CD3 antibody | FN-18

Mouse anti Monkey CD3:FITC

Product Type
Monoclonal Antibody
Clone
FN-18
Isotype
IgG1
Specificity
CD3
Region
(POLYMORPHIC)

Product Code Applications Pack Size List Price Your Price Qty
MCA1483F
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Mouse anti Monkey CD3 antibody, clone FN-18 recognizes the rhesus monkey RhT3 antigen, the homolog of human CD3. Mouse anti Monkey CD3 antibody, clone FN-18 has been reported to recognize a polymorphic epitope of the RhT3 antigen, which is expressed by mature T lymphocytes.

Target Species
Rhesus Monkey
Species Cross-Reactivity
Target SpeciesCross Reactivity
Chimpanzee
Macaque
Gorilla
Marmoset
Cynomolgus monkey
African green monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% bovine serum albumin is recommended.
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
1% Bovine Serum Albumin
125mM Trehalose
Immunogen
Rhesus monkey peripheral blood leucocytes.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

References for CD3 antibody

  1. Nooji, F.J.M. et al. (1986) Polymorphism for RhT3, a CD3-like cell surface antigen, expressed on rhesus monkey T lymphocytes.
    Immunology 59: 611.
  2. Nooij, F. J. et al. (1986) Differentiation antigens on rhesus monkey lymphocytes. II. Characterization of RhT3, a CD3-like antigen on T cells.
    Eur. J. Immunol. 16: 981-4.
  3. Berry, N. et al. (2008) Resistance to superinfection by a vigorously replicating, uncloned stock of simian immunodeficiency virus (SIVmac251) stimulates replication of a live attenuated virus vaccine (SIVmacC8).
    J Gen Virol. 89: 2240-51.
  4. Li, B. et al. (2011) Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells.
    Retrovirology. 8: 8.

RRID
AB_321251
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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