CD54 antibody | 1A29
Mouse anti Rat CD54 antibody, clone 1A29 used for the evaluation of CD54 expression on rat mesenteric venules by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
In vivo leukocyte trafficking in rat mesenteric post-capillary venules.
Animals were i.p. injected with 10 mL of PBS alone (C) or supplemented with D-glucose (DG; 40 mg/kg), either in the absence or presence of IL-1β (200 ng/kg). L-glucose (LG; 40 mg/kg) was used as an osmotic control. After 18 h, (A) leukocyte rolling flux, (B) leukocyte rolling velocity, (C) leukocyte adhesion and (D) leukocyte migration were determined by intravital microscopy. *P<0.05 versus matched group without IL-1β †P<0.05 versus IL-1β in PBS. Panel E shows representative photomicrographs showing ICAM-1 and VCAM-1 immunolocalization and leukocyte adhesion and transmigration in post-capillary venules. The brown reaction product indicates positive staining. Adhered and transmigrated leukocytes are marked with arrows in the right column. All panels are lightly counterstained with hematoxylin (x400).
From: Azcutia V, Abu-Taha M, Romacho T, Vázquez-Bella M, Matesanz N, Luscinskas FW, et al. (2010)
Inflammation Determines the Pro-Adhesive Properties of High Extracellular D-Glucose in Human Endothelial Cells In Vitro and Rat Microvessels In Vivo.
PLoS ONE 5(4): e10091.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti RatCD54 antibody, clone 1A29 used for the evaluation of ICAM-1 expression in rat corneal tissue by immunofluorescence.
Image caption:
Double immunostaining with anti-CD54 ICAM-1 (in green) and with propidium iodide (in red) on corneas. The corneas were analyzed with a confocal microscope with a 200 X enlargement. Immunofluorescence of ICAM-1 is very weak in control animals (A) and was increased 24 h after the LPS injection (B). Some polymorphonuclear cells (arrow) are visible on the endothelium. Double immunofluorescence with IgG1 mouse antibody for isotypic control (revealed by secondary Alexa Fluor in green) and with propidium iodide (in red) was performed in EIU (C) with a 250X enlargement. Intensities of staining were the same for each assay.
From: Trinh L, Brignole-Baudouin F, Labbé A, Raphaël M, Bourges JL, Baudouin C.
The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 2008 Jun 16;14:1149-56.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD54 antibody, clone 1A29 used for the evaluation of ICAM-1 expression in brain tissue by western blotting in a focal cerebral ischemia model.
Image caption:
Western blot analysis of phosphorylated ERK1/2 and ICAM-1. (a) Phosphorylation of ERK1/2 was significantly higher in hypothermic (33) brains than normothermic (37) ones at 15, 30, and 120 minutes after ischemic insult (n = 6 per group). Total ERK1/2 level was not changed. (b) Ischemia-induced increase of ICAM-1 at 24 hours after MCAO was suppressed by hypothermia. But this suppression was not observed when ERK1/2 inhibition was done (U0126, n = 4) at the dose which almost completely inhibited ERK1/2 phosphorylation. *P < .05 versus normothermia.
From: Choi JS, Park J, Suk K, Moon C, Park YK, Han HS.
Mild Hypothermia Attenuates Intercellular Adhesion Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal Cerebral Ischemia Model.
Stroke Res Treat. 2011;2011:846716.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD54 antibody, clone 1A29 used for the evaluation of ICAM-1 expression in rat brain by immunohistochemistry on formalin fixed cryosections along with activated microglial presence using Mouse anti Rat CD11b antibody, clone OX-42 (MCA275R).
Image caption:
Evaluation of the effects of the PPAR-α agonist fenofibrate administered during the acute phase of experimental stoke in combination with thrombolysis with tPA on neuronal and vascular compartments. Evaluation takes place 72 h after the induction of middle cerebral artery occlusion in the rat. Fenofibrate (50 mg/kg/day) or vehicle was administered by twice-daily gavage over 72 h. Values are means ± SEM.
