MHC Class II DQ DR Polymorphic antibody | 49.1
Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunized against Foot and Mouth Disease Virus by immunofluorescence.
Image caption:
Primary infection of nasopharyngeal mucosal epithelial cells is similar in non-vaccinated and vaccinated cattle.
Microscopic distribution of FMDV structural (VP1) and non-structural (3D) protein in dorsal nasopharyngeal mucosa of non-vaccinated (A-C) and vaccinated (D-F) cattle at 24 hpi. Multichannel immunofluorescent technique. A) Nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). FMDV VP1 (red) and 3D (aqua) proteins co-localize with cytokeratin (green) in foci of primary FMDV infection in superficial layer of follicle-associated epithelium. Scarce MHC II+ cells (purple) are present in the subepithelium. 20x magnification, scale bar 50μm. B-C) 40x magnification of region identified in (A). B) Select channel combinations demonstrating co-localization of FMDV VP1(red) and FMDV 3D (aqua) with cytokeratin (green), but not with MHC II (purple) C) Merge of all images shown in (B), scale bar 25μm. D) Nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). FMDV VP1 (red) and 3D (aqua) proteins co-localize with cytokeratin (green) within follicle associated epithelium. Intra-epithelial MHC II+ cells (purple) are in close proximity to virus-infected epithelial cells. 20x magnification, scale bar 50μm. E-F) 40x magnification of region identified in (D). E) Select channel combinations demonstrating FMDV VP1 (red) and 3D (aqua) colocalization with cytokeratin (green)-expressing cells and with MHC II- expressing cells (purple). E) Merge of images shown in (F), scale bar 25μm.
From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015)
Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle. PLoS ONE 10(11): e0143666..
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 (MCA2228F) used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunised against Foot and Mouth Disease Virus by immunofluorescence.
Image caption:
Differential host-virus interactions in nasopharyngeal mucosa of non-vaccinated (A-C) versus vaccinated (D-F) cattle. Multichannel immunofluorescent technique. A). FMDV VP1 (red) protein within cytokeratin+ cells (green) in epithelial crypt of the nasopharyngeal mucosa of non-vaccinated steer at 24 hpi (animal number 3). Host cells expressing MHC II (purple) and/or CD11c (aqua) are present in the subepithelial compartment and interspersed within epithelium, but without co-localizing with viral protein. 10x magnification, scale bar 100μm. B-C) 40x magnification of region identified in (A), scale bars 25μm. B) Select channel views demonstrate lack of co-localization of FMDV VP1 (red) with CD11c (aqua)/ MHC II (purple)-double positive cells. C) Merge of all images shown in (B), scale bar 25 μm. D) FMDV VP1 (red) protein co-localizes with cytokeratin (green) in an epithelial erosion in dome region of follicle-associated epithelium of nasopharyngeal mucosa of vaccinated steer at 24 hpi (animal number 11). Regionally extensive infiltration by MHC II (purple) and/or CD11c (aqua)-expressing cells co-localizing and interspersing with viral antigen.10x magnification, scale bar 100μm. E-F) 40x magnification of region identified in (D), scale bar 25μm. E) Select channels demonstrating co-localization of FMDV VP1 (red) with CD11c (aqua), MHC II (purple). F) Merge of images shown in (E), scale bar 25 μm.
From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015)
Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
PLoS ONE 10(11): e0143666.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
FITC conjugated Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 (MCA2228F) used for the detection of MHC Class II expressing cells in the nasopharynx of cattle immunized against Foot and Mouth Disease Virus by immunofluorescence.
Image caption:
FMDV co-localizes with cytokeratin and MHCII/CD11c-expressing cells in palatine tonsil of non-vaccinated steer at 72hpi.
A) FMDV VP1 (red) predominantly in cytokeratin-positive cells of epithelial crypt of palatine tonsil of non-vaccinated steer (animal number 9) during viremia and clinical FMD at 72 hpi. Abundant cells expressing MHC II (purple) and/or CD11c (aqua) interspersed with epithelial cells. 10x magnification, scale bar 100 μm. B) Select channels of image in (A). FMDV VP1 (red), cytokeratin (green), CD11c (aqua), MHC II (purple) demonstrate FMDV antigen is exclusively within epithelial compartment. C) High magnification of region identified in (A). Co-localization with FMDV VP1within subset of MHC II (purple) and/or CD11c (aqua) expressing cells within crypt epithelium (arrows in C & D). 40x magnification, scale bar 25 μm D) Individual channels of image in (B). FMDV VP1 (red), cytokeratin (green), CD11c (aqua), MHC II (purple).
From: Stenfeldt C, Eschbaumer M, Pacheco JM, Rekant SI, Rodriguez LL, Arzt J (2015)
Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
PLoS ONE 10(11): e0143666.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Sheep MHC Class II DQ DR Polymorphic:FITC
- Product Type
- Monoclonal Antibody
- Clone
- 49.1
- Isotype
- IgG2a
- Specificity
- MHC Class II DQ DR Polymorphic
Antibody Quality and Performance Guarantee
| Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 recognizes a polymorphic epitope on ovine MHC class II DQ and DR molecules, constitutively expressed on antigen presenting cells including dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma. The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infection. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain. Mouse anti Sheep MHC class II Dq DR antibody, clone 49.1 recognizes ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1 (Ballingall et al. 1995). |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Goat Human Bovine - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1 x 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
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| Description | Mouse IgG2a Negative Control:FITC | ||||||
References for MHC Class II DQ DR Polymorphic antibody
-
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
Vet Immunol Immunopathol. 15 (1-2): 59-86. -
Puri, N.K. & Brandon, M.R. (1987) Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules.
Immunology. 62 (4): 575-80. -
Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products.
Hum Immunol. 20 (3): 195-207. -
Ballingall, K.T. et al. (1995) Analysis of the fine specificities of sheep major histocompatibility complex class II-specific monoclonal antibodies using mouse L-cell transfectants.
Anim Genet. 26 (2): 79-84. -
Kallapur, S.G. et al. (2011) Pulmonary and systemic inflammatory responses to intra-amniotic IL-1α in fetal sheep.
Am J Physiol Lung Cell Mol Physiol. 301 (3): L285-95. -
Gorrell, M.D. et al. (1988) Lymphocyte phenotypes in the intestinal mucosa of sheep infected with Trichostrongylus colubriformis.
Clin Exp Immunol. 72 (2): 274-9. -
Stenfeldt, C. et al. (2015) Clinical and virological dynamics of a serotype O 2010 South East Asia lineage foot-and-mouth disease virus in sheep using natural and simulated natural inoculation and exposure systems.
Vet Microbiol. 178 (1-2): 50-60. -
Stenfeldt, C. et al. (2015) Pathogenesis of Primary Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Vaccinated and Non-Vaccinated Cattle.
PLoS One. 10 (11): e0143666.
View The Latest Product References
-
Stenfeldt, C. et al. (2016) The Foot-and-Mouth Disease Carrier State Divergence in Cattle.
J Virol. 90 (14): 6344-64. -
Stenfeldt, C. et al. (2019) Virulence beneath the fleece; a tale of foot-and-mouth disease virus pathogenesis in sheep.
PLoS One. 14 (12): e0227061.
- RRID
- AB_567329
MCA2228F
1606
164595
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