CD3 antibody | FN-18
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Mouse anti Monkey CD3:FITC
- Product Type
- Monoclonal Antibody
- Clone
- FN-18
- Isotype
- IgG1
- Specificity
- CD3
- Region
- (POLYMORPHIC)
Antibody Quality and Performance Guarantee
| Mouse anti Monkey CD3 antibody, clone FN-18 recognizes the rhesus monkey RhT3 antigen, the homolog of human CD3. Mouse anti Monkey CD3 antibody, clone FN-18 has been reported to recognize a polymorphic epitope of the RhT3 antigen, which is expressed by mature T lymphocytes. |
- Target Species
- Rhesus Monkey
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Macaque Gorilla Marmoset Cynomolgus monkey African green monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
- Reconstitution
- Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% bovine serum albumin is recommended. - Preparation
- Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 1% bovine serum albumin
125mM Trehalose - Immunogen
- Rhesus monkey peripheral blood leucocytes.
- Fusion Partners
- Spleen cells from immunized mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 50μl of the suggested working dilution to label 106 cells in 100μl
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
References for CD3 antibody
-
Nooji, F.J.M. et al. (1986) Polymorphism for RhT3, a CD3-like cell surface antigen, expressed on rhesus monkey T lymphocytes.
Immunology 59: 611. -
Nooij, F.J. et al. (1986) Differentiation antigens on rhesus monkey lymphocytes. II. Characterization of RhT3, a CD3-like antigen on T cells.
Eur J Immunol. 16 (8): 981-4. -
Berry, N. et al. (2008) Resistance to superinfection by a vigorously replicating, uncloned stock of simian immunodeficiency virus (SIVmac251) stimulates replication of a live attenuated virus vaccine (SIVmacC8).
J Gen Virol. 89: 2240-51. -
Li, B. et al. (2011) Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells.
Retrovirology. 8: 8.
- RRID
- AB_321251
MCA1483F
153154
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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