CD86 antibody | 24F

Mouse anti Rat CD86:RPE

Product Type
Monoclonal Antibody
Clone
24F
Isotype
IgG1
Specificity
CD86

Product Code Applications Pack Size List Price Your Price Qty
MCA2874PE
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Mouse anti Rat CD86 antibody, clone 24F recognizes rat CD86, otherwise known as B7-2, a type I transmembrane protein and member of the Ig superfamily, which acts as a ligand for both CD28 and CD152 (CTLA-4), and is primarily expressed on antigen presenting cells (APCs) including dendritic cells, and also on germinal centre B cells and macrophages.

Like CD80, CD86 is an accessory molecule which functions in the CD28-CD80/CD86 co-stimulatory pathway, vital for T cell activation, crosstalk between T and B cells, and Th2-mediated Ig production.

Mouse anti Rat CD86 antibody, clone 24F has been shown to block the co-stimulatory activity of rat CD86 (Maeda et al. 1997).

Target Species
Rat
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
HTLV-1 transformed Lewis-S1 cells.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the P3U1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader
List Price Your Price
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Description Mouse IgG1 Negative Control:RPE

References for CD86 antibody

  1. Maeda, K. et al. (1997) Characterization of rat CD80 and CD86 by molecular cloning and mAb.
    Int Immunol. 9 (7): 993-1000.
  2. Damoiseaux, J.G. et al. (1998) Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells.
    J Leukoc Biol. 64 (6): 803-9.
  3. Hanabuchi, S. et al. (2000) Development of human T-cell leukemia virus type 1-transformed tumors in rats following suppression of T-cell immunity by CD80 and CD86 blockade.
    J Virol. 74: 428-35.
  4. Kano, M. et al. (1998) A crucial role of host CD80 and CD86 in rat cardiac xenograft rejection in mice.
    Transplantation. 65: 837-43.
  5. Tamatani, T. et al. (2000) AILIM/ICOS: a novel lymphocyte adhesion molecule.
    Int Immunol. 12: 51-5.
  6. Dilek, N. et al. (2012) Control of transplant tolerance and intragraft regulatory T cell localization by myeloid-derived suppressor cells and CCL5.
    J Immunol. 188: 4209-16.
  7. Ghiringhelli, F. et al. (2005) Tumor cells convert immature myeloid dendritic cells into TGF-beta-secreting cells inducing CD4+CD25+ regulatory T cell proliferation.
    J Exp Med. 202: 919-29.
  8. Sacedón, R. et al. (1999) Glucocorticoid-mediated regulation of thymic dendritic cell function.
    Int Immunol. 11: 1217-24.
  9. View The Latest Product References
  10. Kawai, T. et al. (2000) T(h)1 transmigration anergy: a new concept of endothelial cell-T cell regulatory interaction.
    Int Immunol. 12: 937-48.
  11. Macphee, I.A. et al. (2002) The Th2-response in mercuric chloride-induced autoimmunity requires continuing costimulation via CD28.
    Clin Exp Immunol. 129: 405-10.
  12. MacPhee, I.A. et al. (2006) Blockade of OX40-ligand after initial triggering of the T helper 2 response inhibits mercuric chloride-induced autoimmunity.
    Immunology. 117: 402-8.
  13. Yrlid, U. et al. (2006) A distinct subset of intestinal dendritic cells responds selectively to oral TLR7/8 stimulation.
    Eur J Immunol. 36: 2639-48.
  14. Matsumoto, S. et al. (2015) CD200+ and CD200- macrophages accumulated in ischemic lesions of rat brain: the two populations cannot be classified as either M1 or M2 macrophages.
    J Neuroimmunol. 282: 7-20.
  15. Patil, P.S. et al. (2016) Fluorinated methacrylamide chitosan hydrogels enhance collagen synthesis in wound healing through increased oxygen availability.
    Acta Biomater. 36: 164-74.
  16. Hellenbrand, D.J. et al. (2019) Sustained interleukin-10 delivery reduces inflammation and improves motor function after spinal cord injury.
    J Neuroinflammation. 16 (1): 93.

Synonyms
B7-2
RRID
AB_2075122
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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