(d) Expression of ICAM-1 adhesion protein, a marker of leukocyte/endothelium interactions, was increased during ischemia and thrombolysis but was lowered by fenofibrate treatment ( per group; ); scale bar, 100 μm.
f) Expression of CD11b by Ox 42 antibody, a marker of microglial activation, was increased during ischemia and thrombolysis but was lowered by fenofibrate treatment ( per group; ); scale bar, 25 μm.
From: Gautier, S et al.
PPAR-Alpha Agonist Used at the Acute Phase of Experimental Ischemic Stroke Reduces Occurrence of Thrombolysis-Induced Hemorrhage in Rats.
PPAR Research, vol. 2015, Article ID 246329, 6 pages, 2015.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD54 antibody, clone 1A29 (MCA773) used for the evaluation of ICAM-1 expression in rat corneal grafts by immunofluorescence.
Image caption:
Immunohistochemistry staining for CD4+, CD8+, CD11c+, CD163+, CD54+, and CD25+ cells at graft-host junction for different groups. The grafts treated with the PA microfilms or PA eye drops had less dense and diffuse cell infiltration in comparison with the allogeneic control grafts. The syngeneic grafts were nearly free of immunologic cell infiltration. G: Graft. H: Host. Original magnification ×100. Scale bar: 100 μm.
From: Liu Y-C, Peng Y, Lwin NC, Venkatraman SS, Wong TT, Mehta JS (2013)
A Biodegradable, Sustained-Released, Prednisolone Acetate Microfilm Drug Delivery System Effectively Prolongs Corneal Allograft Survival in the Rat Keratoplasty Model.
PLoS ONE 8(8): e70419.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD54 antibody, clone 1A29 (MCA773) used for the evaluation of ICAM-1 expression in rat corneal grafts by immunofluorescence.
Image caption:
Immunohistochemistry staining for CD4+, CD8+, CD11c+, CD163+, CD54+, and CD25+ cells at central grafts for different groups. The syngeneic grafts were nearly free of immunologic cell infiltration. The grafts treated with the PA microfilms or PA eye drops had less dense and diffuse cell infiltration in comparison with the allogeneic control grafts. G: Graft. H: Host. Original magnification ×100. Scale bar: 100 μm.
From: Liu Y-C, Peng Y, Lwin NC, Venkatraman SS, Wong TT, Mehta JS (2013)
A Biodegradable, Sustained-Released, Prednisolone Acetate Microfilm Drug Delivery System Effectively Prolongs Corneal Allograft Survival in the Rat Keratoplasty Model.
PLoS ONE 8(8): e70419.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD54 antibody, clone 1A29 (MCA773) used for the demonstration of ICAM-1 expressing cells in rat epicardial tissue sections by immunofluorescence.
Image caption:
Improved donor cell survival by epicardial PM-MSC coating. (b–d). Immunofluorescence demonstrated that the PM-MSC complex (orange; labelled with CM-DiI) covered the surface of LV walls (both peri-infarct and infarct areas) at 1 h (b), Day 3 (c), and Day 28 (d). The majority of surviving MSCs retained on the heart surface. Scale bar = 1 mm (e–g). Immunofluorescence demonstrated that donor cells in the IM-MSC group were found forming isolated cell-clusters (orange; labelled with CM-DiI) at 1 h (e), Day 3 (f), and Day 28 (g). The surviving donor cell numbers in the IM-MSC group appeared to be smaller compared to the PM-MSC group. Scale bar = 1 mm (h–j). Immunolabeling detected a monolayer of epicardial cells (green) on the surface of heart in the Sham group (h). The epicardial cells disappeared by one day after PM-MSC placement (i). There were sprouting vessels (green) from the heart into the PM-MSC complex by Day 3 after placement (j). Scale bar = 50μm.
From: Ichihara Y, Kaneko M, Yamahara K, Koulouroudias M, Sato N, Uppal R, Yamazaki K, Saito S, Suzuki K.
Self-assembling peptide hydrogel enables instant epicardial coating of the heart with mesenchymal stromal cells for the treatment of heart failure.
