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MHC Class II DQ DR Polymorphic antibody | 28.1

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Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 thumbnail image 1 Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 thumbnail image 2 Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 thumbnail image 3 Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 thumbnail image 4 Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 thumbnail image 5 Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 thumbnail image 6
Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 gallery image 1
Staining of sheep peripheral blood lymphocytes with FITC conjugated Mouse anti Ovine MHC Class II DQ/DR antibody, clone 28.1 (MCA2225F).
Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 gallery image 2
Staining of sheep peripheral blood lymphocytes with phycoerythrin conjugated Mouse anti Ovine MHC Class II DQ/DR antibody, clone 28.1 (MCA2225PE).
Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 gallery image 3
Published customer image:
Phycoerythrin conjugated Mouse anti Sheep MHC Class II DQ DR antibody, clone 28.1 (MCA2225PE) used for the identification of MHC class II expressing cells in bovine nasopharyngeal tissue sections by immunofluorescence.
Image caption:
Immunofluorescent detection of persistent FMDV in nasopharyngeal mucosa. Dorsal nasopharynx, steer #626, 37dpe, FMDV O1 Manisa. Immunomicroscopy, A) Low magnification (10X) view of an epithelial invagination with a focal cluster of FMDV-antigen-positive cells within superficial epithelial surface, scale bar 50μm. B-F) Higher magnification (40X) views of region of interest identified in A (dashed box). Cells containing FMDV-VP1 are in the superficial epithelium and are cytokeratin-positive. Few MHC-II and CD11c positive cells are present within and below epithelium, but do not contain FMDV-VP1. Indirect fluorescence technique with differential interference contrast, antibody labels color-coded in bottom panel, asterisks indicate channels present in each panel, scale bar 25 μm.

From: Pacheco JM, Smoliga GR, O'Donnell V, Brito BP, Stenfeldt C, Rodriguez LL, et al. (2015)
Persistent Foot-and-Mouth Disease Virus Infection in the Nasopharynx of Cattle; Tissue-Specific Distribution and Local Cytokine Expression.
PLoS ONE 10(5): e0125698.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 gallery image 4
Published customer image:
Phycoerythrin conjugated Mouse anti Ovine MHC Class II antibody, clone 28.1 (MCA2225PE) used to evaluate MHC class II expression in palatine tonsil of FMDV infected cattle by immunofluorescence on cryostat tissue sections.
Image caption:
FMDV generates microvesicles within the palatine tonsil of cattle during the clinical phase of disease. a Low magnification image demonstrates the architecture of a large tonsillar crypt delineated by cytokeratin-positive (green) squamous epithelium. Dashed box indicates region of interest (ROI) within crypt wall containing a focus of FMDV (red)-infected cells forming a microvesicle. ROI is featured at higher magnification in 6b-d including different detection channels in each panel. b Inclusion of FMDV VP1 (red) and cytokeratin (green) channels demonstrates disruption of epithelial architecture with cavitation/vesiculation. Many of the FMDV-containing cells are also cytokeratin-positive (epithelial cells). c Inclusion of FMDV VP1 (red), CD11c (turquoise), and MHC II (purple) demonstrates that some of the FMDV-containing cells are also individually- or double-positive for these markers of monocytoid, phagocytic, and antigen presenting cells. d Simultaneous viewing of all 4 channels demonstrates that within the vesicular cavity, FMDV-containing cells of distinct phenotypes are interspersed and in close proximity. Multichannel immunofluorescence microcopy. (animal ID 938, tissue ID palatine tonsil) (magnification: a 4×, b-d 40×).

From: Arzt J, Pacheco JM, Stenfeldt C, Rodriguez LL.
Pathogenesis of virulent andattenuated foot-and-mouth disease virus in cattle.
Virol J. 2017 May 2;14(1):89.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 gallery image 5
Published customer image:
Phycoerythrin conjugated Mouse anti Ovine MHC Class II antibody, clone 28.1 (MCA2225PE) used to evaluate MHC class II expression in palatine tonsil of FMDV infected cattle by immunofluorescence on cryostat tissue sections.
Image caption:
FMDV generates microvesicles within the palatine tonsil of cattle during the clinical phase of disease. a Low magnification image demonstrates the architecture of a large tonsillar crypt delineated by cytokeratin-positive (green) squamous epithelium. Dashed box indicates region of interest (ROI) within crypt wall containing a focus of FMDV (red)-infected cells forming a microvesicle. ROI is featured at higher magnification in 6b-d including different detection channels in each panel. b Inclusion of FMDV VP1 (red) and cytokeratin (green) channels demonstrates disruption of epithelial architecture with cavitation/vesiculation. Many of the FMDV-containing cells are also cytokeratin-positive (epithelial cells). c Inclusion of FMDV VP1 (red), CD11c (turquoise), and MHC II (purple) demonstrates that some of the FMDV-containing cells are also individually- or double-positive for these markers of monocytoid, phagocytic, and antigen presenting cells. d Simultaneous viewing of all 4 channels demonstrates that within the vesicular cavity, FMDV-containing cells of distinct phenotypes are interspersed and in close proximity. Multichannel immunofluorescence microcopy. (animal ID 938, tissue ID palatine tonsil) (magnification: a 4×, b-d 40×).

