CD8 Alpha antibody | OX-8
Mouse anti Rat CD8 alpha antibody, clone OX-8 used for the identification of cytotoxic T cells in tumors by immunofluorescence.
Image caption:
Immunohistochemical labeling of tumor thin sections with CD8 (red) antibodies and DAPI (blue) nuclear stain. A: Two typical sections from an untreated control tumor collected 14 days after injecting tumor cells into the liver; B. Two typical sections of a secondary tumor that was fixed 7 days after it was injected into a liver in which the primary tumor had been nanoelectroablated 3 weeks earlier. This secondary tumor exhibited growth inhibition and a photograph of it can be seen in Fig 5B; C. Percentage of cells expressing CD8 in histological sections of tumors from four different conditions. Each bar represents the mean of measurements taken from 3–7 sections of 2 separate tumors. The untreated tumors were fixed 14 days after injection; “ns+7d” represents tumors fixed 7days after treating with 400 pulses, 15 kV, 100ns; “T2+7d” represents secondary tumors fixed 7 days after injection. CD8 concentration is significantly different from control tumor levels with **p = 0.002; “T2+7d+CD9 AB” represents secondary tumors fixed 7 days after injection with CD8 antibodies injected IP 24 h prior to the tumor cell injection. The CD8 concentration is significantly different from control tumors with *p = 0.005.
From: Nuccitelli R, Berridge JC, Mallon Z, Kreis M, Athos B, Nuccitelli P (2015)
Nanoelectroablation of Murine Tumors Triggers a CD8-Dependent Inhibition of Secondary Tumor Growth.
PLoS ONE 10(7): e0134364.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD8α antibody, clone OX-8 (MCA48G) used for the detection of CD8 positive cells by flow cytometry.
Image caption
Qualitative and quantitative flow cytometric analysis of lymphocyte populations in draining lymph nodes. A: Representative FACS plot of CD4+ CD8+ staining used to count T-helper cells, cytotoxic T-cells and CD4+ CD8+ double positive T-lymphocytes. Events acquired: 2×105. B: FACS plot example for B-cell detection. C: Representative FACS plot for NK cell assessment. NK-T cell were confirmed by CD3 expression (not shown). Bar diagrams: Cumulative results for the quantification of major and minor lymphocyte populations in draining LN of cornea transplanted animals. An asterisk (*) indicates statistical significance at p≤0.05 determined by Mann–Whitney U-Test. Allo-Tx-d7 - animals allo-grafted and analyzed at day 07 post op, n=6; allo-Tx-rej – animals displaying allo rejection of grafted corneas analyzed after the onset of rejection, n=5; syn-Tx-d7 – syngeneically grafted animals analyzed at day 7 post-op, n=3; syn-Tx-LT – syn-grafted long-term survivors analyzed at the end of the observation period at day 42; n=3.
From: Maenz M, Morcos M, Ritter T.
A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
Mol Vis. 2011 Feb 8;17:420-9.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD8α antibody, clone OX-8 (MCA48G) used for the detection of CD8 positive cells by immunohistochemistry.
Image caption
CD4 and CD8 T cell infiltration. Photos show SN sections of an animal of the cell death group immunostained with antibody against CD4 (A and C) and CD8 (B and D). The small panels show insets in A (C) and in B (D) at higher magnification. Scale: 50 µm, applies to A–B, 10 µm applies to C–D. (E) Graph shows average (dash) and individual numbers of CD4+ cells found in one SN section per animal of each group plotted per time. Two-way ANOVA [F (8,42) = 4.1, p = 0.001 effect of group and time interaction] followed by Tukey HSD post-hoc analysis. ## or ç p<0.01 (##) compared to the other α-syn group at same time point; (††) different to the next time point of the same group. (F) Graph shows average (dash) and individual numbers of CD8+ cells found in one SN section per animal of each group plotted per time. Two-way ANOVA [F (8,41) = 4.3, p = 0.001 effect of group and time interaction] followed by Tukey HSD post-hoc analysis. (**) p<0.01 compared to all other groups at all time point.
From: Sanchez-Guajardo V, Febbraro F, Kirik D, Romero-Ramos M (2010)
Microglia Acquire Distinct Activation Profiles Depending on the Degree of α-Synuclein Neuropathology in a rAAV Based Model of Parkinson's Disease.
PLoS ONE 5(1): e8784.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD8α antibody, clone OX-8 (MCA48R) used for the detection of CD8 positive cells by immunohistochemistry.
