Mouse anti Rat Kappa/Lambda Light Chain: antibody cocktail is a mixture of clones MARK-1 and MARL-15 recognizing rat kappa and lambda light chains respectively. The cocktail doe not recognize immunogloulin heavy chsims.
- Target Species
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- MARK-1: Rat kappa light chains (IR202 and IR968) MARL-15: Rat lambda light chains (RH58, IR31)
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 (MARK-1) or PAI-O (MARL-15) mouse myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Kappa/Lambda Light Chain antibody
||0.5ug/ml as detecting antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- HRP-conjugated MARK-1/MARL-15 clone cocktail may be used as a detection reagent for rat immunoglobulins in ELISA assays and can be used in combination with coating antibodies:
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
Product Specific References
References for Kappa/Lambda Light Chain antibody
Daley, S.R. et al. (2007) A key role for TGF-beta signaling to T cells in the long-term acceptance of allografts.
J Immunol. 179 (6): 3648-54.
Daley, S.R .et al. (2008) Fc-disabled anti-mouse CD40L antibodies retain efficacy in promoting transplantation tolerance.
Am J Transplant. 8 (11): 2265-71.
Sun, Q. et al. (2012) Upregulated protein arginine methyltransferase 1 by IL-4 increases eotaxin-1 expression in airway epithelial cells and participates in antigen-induced pulmonary inflammation in rats.
J Immunol. 188 (7): 3506-12.
Cousins, L. et al. (2006) Eosinophilic bowel disease controlled by the BB rat-derived lymphopenia/Gimap5 gene.
Sandin, L.C. et al. (2014) Locally delivered CD40 agonist antibody accumulates in secondary lymphoid organs and eradicates experimental disseminated bladder cancer.
Cancer Immunol Res. 2 (1): 80-90.
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