CD3 antibody | FN-18

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Mouse anti Monkey CD3:FITC

Product Type: Monoclonal Antibody
Clone: FN-18
Isotype: IgG1
Specificity: CD3
Region: (POLYMORPHIC)

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References (4)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Mouse anti Monkey CD3 antibody, clone FN-18 recognizes the rhesus monkey RhT3 antigen, the homolog of human CD3. Mouse anti Monkey CD3 antibody, clone FN-18 has been reported to recognize a polymorphic epitope of the RhT3 antigen, which is expressed by mature T lymphocytes.

Target Species: Rhesus Monkey
Species Cross-Reactivity: Target Species Cross Reactivity

Chimpanzee

Macaque

Gorilla

Marmoset

Cynomolgus monkey

African green monkey; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilized
Reconstitution: Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% bovine serum albumin is recommended.
Preparation: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 1% bovine serum albumin
125mM Trehalose
Immunogen: Rhesus monkey peripheral blood leucocytes.
Fusion Partners: Spleen cells from immunized mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

FITC 490 525
Regulatory: For research purposes only
Guarantee: Guaranteed until date of expiry. Please see product label.

Target Species	Cross Reactivity
Chimpanzee
Macaque
Gorilla
Marmoset
Cynomolgus monkey
African green monkey

Fluorophore	Excitation Max (nm)	Emission Max (nm)
FITC	490	525

This product is shipped at ambient temperature. Prior to reconstitution store at +4°C.
After reconstitution it is recommended to aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			1/10

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 50μl of the suggested working dilution to label 10⁶ cells in 100μl

References for CD3 antibody

Nooji, F.J.M. et al. (1986) Polymorphism for RhT3, a CD3-like cell surface antigen, expressed on rhesus monkey T lymphocytes.
Immunology 59: 611.
Nooij, F. J. et al. (1986) Differentiation antigens on rhesus monkey lymphocytes. II. Characterization of RhT3, a CD3-like antigen on T cells.
Eur. J. Immunol. 16: 981-4.
Berry, N. et al. (2008) Resistance to superinfection by a vigorously replicating, uncloned stock of simian immunodeficiency virus (SIVmac251) stimulates replication of a live attenuated virus vaccine (SIVmacC8).
J Gen Virol. 89: 2240-51.
Li, B. et al. (2011) Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells.
Retrovirology. 8: 8.