Browsing Modes for Business Users

IgG2a antibody | MARG2a-1

ISO 9001 Quality Assurance Manufacturing | Print
Anti Rat IgG2a Heavy Chain Antibody, clone MARG2a-1 thumbnail image 1
Anti Rat IgG2a Heavy Chain Antibody, clone MARG2a-1 gallery image 1
Published customer image:
Biotinylated Mouse anti Rat IgG2a antibody, clone Marg2a-1 (MCA278B) used to label bound anti CD169 antibody by flow cytometry.
Image caption:
Sialoadhesin is expressed on BM-Mø and is upregulated upon C. jejuni LOS stimulation. Cells were stimulated overnight with GB11 wt or Cst-II mutant LOS and sialoadhesin expression was examined by flow cytometry. Sialoadhesin was expressed on unstimulated BM-Mø (A; dotted line) and was upregulated after stimulation with GB11 LOS (B, and solid line in A). Filled histograms represent background fluorescence when incubated with the secondary antibody alone. BM-DC only express low levels of sialoadhesin and do not upregulate sialoadhesin in response to GB11 LOS (C).

From: Huizinga R, Easton AS, Donachie AM, Guthrie J, van Rijs W, Heikema A, Boon L, Samsom JN, Jacobs BC, Willison HJ, Goodyear CS.
Sialylation of Campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice.
PLoS One. 2012;7(3):e34416.
doi: 10.1371/journal.pone.0034416.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Rat IgG2a Heavy Chain:Biotin

Product Type
Monoclonal Antibody
Clone
MARG2a-1
Isotype
IgG1
Specificity
IgG2a
Region
HEAVY CHAIN

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA278B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
List Price Your Price
loader

Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 recognizes the rat gamma 2a immunoglobulin heavy chain, and does not cross-react with other immunoglobulin classes or subclasses.

Avidity of Mouse anti Rat IgG2a heavy chain antibody, clone MARG2a-1 for rat IgG2a = 2.1 x 109 M-1.

Target Species
Rat
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide
50%Glycerol
Immunogen
Purified rat IgG2a.
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the PAI-0 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 500ng/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for IgG2a antibody

  1. Bazin, H. et al. (1973) Brief communication: Transplantable immunoglobulin-secreting tumors in rats. V. Monoclonal immunoglobulins secreted by 250 ileocecal immunocytomas in LOU-Wsl rats.
    J Natl Cancer Inst. 51 (4): 1359-61.
  2. Bazin, H. et al. (1974) Three classes and four (sub)classes of rat immunoglobulins: IgM, IgA, IgE and IgG1, IgG2a, IgG2b, IgG2c.
    Eur J Immunol. 4 (1): 44-8.
  3. Sandin, L.C. et al. (2014) Locally delivered CD40 agonist antibody accumulates in secondary lymphoid organs and eradicates experimental disseminated bladder cancer.
    Cancer Immunol Res. 2 (1): 80-90.
  4. Bézie, S. et al. (2015) Fibrinogen-like protein 2/fibroleukin induces long-term allograft survival in a rat model through regulatory B cells.
    PLoS One. 10 (3): e0119686.
  5. Bézie S et al. (2015) Compensatory Regulatory Networks between CD8 T, B, and Myeloid Cells in Organ Transplantation Tolerance.
    J Immunol. 195 (12): 5805-15.
  6. Ueta, H. et al. (2018) Single blood transfusion induces the production of donor-specific alloantibodies and regulatory T cells mainly in the spleen.
    Int Immunol. 30 (2): 53-67.
  7. Huizinga, R. et al. (2012) Sialylation of Campylobacter jejuni lipo-oligosaccharides: impact on phagocytosis and cytokine production in mice.
    PLoS One. 7 (3): e34416.

Further Reading

  1. Köhler, G. & Milstein, C. (1975) Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 256 (5517): 495-7.
  2. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat.
    J. Immunol. 121: 2077-2082.
  3. Shulman, M. et al. (1978) A better cell line for making hybridomas secreting specific antibodies.
    Nature. 276 (5685): 269-70.

RRID
AB_321826
UniProt
P20760
Entrez Gene
LOC679045
GO Terms
GO:0003823 antigen binding

MCA278B

160721 160898

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with IgG specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products
Please note