CD3 antibody | FN-18

Product Details
- Target Species
- Rhesus Monkey
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Macaque Gorilla Marmoset Cynomolgus monkey African green monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilised
- Reconstitution
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% bovine serum albumin is recommended. - Preparation
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 1% Bovine Serum Albumin
125mM Trehalose - Immunogen
- Rhesus monkey peripheral blood leucocytes.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
Storage Information
- Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
- Regulatory
- For research purposes only
Applications of CD3 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/10 |
- Flow Cytometry
- Use 50ul of the suggested working dilution to label 106 cells in 100ul.
Product Specific References
References for CD3 antibody
-
Nooji, F.J.M. et al. (1986) Polymorphism for RhT3, a CD3-like cell surface antigen, expressed on rhesus monkey T lymphocytes.
Immunology 59: 611. -
Nooij, F. J. et al. (1986) Differentiation antigens on rhesus monkey lymphocytes. II. Characterization of RhT3, a CD3-like antigen on T cells.
Eur. J. Immunol. 16: 981-4. -
Berry, N. et al. (2008) Resistance to superinfection by a vigorously replicating, uncloned stock of simian
immunodeficiency virus (SIVmac251) stimulates replication of a live attenuated
virus vaccine (SIVmacC8).
J Gen Virol. 89: 2240-51. -
Li, B. et al. (2011) Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells.
Retrovirology. 8: 8.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra