CD90 antibody | OX-7
Mouse anti Rat CD90:Alexa Fluor® 488
Mouse anti Rat CD90:Alexa Fluor® 647
Mouse anti Rat CD90:FITC
Mouse anti Rat CD90:Low Endotoxin
Mouse anti Rat CD90
Mouse anti Rat CD90:RPE
- Product Type
- Monoclonal Antibody
- Clone
- OX-7
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| MCA47A488 | F | 100 Tests/1ml |
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| MCA47A488T | F | 25 Tests/0.25ml |
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| MCA47A647 | F | 100 Tests/1ml |
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| MCA47A647T | F | 25 Tests/0.25ml |
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| MCA47FA | F | 50 µg |
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| MCA47FT | F | 0.1 mg |
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| MCA47FTT | F | 25 µg |
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| MCA47EL | C F IF IP WB | 0.5 mg |
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| MCA47G | C F IF IP WB | 1 mg |
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| MCA47GA | C F IF IP | 0.1 mg |
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| MCA47R | C F IF IP WB | 0.25 mg |
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| MCA47RT | C F IF IP WB | 25 µg |
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| MCA47PE | F | 100 Tests |
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Mouse anti Rat CD90 antibody, clone OX-7 recognizes rat and CD90, also known as Thy1.1, a GPI-anchored membrane protein containing a single V type Ig-like domain CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, immature B cells and connective tissues, CD90 is also expressed in T cells in mice.
Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab′ of MRC OX-7 for rat Thy1 is 3 x 109m-1 and for mouse Thy1.1 is 3 x 108m-1(1).
Mouse anti rat CD90, clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (Jeng et al. 1998). Clone OX-7 has also been reported to induce glomerular nephritis in Wistar rats (Tamura et al. 1996).
This product is routinely tested in flow cytometry on rat thymocytes.
Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab′ of MRC OX-7 for rat Thy1 is 3 x 109m-1 and for mouse Thy1.1 is 3 x 108m-1(1).
Mouse anti rat CD90, clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (Jeng et al. 1998). Clone OX-7 has also been reported to induce glomerular nephritis in Wistar rats (Tamura et al. 1996).
This product is routinely tested in flow cytometry on rat thymocytes.
Product Details
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Mouse Guinea Pig - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Rat Thy1 antigen.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 6 months from date of despatch
More Information
- UniProt
- P01830
- Entrez Gene
- Thy1
- GO Terms
- GO:0001525 angiogenesis
- GO:0007010 cytoskeleton organization
- GO:0005829 cytosol
- GO:0005100 Rho GTPase activator activity
- GO:0006469 negative regulation of protein kinase activity
- GO:0016337 cell-cell adhesion
- GO:0019901 protein kinase binding
- GO:0030336 negative regulation of cell migration
- GO:0030426 growth cone
- GO:0034235 GPI anchor binding
- GO:0043066 negative regulation of apoptosis
- GO:0043547 positive regulation of GTPase activity
- GO:0045576 mast cell activation
- GO:0046549 retinal cone cell development
- GO:0048041 focal adhesion assembly
- GO:0048147 negative regulation of fibroblast proliferation
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050771 negative regulation of axonogenesis
- GO:0050852 T cell receptor signaling pathway
- GO:0050860 negative regulation of T cell receptor signaling pathway
- GO:0050870 positive regulation of T cell activation
- GO:0051281 positive regulation of release of sequestered calcium ion into cytosol
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD90 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | 1/40 | 1/80 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | 1/50 | 1/100 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | Neat | 1/10 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA1209A488 | F | 100 Tests/1ml |
|
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| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
|
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| Mouse IgG1 Negative Control:FITC | MCA1209F | F | 0.1 mg |
|
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| Mouse IgG1 Negative Control:Low Endotoxin | MCA1209EL | F | 0.5 mg |
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| Mouse IgG1 Negative Control | MCA1209 | F | 0.1 mg |
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| Mouse IgG1 Negative Control:RPE | MCA1209PE | F | 100 Tests |
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Application Based External Images
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blotting
Product Specific References
References for CD90 antibody
-
Mason, D.W. & Williams, A.F. (1980) The kinetics of antibody binding to membrane antigens in solution and at the cell surface.
Biochem J. 187 (1): 1-20. -
Campbell, D.G. et al. (1981) Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region.
Biochem J. 195 (1): 15-30. -
Bukovský, A. et al. (1983) The localization of Thy-1.1, MRC OX 2 and Ia antigens in the rat ovary and fallopian tube.
Immunology. 48 (3): 587-96. -
Lee, W.S. et al. (1998) Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines.
Circ Res. 82 (8): 845-51. -
Jeng, C.J. et al. (1998) Thy-1 is a component common to multiple populations of synaptic vesicles.
J Cell Biol. 140 (3): 685-98. -
Banerjee, S.A. et al. (1997) An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
J Neurosci. 17 (8): 2756-65. -
Kawachi, H. et al. (1992) Epitope-specific induction of mesangial lesions with proteinuria by a MoAb against mesangial cell surface antigen.
Clin Exp Immunol. 88 (3): 399-404. -
Tamura, M. et al. (1996) Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis.
Biochem Biophys Res Commun. 222 (3): 683-7. -
Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
Stem Cells Dev. 18 (10): 1389-98. -
Biermann, J. et al. (2011) Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells.
Mol Vis. 17: 395-403. -
Keller, R.K. et al. (2004) Formation of 7-dehydrocholesterol-containing membrane rafts in vitro and in vivo, with relevance to the Smith-Lemli-Opitz syndrome.
J Lipid Res. 45: 347-55. -
Ohashi, N. et al. (2010) Glomerular angiotensinogen is induced in mesangial cells in diabetic rats via reactive oxygen species--ERK/JNK pathways.
Hypertens Res. 33:1174-81. -
Maia L et al. (2016) Conditioned medium: A new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
Cell Biol Int. Nov 26. [Epub ahead of print] -
Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
J Immunol Methods. 336: 71-7. -
Freisinger, W. et al. (2013) Sensory renal innervation: a kidney-specific firing activity due to a unique expression pattern of voltage-gated sodium channels?
Am J Physiol Renal Physiol. 304: F491-7. -
Shimizu T et al. (2016) Bioactivity of sol-gel-derived TiO2 coating on polyetheretherketone: In vitro and in vivo studies.
Acta Biomater. 35: 305-17. -
Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
Theriogenology. 100: 8-15. -
Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92. -
Huang, X. et al. (2019) MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.
Contrast Media Mol Imaging. 2019: 5184105. -
Zhao, Y. et al. (2017) A new electrospun graphene-silk fibroin composite scaffolds for guiding Schwann cells.
J Biomater Sci Polym Ed. 28 (18): 2171-85.
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