Biomaterials. 2018 Feb;154:12-23.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Rat CD54:Alexa Fluor® 647
Mouse anti Rat CD54:FITC
Mouse anti Rat CD54:Low Endotoxin
Mouse anti Rat CD54
- Product Type
- Monoclonal Antibody
- Clone
- 1A29
- Isotype
- IgG1
Mouse anti Rat CD54 antibody, clone 1A29 recognizes the rat CD54 cell surface antigen, also known as intercellular adhesion molecule-1 (ICAM-1), a ~90 kDa adhesion molecule belonging to the immunoglobulin superfamily.
CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (Etienne et al.).
Functionally Mouse anti Rat CD54 antibody, clone 1A29 inhibits homotypic aggregation of PHA blasts.
CD54 is a cell surface ligand of the lymphocyte integrin, LFA-1 and is known to play an important role in various cell-cell interactions in the immune system. Studies suggest that cross-linking of ICAM-1 using clone 1A29 induces calcium signalling (Etienne et al.).
Functionally Mouse anti Rat CD54 antibody, clone 1A29 inhibits homotypic aggregation of PHA blasts.
Product Details
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- Rat Ax cells (a HEV derived cell line).
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- Pack Size: 0.1 mgIgG concentration 0.1 mg/mlPack Size: 0.5 mgIgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.Pack Size: 0.2 mgStore at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
- Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- Q00238 Related reagents
- Entrez Gene
- Icam1 Related reagents
- GO Terms
- GO:0001541 ovarian follicle development
- GO:0001975 response to amphetamine
- GO:0016021 integral to membrane
- GO:0005178 integrin binding
- GO:0005615 extracellular space
- GO:0007569 cell aging
- GO:0008360 regulation of cell shape
- GO:0042493 response to drug
- GO:0010212 response to ionizing radiation
- GO:0010477 response to sulfur dioxide
- GO:0014070 response to organic cyclic compound
- GO:0031669 cellular response to nutrient levels
- GO:0034698 response to gonadotropin stimulus
- GO:0043200 response to amino acid stimulus
- GO:0043498 cell surface binding
- GO:0046688 response to copper ion
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045471 response to ethanol
- GO:0045907 positive regulation of vasoconstriction
- GO:0050900 leukocyte migration
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051926 negative regulation of calcium ion transport
- GO:0071222 cellular response to lipopolysaccharide
- GO:0071312 cellular response to alkaloid
- GO:0071333 cellular response to glucose stimulus
- GO:0071347 cellular response to interleukin-1
- GO:0071356 cellular response to tumor necrosis factor
- GO:0071456 cellular response to hypoxia
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD54 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/25 | Neat Pack Size: 0.1 mg 1/100 Pack Size: 0.5 mg |
|
| Flow Cytometry | 1/25 | 1/50 | |
| Functional Assays | |||
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Western Blotting | |||
| Flow Cytometry | 1/25 | 1/50 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin 1 | |||
| Immunoprecipitation | |||
| Western Blotting |
- 1This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B for this purpose.
- 1This clone is suitable for use on paraffin embedded material using target unmasking fluid HIS003B for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | 100 Tests/1ml | F | ||
| Mouse IgG1 Negative Control:FITC | MCA1209F | 0.1 mg | F | ||
| Mouse IgG1 Negative Control:Low Endotoxin | MCA1209EL | 0.5 mg | F | ||
| Mouse IgG1 Negative Control | MCA1209 | 0.1 mg | F |
Application Based External Images
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Product Specific References
References for CD54 antibody
-
Kawai, T. et al. (1999) Selective diapedesis of Th1 cells induced by endothelial cell RANTES.
J Immunol. 163: 3269-78. -
Sato, N. et al. (2000) Roles of ICAM-1 for abnormal leukocyte recruitment in the microcirculation of bleomycin-induced fibrotic lung injury.
Am J Respir Crit Care Med. 161: 1681-8. -
Etienne, S. et al. (1998) ICAM-1 signaling pathways associated with Rho activation in microvascular brain endothelial cells.
J Immunol. 161 (10): 5755-61. -
Etienne-Manneville, S. et al. (2000) ICAM-1-coupled cytoskeletal rearrangements and transendothelial lymphocyte migration involve intracellular calcium signaling in brain endothelial cell lines.
J Immunol. 165 (6): 3375-83. -
McKechnie, N. M. et al. (2002) Antigenic mimicry: Onchocerca volvulus antigen-specific T cells and ocular inflammation.
