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CD90 antibody | OX-7

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Anti Rat CD90 Antibody, clone OX-7 thumbnail image 1 Anti Rat CD90 Antibody, clone OX-7 thumbnail image 2 Anti Rat CD90 Antibody, clone OX-7 thumbnail image 3 Anti Rat CD90 Antibody, clone OX-7 thumbnail image 4 Anti Rat CD90 Antibody, clone OX-7 thumbnail image 5 Anti Rat CD90 Antibody, clone OX-7 thumbnail image 6
Anti Rat CD90 Antibody, clone OX-7 gallery image 1
Figure A. FITC conjugated Mouse anti Rat CD3 (MCA772F) and PE conjugated Mouse IgG1 isotype control (MCA1209PE). Figure B. FITC conjugated Mouse anti Rat CD3 (MCA772F) and PE conjugated Mouse anti Rat CD90 (MCA47PE). All experiments performed on red cell lysed rat blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer.
Anti Rat CD90 Antibody, clone OX-7 gallery image 2
Figure A. A647 conjugated mouse anti rat CD4 (MCA55A647) and PE conjugated mouse IgG1 isotype control (MCA1209PE). Figure B. A647 conjugated mouse anti rat CD4 (MCA55A647) and PE conjugated mouse anti rat CD90 (MCA47PE). All experiments performed on rat thymocytes.
Anti Rat CD90 Antibody, clone OX-7 gallery image 3
Figure A. PE conjugated mouse anti rat CD90 (MCA47PE) and A647 conjugated mouse IgG1 isotype control (MCA1209A647). Figure B. PE conjugated mouse anti rat CD90 (MCA47PE) and A647 conjugated mouse anti rat CD4 (MCA55A647). All experiments performed on rat thymocytes.
Anti Rat CD90 Antibody, clone OX-7 gallery image 4
Published customer image:
Mouse anti Rat CD90 antibody, clone OX-7 (MCA47R) used to label THY-1 expressing cells in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
Paracortex of the rat popliteal lymph node in initial massive S. epidermidis infection (days 1–7) with early node’s evaluation (day 8) stained for the presence of large stem cells, thymocytes and immature B cells (CD90+, red). Magnification 1000×.

From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat CD90 Antibody, clone OX-7 gallery image 5
Published customer image:
Mouse anti Rat CD90 antibody, clone OX-7 (MCA47R) used to label THY-1 expressing cells in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
The medulla of the rat popliteal lymph node in initial massive S. epidermidis infection (days 1–7) with early node’s evaluation (day 8). The node stained for: (A) Dendritic cells (OX62+, red). (B) Macrophages and monocytes (CD68+, red). (C) Stem cells, thymocytes and immature B cells (CD90+, red). (D) T helper lymphocytes and monocytes (CD4+, red). (E) Activated antigen-presenting cells (MHC class II+, red). Magnification 1000×.

From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat CD90 Antibody, clone OX-7 gallery image 6
Published customer image:
Mouse anti Rat CD90 antibody, clone OX-7 (MCA47R) used to sort THY-1 expressing cells from rat liver by flow cytometry.
Image caption:
Transplantation of Thy1-LSPCs and Thy1-MCs to Ret/PH-treated rat livers.
(A) Schematic showing the cell transplantation method.

From: Ichinohe, N. et al.
CINC-2 and miR-199a-5p in exosomes secreted by transplanted Thy1+ cells activate hepatocytic progenitor cell growth in rat liver regeneration.
Res Sq. 20 Jan [Preprint].
doi: 10.21203/rs.3.rs-2087658/v1 
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Rat CD90:RPE

Product Type
Monoclonal Antibody
Clone
OX-7
Isotype
IgG1
Specificity
CD90

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Product Code Applications Pack Size List Price Your Price Qty
MCA47PE
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F 100 Tests loader
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Mouse anti Rat CD90 antibody, clone OX-7 recognizes rat and CD90, also known as Thy1.1, a GPI-anchored membrane protein containing a single V type Ig-like domain CD90 is expressed on a variety of cell types including thymocytes, neuronal cells, stem cells, immature B cells and connective tissues, CD90 is also expressed in T cells in mice.

Since Thy1.1 is a monomorphic determinant in rat but polymorphic in mice, clone MRC OX-7 reacts with Thy1.1 mice e.g. AKR and FVB, but not Thy1.2 mice such as CBA and BALB/c. The affinity of the Fab′ of MRC OX-7 for rat Thy1 is 3 x 109m-1 and for mouse Thy1.1 is 3 x 108m-1(1).

Mouse anti rat CD90, clone MRC OX-7 has been demonstrated to promote neurite outgrowths on peripherin-stained sympathetic rat neurons, using fluorescence microscopy (Jeng et al. 1998). Clone OX-7 has also been reported to induce glomerular nephritis in Wistar rats (Tamura et al. 1996).

This product is routinely tested in flow cytometry on rat thymocytes.

Target Species
Rat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Mouse
Guinea Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Rat Thy1 antigen.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Source Reference

  1. Mason, D.W. & Williams, A.F. (1980) The kinetics of antibody binding to membrane antigens in solution and at the cell surface.
    Biochem J. 187 (1): 1-20.