Image caption
Analysis of graft-infiltrating T cells. Activated CD25+ T cells and CD4+ and CD8+ T cell subsets were stained at the time points of corneal allograft rejection and calculated within the graft. A, C, and E show representative histological staining for CD25, CD4, and CD8 in grafts of treated and control animals, respectively. CD25+ (B) and CD4+ (D) cells infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p<0.01, ** p<0.01). No statistical difference was observed for CD8+ T cells (NS=not significant; F).
From: Schwartzkopff J, Schlereth SL, Berger M, Bredow L, Birnbaum F, Böhringer D, Reinhard T.
NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 2010 Oct 2;16:1928-35.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD8α antibody, clone OX-8 (MCA48G) used for the detection of CD8 expressing cells in Bouin's fixed, paraffin embedded tissue sections by immunofluorescence.
Image caption:
CD3ε, CD8α, and PAX5 immunostainings. (A–D) CD3ε staining in the zone of the foramen of Monro (fM) of the injected ventricle comprising a part of the stria medullaris [sm, (A)]. Many positive cells occurred in the perivascular spaces of the local large venules 12 h after NA injection. A detailed view (B) of the vessels in the stria medullaris (sm) shows that many positive cells have smooth nuclei while polymorphonucleated cells (arrow) are negative. (C) Twenty-four hours after the injection, some positive cells seemed to cross the ependyma of the choroid plexus (cp). (D) Positive cells were also present in the meninges (men) next to the optic chiasm (oc), and few appeared to penetrate the brain parenchyma (arrow). (E–G) Double immunofluorescence of lymphocytes in a 12 h post-injection brain section. Most CD3ε-positive cells [(E), green] found in the perivascular spaces of the large vessels were also positive for CD8α [(F), red]. (H–J) PAX5 immunostaining showing positive cells in the wall of the large vessels around the foramen of Monro [fM; (H)], although they were not as abundant as CD3ε-positive cells. Few PAX5-positive nuclei were also found in the choroid plexus (cp) of the injected lateral ventricle (I) as well as in the meninges (men) close to the optic chiasm [oc; (J)]. cp, choroid plexus; fM, foramen of Monro; men, meninges; oc, optic chiasm; sm, stria medullaris.
From: Granados-Durán P, López-Ávalos MD, Grondona JM, Gómez-Roldán Mdel C, Cifuentes M, Pérez-Martín M, Alvarez M, Rodríguez de Fonseca F, Fernández-Llebrez P.
Neuroinflammation induced by intracerebroventricular injection of microbial neuraminidase.
Front Med (Lausanne). 2015 Mar 17;2:14.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Rat CD8 antibody, clone OX-8 (MCA48R) used to label cytotoxic T lymphocytes in meningeal sections of rat brain by immunofluorescence.
Image caption:
Injection of cytokine expressing vectors into the subarachnoid space of rats leads to meningeal inflammation.
Immunofluorescent staining showed the extensive infiltration of CD4+ and CD8+ T cells in the meninges within the sagittal sulcus and overlying the cortex of cytokine vector injected MOG animals.
From: James RE, Schalks R, Browne E, Eleftheriadou I, Munoz CP, Mazarakis ND, Reynolds R.
Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
Acta Neuropathol Commun. 2020 May 12;8(1):66.
doi: 10.1186/s40478-020-00938-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD8 antibody, clone OX-8 (MCA48R) used to label cytotoxic T lymphocytes in meningeal sections of rat brain by immunofluorescence.
Image caption:
Injection of cytokine expressing vectors into the subarachnoid space of rats leads to meningeal inflammation.
Immunofluorescent staining showed the extensive infiltration of CD4+ and CD8+ T cells in the meninges within the sagittal sulcus and overlying the cortex of cytokine vector injected MOG animals.
From: James RE, Schalks R, Browne E, Eleftheriadou I, Munoz CP, Mazarakis ND, Reynolds R.
Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
Acta Neuropathol Commun. 2020 May 12;8(1):66.
doi: 10.1186/s40478-020-00938-1.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD8 antibody, clone OX-8 (MCA48G) used to identify CD8+ in rat brain by immunohistochemistry on formalin fixed cryostat sections.
Image caption:
Immunohistochemical analysis of hESC-derived vmDA and vFB grafts. Activated microglia within the graft core and border (Ox42 [CD11b], Serotec, MCA275G, 1:1000) and CD8+ t-lymphocytes (Serotec, MC4A48G, 1:500) 20× magnification in each graft group.
From: Lane EL, Harrison DJ, Ramos-Varas E, Hills R, Turner S, Lelos MJ.
Spontaneous Graft-Induced Dyskinesias Are Independent of 5-HT Neurons and Levodopa Priming in a Model of Parkinson's Disease.
Mov Disord. 2021 Nov 12 [Epub ahead of print].
doi: 10.1002/mds.28856.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD8 antibody, clone OX-8 (MCA48R) used to label macrophages in rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Infiltrating cytotoxic T-cells after kidney transplantation in vehicle and Rapa treated rats.
