MHC Class I RT1Ac antibody | OX-27
Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 (MCA156R) used for the assessment of chimerism in a rat transplant model by flow cytometry.
Image caption:
Peripheral blood multilineage chimerism in Group 5. Multilineage peripheral blood persisted for 12-13 weeks following vascularized hind-limb transplant, carried out by hind-limb transplant (HLT), at a low level in Group 5. At 13 weeks following vascularized hind-limb transplant, peripheral blood chimerism could hardly be detected by flow cytometry in 5 of 6 tolerant recipients in Group 5.
From: Quan Liu, Yong Wang, Atsunori Nakao, Wensheng Zhang, Vijay Gorantla, and Xin Xiao Zheng, “Heart Allograft Tolerance Induced and Maintained by Vascularized Hind-Limb Transplant in Rats,”
Clinical and Developmental Immunology, vol. 2013, Article ID 483856, 6 pages, 2013.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC class I antibody, clone OX-27 (MCA156R) used for the determination of levels of chimerism in a rat transplant model by flow cytometry and immunofluorescence
Image caption:
Flow cytometry analysis for myeloid chimerism and immunocytofluorescence staining for the presence of donor-origin cells in recipients' innate femurs. (a)The proportion of donor-origin cells observed from 2 representative recipients at POD 30 were at 5.7% and 5.5%, respectively, with the background level assessed at 0.1%. (b, c) The presence of RT1n cells in the contralateral femurs of recipients in group 5 and their absence in other groups was confirmed by immunocytofluorescence (×20).
From: Wang Y, Zheng Z, Wang Y, Liu J, Li N, et al. (2012)
Role of Donor-Specific Regulatory T Cells in Long-Term Acceptance of Rat Hind Limb Allograft.
PLoS ONE 7(8): e43825.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC class I RT1Ac antibody, clone OX-27 (MCA156R) used for the evaluation of peripheral chimerism in a rat transplant model by flow cytometry.
Image caption:
Dynamic changes in peripheral and myeloid chimerism. (a) Time kinetics of chimerism in groups 5 and 6. At POD 7, they showed almost the same chimerism level (P>0.05). At POD 21, the level of chimerism in group 6 was mildly decreased, but it increased sharply in group 5 (P<0.05). At POD 42, chimerism in group 5 returned to a total level 8.7%±0.6% but it decreased to background level in group 6 (P<0.05). In addition, donor-origin CD45RA+ B cells in group 5 prevailed beginning at POD 21 and maintained thereafter. (b) Myeloid chimerism was detectable at 6.3±0.5% at POD 100 in group 5 and it never appeared in group 6 when allografts were rejected (n = 6, P<0.05).
From: Wang Y, Zheng Z, Wang Y, Liu J, Li N, et al. (2012)
Role of Donor-Specific Regulatory T Cells in Long-Term Acceptance of Rat Hind Limb Allograft.
PLoS ONE 7(8): e43825.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat MHC Class I RT1Ac:FITC
- Product Type
- Monoclonal Antibody
- Clone
- OX-27
- Isotype
- IgG2a
- Specificity
- MHC Class I RT1Ac
Antibody Quality and Performance Guarantee
| Mouse anti Rat MHC Class I RT1Ac antibody, clone OX-27 recognizes a polymorphic determinant (c haplotype) of rat Class I MHC Antigen (RT-1A). |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1.0% Bovine Serum Albumin - Immunogen
- PHA activated rat lymphocytes.
- Approx. Protein Concentrations
- Isotype concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA1210F | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG2a Negative Control:FITC | ||||||
References for MHC Class I RT1Ac antibody
-
Hikita, N. et al. (1997) Use of topical FK506 in a corneal graft rejection model in Lewis rats.
Invest Ophthalmol Vis Sci. 38 (5): 901-9. -
Sharland, A. et al. (1999) Evidence that apoptosis of activated T cells occurs in spontaneous tolerance of liver allografts and is blocked by manipulations which break tolerance.
Transplantation. 68:1736-45. -
Huang, W.C. et al. (2010) Vascularized bone grafts within composite tissue allotransplants can autocreate tolerance through mixed chimerism with partial myeloablative conditioning: an experimental study in rats.
Plast Reconstr Surg. 125 (4): 1095-103. -
Wang Y et al. (2012) Role of donor-specific regulatory T cells in long-term acceptance of rat hind limb allograft.
PLoS One. 7 (8): e43825. -
Liu, Q. et al. (2013) Heart allograft tolerance induced and maintained by vascularized hind-limb transplant in rats.
Clin Dev Immunol. 2013: 483856. -
Zhu, H. et al. (2015) Rat model of heterotopic toe allotransplantation.
J Surg Res. 199 (2): 707-17. -
Gu, C. et al. (2016) Triptolide Reduces the Required Dose of Tacrolimus by Attenuating Inflammation, Enhancing Immunosuppression, and Increasing Donor Chimerism in a Heterotopic Hindlimb Transplantation Model.
Plast Reconstr Surg. 138 (6): 1243-1253. -
von Websky, M.W. et al. (2016) Recombinant HLA-G as Tolerogenic Immunomodulant in Experimental Small Bowel Transplantation.
PLoS One. 11 (7): e0158907.
View The Latest Product References
-
Schweizer, R. et al. (2020) Adipose-derived stromal cell therapy combined with a short course nonmyeloablative conditioning promotes long-term graft tolerance in vascularized composite allotransplantation.
Am J Transplant. 20 (5): 1272-84.
- RRID
- AB_323675
MCA156F
154720
155953
1606
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with MHC CLASS I specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Rat ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up