References for CD90 antibody

  1. Campbell, D.G. et al. (1981) Rat brain Thy-1 glycoprotein. The amino acid sequence, disulphide bonds and an unusual hydrophobic region.
    Biochem J. 195 (1): 15-30.
  2. Bukovský, A. et al. (1983) The localization of Thy-1.1, MRC OX 2 and Ia antigens in the rat ovary and fallopian tube.
    Immunology. 48 (3): 587-96.
  3. Kawachi, H. et al. (1992) Epitope-specific induction of mesangial lesions with proteinuria by a MoAb against mesangial cell surface antigen.
    Clin Exp Immunol. 88 (3): 399-404.
  4. Tamura, M. et al. (1996) Enhanced glomerular profilin gene and protein expression in experimental mesangial proliferative glomerulonephritis.
    Biochem Biophys Res Commun. 222 (3): 683-7.
  5. Banerjee, S.A. et al. (1997) An antibody to the tetraspan membrane protein CD9 promotes neurite formation in a partially alpha3beta1 integrin-dependent manner.
    J Neurosci. 17 (8): 2756-65.
  6. Lee, W.S. et al. (1998) Thy-1, a novel marker for angiogenesis upregulated by inflammatory cytokines.
    Circ Res. 82 (8): 845-51.
  7. Keller, R.K. et al. (2004) Formation of 7-dehydrocholesterol-containing membrane rafts in vitro and in vivo, with relevance to the Smith-Lemli-Opitz syndrome.
    J Lipid Res. 45: 347-55.
  8. Rutigliano, J.A. et al. (2008) Screening monoclonal antibodies for cross-reactivity in the ferret model of influenza infection.
    J Immunol Methods. 336: 71-7.
    9. View The Latest Product References
  9. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
    Stem Cells Dev. 18 (10): 1389-98.
  10. Ohashi, N. et al. (2010) Glomerular angiotensinogen is induced in mesangial cells in diabetic rats via reactive oxygen species--ERK/JNK pathways.
    Hypertens Res. 33:1174-81.
  11. Biermann, J. et al. (2011) Histone deacetylase inhibitors sodium butyrate and valproic acid delay spontaneous cell death in purified rat retinal ganglion cells.
    Mol Vis. 17: 395-403.
  12. Freisinger, W. et al. (2013) Sensory renal innervation: a kidney-specific firing activity due to a unique expression pattern of voltage-gated sodium channels?
    Am J Physiol Renal Physiol. 304: F491-7.
  13. Shimizu T et al. (2016) Bioactivity of sol-gel-derived TiO2 coating on polyetheretherketone: In vitro and in vivo studies.
    Acta Biomater. 35: 305-17.
  14. Maia, L. et al. (2017) Conditioned medium: a new alternative for cryopreservation of equine umbilical cord mesenchymal stem cells.
    Cell Biol Int. 41 (3): 239-48.
  15. Maia, L. et al. (2017) A proteomic study of mesenchymal stem cells from equine umbilical cord.
    Theriogenology. 100: 8-15.
  16. Zhao, Y. et al. (2017) A new electrospun graphene-silk fibroin composite scaffolds for guiding Schwann cells.
    J Biomater Sci Polym Ed. 28 (18): 2171-85.
  17. Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
    Exp Clin Transplant. 17 (1): 84-92.
  18. Huang, X. et al. (2019) MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.
    Contrast Media Mol Imaging. 2019: 5184105.
  19. Kuriyama, T. et al. (2020) A novel rat model of inflammatory bowel disease developed using a device created with a 3D printer.
    Regen Ther. 14: 1-10.
  20. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
    Biomedicines. 10 (5): 1021.
  21. Huang, S. et al. (2022) Hydrogen sulfide supplement preserves mitochondrial function of retinal ganglion cell in a rat glaucoma model.
    Cell Tissue Res. 389 (2): 171-85.
  22. Cacciamali, A. et al. (2022) Engineered nanoparticles toxicity on adipose tissue derived mesenchymal stem cells: A preliminary investigation
    Res Vet Sci. 152: 134-49.
  23. Eweida, A. et al. (2022) Systemically injected bone marrow mononuclear cells specifically home to axially vascularized tissue engineering constructs.
    PLoS One. 17 (8): e0272697.
  24. Ichinohe, N. et al. (2023) CINC-2 and miR-199a-5p in EVs secreted by transplanted Thy1(+) cells activate hepatocytic progenitor cell growth in rat liver regeneration.
    Stem Cell Res Ther. 14 (1): 134.
  25. Numata-Uematasu, Y. et al. (2023) In vitro myelination using explant culture of dorsal root ganglia: An efficient tool for analyzing peripheral nerve differentiation and disease modeling.
    PLoS One. 18 (5): e0285897.

Flow Cytometry

Immunofluorescence

Western Blotting

Synonyms
THY1
RRID
AB_321893
UniProt
P01830
Entrez Gene
Thy1
GO Terms
GO:0001525 angiogenesis
GO:0007010 cytoskeleton organization
GO:0005829 cytosol
GO:0005100 Rho GTPase activator activity
GO:0006469 negative regulation of protein kinase activity
GO:0016337 cell-cell adhesion
GO:0019901 protein kinase binding
GO:0030336 negative regulation of cell migration
GO:0030426 growth cone
GO:0034235 GPI anchor binding
GO:0043066 negative regulation of apoptosis
GO:0043547 positive regulation of GTPase activity
GO:0045576 mast cell activation
GO:0046549 retinal cone cell development
GO:0048041 focal adhesion assembly
GO:0048147 negative regulation of fibroblast proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050771 negative regulation of axonogenesis
GO:0050852 T cell receptor signaling pathway
GO:0050860 negative regulation of T cell receptor signaling pathway
GO:0050870 positive regulation of T cell activation
GO:0051281 positive regulation of release of sequestered calcium ion into cytosol

MCA47PE

158952 167690 INN1708

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