CD8 positive T-cells in the cortex calculated as percentage of positive interstitial cells by immunohistochemistry. Representative photomicrographs are shown. n = 3. ***P<0.001.
From: Hoff U, Markmann D, Thurn-Valassina D, Nieminen-Kelhä M, Erlangga Z, Schmitz J, et al.. (2022)
The mTOR inhibitor Rapamycin protects from premature cellular senescence early after experimental kidney transplantation.
PLoS ONE 17(4): e0266319.
doi: 10.1371/journal.pone.0266319
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD8 antibody, clone OX-8 (MCA48R) used to label cytotoxic T lymphocytes in rat lymph nodes by immunohistochemistry on cryostat sections.
Image caption:
Paracortex of the rat popliteal lymph node stained for the presence of T cytotoxic lymphocytes (CD8+, red). (A) The node of the control animal receiving 0.9% NaCl (days 1–7, node evaluation on day 8). (B) The node after initial massive S. epidermidis infection (days 1–7) with early node evaluation (day 8). (C) The node after secondary S. epidermidis infection (day 28, node evaluation on day 29). Magnification 200×.
From: Cąkała-Jakimowicz, M.; Puzianowska-Kuznicka, M.
Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines 2022, 10, 1021.
doi: 10.3390/biomedicines10051021
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD3 antibody, clone 1F4 (MCA772) used to label lymphocytes in rat brain by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Infiltration of granule cell layer of cerebellar cortex of rats treated with methylmercury by cytotoxic T-lymphocytes. [[B] Immunostaining with a CD 8 antibody to detect cytotoxic T lymphocytes infiltrating the granule cell layer in rats treated with methylmercury (×200). Rat spleen tissue was used as a positive control. Arrowheads indicate CD 8-positive cells (cytotoxic T lymphocytes). T lymphocytes were detected on days 14, 21, and 28 after treatment with methylmercury.
From: Du, K. et al.
Pathogenesis of selective damage of granule cell layer in cerebellum of rats exposed to methylmercury.
J Toxicol Sci 48 (7): 429-39. (2023)
doi: 10.2131/jts.48.429
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Rat CD8 Alpha:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- OX-8
- Isotype
- IgG1
- Specificity
- CD8 Alpha
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD8α, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Mouse anti Rat CD8α, clone MRC OX-8 is suitable for use in in vitro blocking assays (Popov et al.2001). Mouse anti Rat CD8α, clone MRC OX-8 reacts with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (Whiteland et al. 1995). Mouse anti Rat CD8α, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes. |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- Rat thymocyte membrane glycoproteins.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
Source Reference
-
Brideau, R.J. et al. (1980) Two subsets of rat T lymphocytes defined with monoclonal antibodies.
Eur J Immunol. 10 (8): 609-15.
References for CD8 Alpha antibody
-
Thomas, M.L. & Green, J.R. (1983) Molecular nature of the W3/25 and MRC OX-8 marker antigens for rat T lymphocytes: comparisons with mouse and human antigens.
Eur J Immunol. 13 (10): 855-8. -
Bukovský A et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
Immunology. 52 (4): 631-40. -
Torres-Nagel, N. et al. (1992) Differential thymus dependence of rat CD8 isoform expression.
Eur J Immunol. 22 (11): 2841-8. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
J Histochem Cytochem. 43 (3): 313-20. -
Mitnacht, R. et al. (1998) Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats.
J Immunol. 160 (2): 700-7. -
Popov, I. et al. (2001) The effect of an anti-HLA-B27 immune response on CTL recognition of Chlamydia.
J Immunol. 167 (6): 3375-82. -
Hashimoto, Y. et al. (2003) Two major histocompatibility complex class I-restricted epitopes of the Borna disease virus p10 protein identified by cytotoxic T lymphocytes induced by DNA-based immunization.
J Virol. 77: 6076-81. -
Bradl, M. et al. (2005) Complementary contribution of CD4 and CD8 T lymphocytes to T-cell infiltration of the intact and the degenerative spinal cord.
Am J Pathol. 166: 1441-50.
View The Latest Product References
-
Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.
Immunology. 121: 555-64 -
King, G.D. et al. (2008) Flt3L in combination with HSV1-TK-mediated gene therapy reverses brain tumor-induced behavioral deficits.
Mol Ther. 16: 682-90 -
Bode, U. et al. (2008) Dendritic cell subsets in lymph nodes are characterized by the specific draining area and influence the phenotype and fate of primed T cells.
