Browsing Modes for Business Users

CD44 antibody | OX-50

ISO 9001 Quality Assurance Manufacturing | Print
Anti Rat CD44 Antibody, clone OX-50 thumbnail image 1 Anti Rat CD44 Antibody, clone OX-50 thumbnail image 2 Anti Rat CD44 Antibody, clone OX-50 thumbnail image 3 Anti Rat CD44 Antibody, clone OX-50 thumbnail image 4 Anti Rat CD44 Antibody, clone OX-50 thumbnail image 5 Anti Rat CD44 Antibody, clone OX-50 thumbnail image 6 Anti Rat CD44 Antibody, clone OX-50 thumbnail image 7
Anti Rat CD44 Antibody, clone OX-50 gallery image 1
Figure A. Alexa Fluor 647 conjugated Mouse anti Rat CD4 (MCA153A647) and RPE conjugated Mouse IgG1 isotype control (MCA1209PE). Figure B. Alexa Fluor 647 conjugated Mouse anti Rat CD4 (MCA153A647) and RPE conjugated Mouse anti Rat CD44 (MCA643PE). All experiments performed on red cell lysed rat blood gated on lymphoid cells in the presence of 10% rat serum. Data acquired on the ZE5 Cell Analyzer.
Anti Rat CD44 Antibody, clone OX-50 gallery image 2
Figure A. Alexa Fluor 647 conjugated Mouse anti Rat CD4 (MCA153A647) and RPE conjugated Mouse IgG2a isotype control (MCA1210PE). Figure B. Alexa Fluor647 conjugated Mouse anti Rat CD4 (MCA153A647) and RPE conjugated Mouse anti Rat CD44 (MCA643PE). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD44 Antibody, clone OX-50 gallery image 3
Figure A. RPE conjugated Mouse anti Rat CD44 (MCA643PE) and Alexa Fluor 647 conjugated Mouse IgG2a isotype control (MCA1210A647). Figure B. RPE conjugated Mouse anti Rat CD44 (MCA643PE) and RPE conjugated Mouse anti Rat CD4 (MCA153A647). All experiments performed on red cell lysed rat blood gated on mononuclear cells.
Anti Rat CD44 Antibody, clone OX-50 gallery image 4
Published customer image:
FITC conjugated Mouse anti Rat CD44 antibody, clone OX-50 (MCA643F) used for the phenotyping of rat mesenchymal stem cells by flow cytometry.
Image caption:
Subcultured mesenchymal stem cells (MSCs) in vitro. A: Phase contrast micrographs of mesenchymal stem cells cultured on day seven (40×). B: Immunofluorescence staining of CD29 was positive. C: Flow cytometric analysis showed that the positive rates of CD29, CD34, CD44, CD45, CD71, CD90, and CD133 were 81.56%, 0.10%, 88.77%, 0.17%, 10.02%, 98.43%, and 1.56%, respectively. D: Scanning electron microscopy (SEM) of MSCs showed a long spindle-shaped appearance with rich processes (1000×).

From: Jiang TS, Cai L, Ji WY, Hui YN, Wang YS, Hu D, Zhu J.
Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats.
Mol Vis. 2010 Jul 14;16:1304-16.
Anti Rat CD44 Antibody, clone OX-50 gallery image 5
Published customer image:
Fitc conjugated Mouse anti Rat CD44 antibody, clone OX-50 (MCA643F) used to assess CD31 expression on putitive rat adipose derived stem cells (ADSCs) by flow cytometry.
Image caption:
Identification of ADSCs and assessment of their plating efficiency. A. Flow cytometry analysis showed that surface markers including CD44, CD73 and CD90 were positive. While CD45, CD11b and CD31 were negative.

