CD4 antibody | 44.38

Mouse anti Sheep CD4:Alexa Fluor® 647

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
Search for Batch Specific Datasheets

Mouse anti Sheep CD4 antibody, clone 44.38 recognizes the ovine CD4 cell surface glycoprotein, expressed by a subset of mature T lymphocytes. Mouse anti Sheep CD4 antibody, clone 44.38 immunoprecipitates a protein of ~56 kDa under reducing conditions.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Fetal thymocytes.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS1/1-Ag-4-1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA929A647 F 100 Tests/1ml loader
List Price Your Price
Description Mouse IgG2a Negative Control:Alexa Fluor® 647

References for CD4 antibody

  1. Mackay, C.R. et al. (1986) Three distinct subpopulations of sheep T lymphocytes.
    Eur J Immunol. 16 (1): 19-25.
  2. Mackay, C.R. et al. (1986) Thymocyte subpopulations during early fetal development in sheep.
    J Immunol. 136 (5): 1592-9.
  3. Mackay, C.R. et al. (1987) A monoclonal antibody to the p220 component of sheep LCA identifies B cells and a unique lymphocyte subset.
    Cell Immunol. 110 (1): 46-55.
  4. Brown, M.N. et al. (2010) Chemoattractant receptors and lymphocyte egress from extralymphoid tissue: changing requirements during the course of inflammation.
    J Immunol. 185: 4873-82.
  5. Breugelmans, S. et al. (2010) Immunoassay of lymphocyte subsets in ovine palatine tonsils.
    Acta Histochem. 113: 416-22.
  6. Connelley, T. et al. (2011) NKp46 defines ovine cells that have characteristics corresponding to NK cells.
    Vet Res. 42: 37.
  7. Debes, G.F. et al. (2005) Chemokine receptor CCR7 required for T lymphocyte exit from peripheral tissues.
    Nat Immunol. 6: 889-94.
  8. Foulon, E. et al. (2008) Two populations of ovine bone marrow-derived dendritic cells can be generated with recombinant GM-CSF and separated on CD11b expression.
    J Immunol Methods. 339: 1-10.
  9. View The Latest Product References
  10. Umeshappa, C.S. et al. (2010) Cell-mediated immune response and cross-protective efficacy of binary ethylenimine-inactivated bluetongue virus serotype-1 vaccine in sheep.
    Vaccine. 28: 2522-31.
  11. Silva, A.P. et al. (2015) Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams.
    PLoS One. 10 (8): e0136865.
  12. Gillan, S. et al. (2010) Identification of immune parameters to differentiate disease states among sheep infected with Mycobacterium avium subsp. paratuberculosis.
    Clin Vaccine Immunol. 17: 108-17.
  13. Lacroux, C. et al. (2011) Prionemia and leuco-platelet associated infectivity in sheep TSE models.
    J Virol. 86: 2056-66.
  14. Summers, C. et al. (2012) The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma.
    Vet Immunol Immunopathol. 146: 1-7.
  15. Lybeck, K.R. et al. (2012) Intestinal Strictures, Fibrous Adhesions and High Local Interleukin-10 Levels in Goats Infected Naturally with Mycobacterium avium subsp. paratuberculosis.
    J Comp Pathol. 148: 157-72.
  16. Gómez, D. et al. (2015) Effector T Cell Egress via Afferent Lymph Modulates Local Tissue Inflammation.
    J Immunol. 195 (8): 3531-6.
  17. Kalyanasundaram, A. et al. (2015) Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep.
    Exp Parasitol. 154: 98-107.
  18. Goh, S. et al. (2016) Identification of Theileria lestoquardi Antigens Recognized by CD8+ T Cells.
    PLoS One. 11 (9): e0162571.
  19. Wattegedera, S.R. et al. (2017) Enhancing the toolbox to study IL-17A in cattle and sheep.
    Vet Res. 48 (1): 20.
  20. Greer, A.W. et al. (2018) Immune development and performance characteristics of Romney sheep selected for either resistance or resilience to gastrointestinal nematodes.
    Vet Parasitol. 250: 60-7.
  21. Higgins, J.L. et al. (2018) Cell mediated immune response in goats after experimental challenge with the virulent Brucella melitensis strain 16M and the reduced virulence strain Rev. 1.
    Vet Immunol Immunopathol. 202: 74-84.
  22. Pérez-caballero, R. et al. (2018) Comparative dynamics of peritoneal cell immunophenotypes in sheep during the early and late stages of the infection with Fasciola hepatica by flow cytometric analysis.
    Parasit Vectors. 11 (1): 640.
  23. Baliu-piqué, M. et al. (2019) Age-related distribution and dynamics of T-cells in blood and lymphoid tissues of goats.
    Dev Comp Immunol. 93: 1-10.
  24. Schwarz, E.R. et al. (2020) Experimental Infection of Mid-Gestation Pregnant Female and Intact Male Sheep with Zika Virus.
    Viruses. 12 (3) Mar 07 [Epub ahead of print].
  25. Zhang, H. et al. (2020) Thiamine ameliorates inflammation of the ruminal epithelium of Saanen goats suffering from subacute ruminal acidosis.
    J Dairy Sci. 103 (2): 1931-43.
  26. Ramos, A. et al. (2018) Melatonin enhances responsiveness to Dichelobacter nodosus. vaccine in sheep and increases peripheral blood CD4 T lymphocytes and IgG-expressing B lymphocytes.
    Vet Immunol Immunopathol. 206: 1-8.
  27. Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
    Vet Immunol Immunopathol. 198: 44-53.
  28. Ducournau, C. et al. (2020) Effective Nanoparticle-Based Nasal Vaccine Against Latent and Congenital Toxoplasmosis in Sheep.
    Front Immunol. 11: 2183.
  29. Wooldridge, A.L. et al. (2019) Maternal allergic asthma during pregnancy alters fetal lung and immune development in sheep: potential mechanisms for programming asthma and allergy.
    J Physiol. 597 (16): 4251-62.
  30. Yang, J. et al. (2022) Baseline T-lymphocyte and cytokine indices in sheep peripheral blood.
    BMC Vet Res. 18 (1): 165.
  31. Nashiruddullah, N. et al. (2021) ​Dermal Response to Experimental Orfvirus (ORFV) Infection in Goats, Mice and Rabbit
    Indian J Anim Res. 56 (8): B-4266 1003-9.

Further Reading

  1. Lybeck, K. R. et al. (2009) Neutralization of interleukin-10 from CD14(+) monocytes enhances gamma interferon production in peripheral blood mononuclear cells from Mycobacterium avium subsp. paratuberculosis-infected goats.
    Clin. Vaccine. Immunol. 16: 1003-11.

Flow Cytometry

GO Terms
GO:0016020 membrane
GO:0045058 T cell selection
View more products with CD4 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Sheep Products