From: Arzt J, Pacheco JM, Stenfeldt C, Rodriguez LL.
Pathogenesis of virulent andattenuated foot-and-mouth disease virus in cattle.
Virol J. 2017 May 2;14(1):89.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Sheep MHC Class II DQ DR Polymorphic Antibody, clone 28.1 gallery image 6
Published customer image:
Phycoerythrin conjugated Mouse anti Ovine MHC Class II antibody, clone 28.1 (MCA2225PE) used to evaluate MHC II expression on CD1b+ ovine lymph cells by flow cytometry.
Image caption:
CD1b+ L-DCs are migratory mature DC capable of endocytic and phagocytic activities. After labelling lymph cells with anti- CD1b mAb (A), CD1b+ L-cells were gated and analysed for the expression of different markers. Data express the median (quartiles) of the percentage of positive cells from 3 sheep (B). Plots of different markers expressed by CD1b+ L-DCs are shown (C). May-Grünwald Giemsa staining of sorted and cytocentrifugated CD1b+ L-DCs (D). Expression of mRNA for CCR7, CD103 and DC-SIGN by sorted CD1b+ L-DCs (1, 3, 5 respectively) and in cDNA control (2, 4, 6 respectively) (E). Uptake of FITC-ovalbumin (FITC-OVA) or GFP-Salmonella by CD1b+ L- DCs. Lymph cells (1×106 cells) were incubated with FITC-OVA for 1h at 4°C or 37°C, or with GFP-Salmonella for 30 min at 37°C, followed by CD1b labelling. CD1b+ L- DC subset was gated on lymph cells and FITC-OVA fluorescence shown on dot plots F and G. Total L-APC were gated on SSC/FSC and the cell population negative for lymphocyte markers. Quadrants were then defined on the CD1b+ and CD1b- subsets and GFP-Salmonella fluorescence shown on dot plot H.

From: Olivier M, Foret B, Le Vern Y, Guilloteau LA (2012)
Capacities of Migrating CD1b+ Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
PLoS ONE 7(1): e30430.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Sheep MHC Class II DQ DR Polymorphic:RPE

Product Type
Monoclonal Antibody
Clone
28.1
Isotype
IgG1
Specificity
MHC Class II DQ DR Polymorphic

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MCA2225PE
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Mouse anti Sheep MHC Class II DQ DR antibody, clone 28.1 recognizes a polymorphic epitope on ovine MHC class II DQ and DR molecules, which are constitutively expressed on antigen presenting cells such as dendritic cells, B lymphocytes, monocytes, macrophages, activated T lymphocytes and may be induced on a range of other cell types by interferon gamma.

The major histocompatibility complex (MHC) is a cluster of genes some of which are important in the immune response to infections. In sheep, this complex is referred to as the ovine leukocyte antigen (OLA) region. There are 2 major types of MHC class IIa molecules encoded by the OLA which are DR and DQ each composed of an alpha and beta chain.

Mouse anti Sheep MHC Class II DQ DR antibody, clone 28.1 recognizes ovine MHC II transfectants DQ - T28.1, DQ - T26.2 and DR - T31.3 but not DR - T8.1. (Ballingall, K. et al. 1995).

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Goat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Ovine alveolar macrophages.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system uising appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
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Description Mouse IgG1 Negative Control:RPE

References for MHC Class II DQ DR Polymorphic antibody

  1. Puri, N.K. et al. (1985) Sheep lymphocyte antigens (OLA). II. Major histocompatibility complex class II molecules.
    Immunology. 56 (4): 725-33.
  2. Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class I and class II molecules: biochemical characterization of three class I gene products and four distinct subpopulations of class II molecules.
    Vet Immunol Immunopathol. 15 (1-2): 59-86.
  3. Puri, N.K. et al. (1987) Sheep MHC class II molecules. I. Immunochemical characterization.
    Immunology. 62 (4): 567-73.
  4. Puri, N.K. & Brandon, M.R. (1987) Sheep MHC class II molecules. II. Identification and characterization of four distinct subsets of sheep MHC class II molecules.
    Immunology. 62 (4): 575-80.
  5. Puri, N.K. et al. (1987) Monoclonal antibodies to sheep MHC class II molecules recognize all HLA-D or subsets of HLA-D region products.
    Hum Immunol. 20 (3): 195-207.
  6. Ferret-Bernard, S. et al. (2011) Mesenteric lymph node cells from neonates present a prominent IL-12 response to CpG oligodeoxynucleotide via an IL-15 feedback loop of amplification.
    Vet Res. 42:19.
  7. Ballingall. K. et al. (1995) Analysis of the fine specificities of sheep major histocompatibility complex class II - Specific monoclonal antibodies using mouse L - Cell transfectants.
    Anim. Genet. 26: 79-84.
  8. Olivier, M. et al. (2012) Capacities of Migrating CD1b Lymph Dendritic Cells to Present Salmonella Antigens to Naive T Cells.
    PLoS One. 7: e30430.
    9. View The Latest Product References
  9. Arzt, J. et al. (2017) Pathogenesis of virulent and attenuated foot-and-mouth disease virus in cattle.
    Virol J. 14 (1): 89.
  10. Ducournau, C. et al. (2020) Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
    Front Immunol. 11: 2183.

Flow Cytometry

RRID
AB_324857
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