68

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Anti Sheep MHC Class II DR Monomorphic Antibody, clone 37.68 thumbnail image 1 Anti Sheep MHC Class II DR Monomorphic Antibody, clone 37.68 thumbnail image 2
Anti Sheep MHC Class II DR Monomorphic Antibody, clone 37.68 gallery image 1
Published customer image:
FITC conjugated Mouse anti Sheep MHC class II antibody, clone 37.68 (MCA2226F) used to evaluate MHC class II expression on goat monocytes by flow cytometry.
Image caption:
rFg-CaBP4-mediated differential expression of major histocompatibility complex (MHC) on the surface of goat monocytes. Monocytes were cultured in the presence of the indicated concentrations of rFg-CaBP4 and control buffer for 24 h. MHC class-I (MHC-I) and MHC-II expressions were measured by flow cytometric analysis and calculated as the percentage of mean fluorescence intensity (MFI). The data are presented as the MFI ± SD from three independent experiments. **P <0.01, ***P <0.001, ****P <0.0001, ns (compared to untreated control)

From: Ehsan M, Hu RS, Hou JL, Elsheikha HM, Li XD, Liang PH, Zhu XQ.
Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
Parasit Vectors. 2021 May 22;14(1):276.
doi: 10.1186/s13071-021-04784-5.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Sheep MHC Class II DR Monomorphic Antibody, clone 37.68 gallery image 2
Published customer image:
Fitc conjugated Mouse anti Sheep MHC Class II antibody, clone 37.68 (MCA2226F) used to evaluate MHC Class II expression in goat monocytes by flow cytometry.
Image caption:
Inhibitory effect of recombinant rhodanese protein from Haemonchus contortus (rHCRD) on the expression of MHC-II by goat monocytes. Cells were cultured in the presence of varies rHCRD concentrations and his-tagged protein or control buffer (PBS/DTT) for 24 h. The cells treated with LPS were used as positive control. MHC-II expression was analysed on flow cytometric analysis and calculated as the percentage of mean fluorescence intensity (MFI) of controls. Bars represent the MFI ± SD of controls. The data presented are results of three independent experiments (*P <0.05, **P <0.01, ***P <0.001). a MHC-I. b MHC-II

From: Wang Y, Ehsan M, Huang J, Aimulajiang K, Yan R, Song X, Xu L, Li X.
Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
Parasit Vectors. 2020 Sep 7;13(1):454.
doi: 10.1186/s13071-020-04333-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Sheep MHC Class II DR Monomorphic:FITC

Product Type
Monoclonal Antibody
Clone
37.68
Isotype
IgG2a
Specificity
MHC Class II DR Monomorphic

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Product Code Applications Pack Size List Price Your Price Qty
MCA2226F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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Mouse anti Sheep MHC class II antibody, clone 37.68 recognizes a monomorphic epitope on ovine MHC class II DR molecules, constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Goat
Human
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Splenocytes from ATL mice.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
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Description Mouse IgG2a Negative Control:FITC

Source Reference

  1. Puri, N.K. et al. (1985) Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules.
    Immunology. 56 (4): 725-33.

References for MHC Class II DR Monomorphic antibody

  1. Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
    Vet Immunol Immunopathol. 15 (1-2): 59-86.
  2. Puri, N.K. & Brandon, M.R. (1987) Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules.
    Immunology. 62 (4): 575-80.
  3. Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products.
    Hum Immunol. 20 (3): 195-207.
  4. Ballingall, K.T. et al. (1995) Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants.
    Anim Genet. 26 (2): 79-84.
  5. Wang, Y. et al. (2017) Characterization of a secreted cystatin of the parasitic nematode Haemonchus contortus and its immune-modulatory effect on goat monocytes.
    Parasit Vectors. 10 (1): 425.
  6. Wang, Y. et al. (2020) Characterization of a rhodanese homologue from Haemonchus contortus and its immune-modulatory effects on goat immune cells in vitro.
    Parasit Vectors. 13 (1): 454.
  7. López-Fernández, A. et al. (2020) Effect of Allogeneic Cell-Based Tissue-Engineered Treatments in a Sheep Osteonecrosis Model.
    Tissue Eng Part A. 26 (17-18): 993-1004.
  8. Ehsan, M. et al. (2021) Fasciola gigantica. tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.
    Parasit Vectors. 14 (1): 276.
    9. View The Latest Product References

RRID
AB_323966

MCA2226F

0612R 157535 167458

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