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Kappa/Lambda Light Chain antibody | MARK-1 / MARL-15

Mouse anti Rat Kappa/Lambda Light Chain:HRP

Product Type
Monoclonal Antibody
MARK-1 / MARL-15
Kappa/Lambda Light Chain

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
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Mouse anti Rat Kappa/Lambda Light Chain: antibody cocktail is a mixture of clones MARK-1 and MARL-15 recognizing rat kappa and lambda light chains respectively. The cocktail doe not recognize immunogloulin heavy chsims.

Target Species
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
50% glycerol
MARK-1: Rat kappa light chains (IR202 and IR968) MARL-15: Rat lambda light chains (RH58, IR31)
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 (MARK-1) or PAI-O (MARL-15) mouse myeloma cell line.
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml as detecting antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
HRP-conjugated MARK-1/MARL-15 clone cocktail may be used as a detection reagent for rat immunoglobulins in ELISA assays and can be used in combination with coating antibodies:

rat IgG1MCA194
rat IgG2aMCA278
rat IgG2bMCA1294
rat IgG2cMCA1295
rat IgMMCA189
rat IgEMCA193
rat IgAMCA191

Description Product Code Applications Pack Size List Price Your Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus
TMB Signal+ BUF054A E 100 ml
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Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core
TMB Core+ BUF062A E 100 ml
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Description TMB Core+

References for Kappa/Lambda Light Chain antibody

  1. Cousins, L. et al. (2006) Eosinophilic bowel disease controlled by the BB rat-derived lymphopenia/Gimap5 gene.
    Gastroenterology. 131:1475-85.
  2. Daley, S.R. et al. (2007) A key role for TGF-beta signaling to T cells in the long-term acceptance of allografts.
    J Immunol. 179 (6): 3648-54.
  3. Daley, S.R .et al. (2008) Fc-disabled anti-mouse CD40L antibodies retain efficacy in promoting transplantation tolerance.
    Am J Transplant. 8 (11): 2265-71.
  4. Sun, Q. et al. (2012) Upregulated protein arginine methyltransferase 1 by IL-4 increases eotaxin-1 expression in airway epithelial cells and participates in antigen-induced pulmonary inflammation in rats.
    J Immunol. 188 (7): 3506-12.
  5. Sandin, L.C. et al. (2014) Locally delivered CD40 agonist antibody accumulates in secondary lymphoid organs and eradicates experimental disseminated bladder cancer.
    Cancer Immunol Res. 2 (1): 80-90.

Entrez Gene
GO Terms
GO:0003823 antigen binding


151253 161206 163649

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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