Invest Ophthalmol Vis Sci. 43:411-8. -
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 2003 Dec;87(12):1515-22. -
Kielian, T. et al. (2000) Proinflammatory cytokine, chemokine, and cellular adhesion molecule expression during the acute phase of experimental brain abscess development.
Am J Pathol. 157: 647-58. -
Trinh, L. et al. (2008) The corneal endothelium in an endotoxin-induced uveitis model: correlation between in vivo confocal microscopy and immunohistochemistry.
Mol Vis. 14: 1149-56. -
Adamson, P. et al. (1999) Lymphocyte migration through brain endothelial cell monolayers involves signaling through endothelial ICAM-1 via a rho-dependent pathway.
J Immunol. 162: 2964-73. -
Arsenović-Ranin, N. et al. (2000) A monoclonal antibody to the rat Crry/p65 antigen, a complement regulatory membrane protein, stimulates adhesion and proliferation of thymocytes.
Immunology. 100: 334-44. -
Beck-Schimmer, B. et al. (2001) Hypoxia mediates increased neutrophil and macrophage adhesiveness to alveolar epithelial cells.
Am J Respir Cell Mol Biol. 25: 780-7. -
Couty, J.P. (2007) PECAM-1 engagement counteracts ICAM-1-induced signaling in brain vascular endothelial cells.
J Neurochem. 103: 793-801. -
Deng, H. et al. (2003) Mild hypothermia inhibits inflammation after experimental stroke and brain inflammation.
Stroke. 34: 2495-501. -
Ikezumi, Y. et al. (2004) Macrophage-mediated renal injury is dependent on signaling via the JNK pathway.
J Am Soc Nephrol. 15: 1775-84. -
Kanellis, J. et al. (2010) JNK signalling in human and experimental renal ischaemia/reperfusion injury.
Nephrol Dial Transplant. 25: 2898-908. -
Westermann, D. et al. (2007) Cardioprotective and anti-inflammatory effects of interleukin converting enzyme inhibition in experimental diabetic cardiomyopathy.
Diabetes. 56: 1834-41. -
Zhu, X. et al. (2003) Matrine protects sinusoidal endothelial cells from cold ischemia and reperfusion injury in rat orthotopic liver transplantation.
Ann Clin Lab Sci. 33: 216-25. -
Choi, J.S. et al. (2011) Mild Hypothermia Attenuates Intercellular Adhesion Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal Cerebral Ischemia Model.
Stroke Res Treat. 2011: 846716. -
Azcutia V et al. (2010) Inflammation determines the pro-adhesive properties of high extracellular d-glucose in human endothelial cells in vitro and rat microvessels in vivo.
PLoS One. 5 (4): e10091. -
Li, W. & Klein, S.L. (2012) Seoul virus-infected rat lung endothelial cells and alveolar macrophages differ in their ability to support virus replication and induce regulatory T cell phenotypes.
J Virol. 86 (21): 11845-55. -
Gates, D. et al. (2012) Apo J/clusterin expression and secretion: evidence for 15-deoxy-Δ(12,14)-PGJ(2)-dependent mechanism.
Biochim Biophys Acta. 1821 (2): 335-42. -
Li, Z. et al. (2015) Three-dimensional graphene foams loaded with bone marrow derived mesenchymal stem cells promote skin wound healing with reduced scarring.
Mater Sci Eng C Mater Biol Appl. 57: 181-8. -
Gautier, S. et al. (2015) PPAR-Alpha Agonist Used at the Acute Phase of Experimental Ischemic Stroke Reduces Occurrence of Thrombolysis-Induced Hemorrhage in Rats.
PPAR Research. 2015: 1-6. -
Liu, Y.C. et al. (2013) A biodegradable, sustained-released, prednisolone acetate microfilm drug delivery system effectively prolongs corneal allograft survival in the rat keratoplasty model.
PLoS One. 8 (8): e70419. -
Ichihara, Y. et al. (2018) Self-assembling peptide hydrogel enables instant epicardial coating of the heart with mesenchymal stromal cells for the treatment of heart failure.
Biomaterials. 154: 12-23.
