Kappa/Lambda Light Chain antibody | MARK-1/MARL-15

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Mouse anti Rat Kappa/Lambda Light Chain:HRP

Product Type
Monoclonal Antibody
Clone
MARK-1/MARL-15
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1296P E 0.5 mg
Mouse anti Rat Kappa/Lambda Light Chain: antibody cocktail is a mixture of clones MARK-1 and MARL-15 recognizing rat kappa and lambda light chains respectively. The cocktail doe not recognize immunogloulin heavy chsims.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
50%Glycerol
0.01%Thiomersal
Immunogen
MARK-1: Rat kappa light chains (IR202 and IR968) MARL-15: Rat lambda light chains (RH58, IR31)
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 (MARK-1) or PAI-O (MARL-15) mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01835 Related reagents
P01836 Related reagents
P20766 Related reagents
P20767 Related reagents
Entrez Gene
LOC500180 Related reagents
RGD1564318 Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of Kappa/Lambda Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.5ug/ml as detecting antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
HRP-conjugated MARK-1/MARL-15 clone cocktail may be used as a detection reagent for rat immunoglobulins in ELISA assays and can be used in combination with coating antibodies:

rat IgG1MCA194
rat IgG2aMCA278
rat IgG2bMCA1294
rat IgG2cMCA1295
rat IgMMCA189
rat IgEMCA193
rat IgAMCA191

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Kappa/Lambda Light Chain antibody

  1. Daley, S.R. et al. (2007) A key role for TGF-beta signaling to T cells in the long-term acceptance of allografts.
    J Immunol. 179 (6): 3648-54.
  2. Daley, S.R .et al. (2008) Fc-disabled anti-mouse CD40L antibodies retain efficacy in promoting transplantation tolerance.
    Am J Transplant. 8 (11): 2265-71.
  3. Sun, Q. et al. (2012) Upregulated protein arginine methyltransferase 1 by IL-4 increases eotaxin-1 expression in airway epithelial cells and participates in antigen-induced pulmonary inflammation in rats.
    J Immunol. 188 (7): 3506-12.
  4. Cousins, L. et al. (2006) Eosinophilic bowel disease controlled by the BB rat-derived lymphopenia/Gimap5 gene.
    Gastroenterology. 131:1475-85.
  5. Sandin, L.C. et al. (2014) Locally delivered CD40 agonist antibody accumulates in secondary lymphoid organs and eradicates experimental disseminated bladder cancer.
    Cancer Immunol Res. 2 (1): 80-90.