Immunology. 123: 480-90. -
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35. -
Sanchez-Guajardo, V. (2010) Microglia acquire distinct activation profiles depending on the degree of alpha-synuclein neuropathology in a rAAV based model of Parkinson's disease.
PLoS One. 5: e8784. -
Maenz, M. et al. (2011) A comprehensive flow-cytometric analysis of graft infiltrating lymphocytes, draining lymph nodes and serum during the rejection phase in a fully allogeneic rat cornea transplant model.
Mol Vis. 2011 Feb 8;17:420-9. -
Arndt, T. et al. (2013) A variable CD3⁺ T-cell frequency in peripheral blood lymphocytes associated with type 1 diabetes mellitus development in the LEW.1AR1-iddm rat.
PLoS One. 8 (5): e64305. -
Granados-Durán P et al. (2015) Neuroinflammation induced by intracerebroventricular injection of microbial neuraminidase.
Front Med (Lausanne). 2: 14. -
Nuccitelli R et al. (2015) Nanoelectroablation of Murine Tumors Triggers a CD8-Dependent Inhibition of Secondary Tumor Growth.
PLoS One. 10 (7): e0134364. -
Zhang, Z.M. et al. (2016) Lesional accumulation of CD8(+) cells in sciatic nerves of experimental autoimmune neuritis rats.
Neurol Sci. 37 (2): 199-203. -
Pamukcu, O. et al. (2016) Anti-inflammatory role of obestatin in autoimmune myocarditis.
Clin Exp Pharmacol Physiol. 43 (1): 47-55. -
Dabrowska, S. et al. (2019) Human bone marrow mesenchymal stem cell-derived extracellular vesicles attenuate neuroinflammation evoked by focal brain injury in rats.
J Neuroinflammation. 16 (1): 216. -
James, R.E. et al. (2020) Persistent elevation of intrathecal pro-inflammatory cytokines leads to multiple sclerosis-like cortical demyelination and neurodegeneration.
Acta Neuropathol Commun. 8 (1): 66. -
Matsuyama, S. et al. (2021) Properties of macrophages and lymphocytes appearing in rat renal fibrosis followed by repeated injection of cisplatin.
J Vet Med Sci. 83 (9): 1435-42. -
Dabrowska, S. et al. (2021) Neuroinflammation evoked by brain injury in a rat model of lacunar infarct.
Exp Neurol. 336: 113531. -
Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
Cell Tissue Res. 386 (3): 617-36. -
Zakerkish, F. et al. (2021) Differential effects of the immunosuppressive calcineurin inhibitors cyclosporine-A and tacrolimus on ovulation in a murine model.
Hum Reprod Open. 2021 (2): hoab012. -
Lane, E.L. et al. (2022) Spontaneous Graft-Induced Dyskinesias Are Independent of 5-HT Neurons and Levodopa Priming in a Model of Parkinson's Disease.
Mov Disord. 37 (3): 613-9. -
Silva, B.A. et al. (2022) Understanding the role of the blood brain barrier and peripheral inflammation on behavior and pathology on ongoing confined cortical lesions.
Mult Scler Relat Disord. 57: 103346. -
Hoff, U. et al. (2022) The mTOR inhibitor Rapamycin protects from premature cellular senescence early after experimental kidney transplantation.
PLoS One. 17 (4): e0266319. -
Gad, R.A. et al. (2022) Mitigating effects of Passiflora incarnata. on oxidative stress and neuroinflammation in case of pilocarpine-Induced status epilepticus model
J King Saud Uni - Science. 34 (3): 101886. -
Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis.
Biomedicines. 10 (5): 1021. -
Du, K. et al. (2023) Pathogenesis of selective damage of granule cell layer in cerebellum of rats exposed to methylmercury
J Toxicol Sci 48 (7): 429-39. -
Santos Filho, L.E.D. et al. (2023) Dietary Soy Isoflavones Prevent Metabolic Disturbs Associated with a Deleterious Combination of Obesity and Menopause.
J Med Food. 26 (2): 104-13. -
Midavaine, É. et al. (2024) Discovery of a CCR2-targeting pepducin therapy for chronic pain.
Pharmacol Res. : 107242. -
James, R.B. et al. (2022) Lymphotoxin-alpha expression in the meninges causes lymphoid tissue formation and neurodegeneration.
Brain. 145 (12): 4287-307. -
Ménoret, S. et al. (2020) In Vivo Analysis of Human Immune Responses in Immunodeficient Rats.
Transplantation. 104 (4): 715-23.
- RRID
- AB_324902
- UniProt
- P07725
- Entrez Gene
- Cd8a
- GO Terms
- GO:0016021 integral to membrane
- GO:0006954 inflammatory response
- GO:0019901 protein kinase binding
MCA48A647
1710
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