From: Aminzadeh A, Tekiyeh Maroof N, Mehrabani M, Bahrampour Juybari K, Sharifi AM.
Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.
Cell J. 2020 Jul;22(Suppl 1):38-48.
doi: 10.22074/cellj.2020.6958.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat CD44 Antibody, clone OX-50 gallery image 6
Published customer image:
Mouse anti Rat CD44 antibody, clone OX-50 (MCA643R) used to evbaluate CD44 expression in Rat kidney by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative pictures of immunohistochemical stained sections for CD44, a marker for MSC, in ten groups of rats show CD44 positive stains in the glomeruli indicated by arrows in MSCs or HMSCs treated kidneys compared to control kidney (A1–10). Anti-Thy1.1 evoked mesangial lysis (indicated by +) and the mesangial matrix accumulation (indicated with *) in the nephritic rats.

From: Chang HH, Hsu SP, Chien CT.
Intrarenal Transplantation of Hypoxic Preconditioned Mesenchymal Stem Cells Improves Glomerulonephritis through Anti-Oxidation, Anti-ER Stress, Anti-Inflammation, Anti-Apoptosis, and Anti-Autophagy.
Antioxidants (Basel). 2019 Dec 18;9(1):2.
doi: 10.3390/antiox9010002.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Rat CD44 Antibody, clone OX-50 gallery image 7
Published customer image:
Mouse anti Rat CD44 antibody, clone OX=50 (MCA643GA) used to assess CD44 expression in rat kidney lysates by western blotting.
Image caption:
Western blot analysis of CD 44 expression of kidney homogenates in four groups of kidneys.

From: Cheng YH, Chen KH, Sung YT, Yang CC, Chien CT.
Intrarenal Arterial Transplantation of Dexmedetomidine Preconditioning Adipose Stem-Cell-Derived Microvesicles Confers Further Therapeutic Potential to Attenuate Renal Ischemia/Reperfusion Injury through miR-122-5p/Erythropoietin/Apoptosis Axis.
Antioxidants (Basel). 2022 Aug 30;11(9):1702.
doi: 10.3390/antiox11091702.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Rat CD44:RPE

Product Type
Monoclonal Antibody
Clone
OX-50
Isotype
IgG1
Specificity
CD44

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA643PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Mouse anti Rat CD44 antibody, clone OX-50 recognizes the rat CD44 cell surface antigen, also known as Extracellular matrix receptor III, P90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Phagocytic glycoprotein 1, PGP-1 or Phagocytic glycoprotein I.

CD44 is a 482 amino acid ~85 kDa single pass type I transmembrane glycoprotein, expressed by T cells, B cells, macrophages and thymocytes, with expression being increased following activation.

Target Species
Rat
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Rat T cell blasts.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS0/1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests
>
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

References for CD44 antibody

  1. Stevenson, K.S. et al. (2009) Isolation, characterization, and differentiation of thy1.1-sorted pancreatic adult progenitor cell populations.
    Stem Cells Dev. 18:1389-98.
  2. Jiang, T.S. et al. (2010) Reconstruction of the corneal epithelium with induced marrow mesenchymal stem cells in rats.
    Mol Vis. 16: 1304-16.
  3. Kanellis, J. et al. (2010) JNK signalling in human and experimental renal ischaemia/reperfusion injury.
    Nephrol Dial Transplant. 25: 2898-908.
  4. Li, S. et al. (2010) Upregulation of CXCR4 favoring neural-like cells migration via AKT activation.
    Neurosci Res. 67: 293-9.
  5. Stephens, L.A. et al. (2004) Phenotypic characterization of regulatory CD4+CD25+ T cells in rats.
    Int Immunol. 16: 365-75.
  6. Rice, C.M. et al. (2010) Multipotent adult progenitor cell isolation and proliferation in cytokine and serum-free medium conditioned by rat B104 cells.
    Br J Haematol. 148: 441-4.
  7. Carulli, D. et al (2006) Composition of perineuronal nets in the adult rat cerebellum and the cellular origin of their components.
    J Comp Neurol. 494: 559-77.
  8. Galtrey, C.M. et al. (2008) Distribution and synthesis of extracellular matrix proteoglycans, hyaluronan, link proteins and tenascin-R in the rat spinal cord.
    Eur J Neurosci. 27: 1373-90.
    9. View The Latest Product References
  9. Hofmann, N. et al. (2002) Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats.
    BMC Immunol. 3: 11.
  10. Walther, M. et al. (2001) Exogenous antigen containing perivascular phagocytes induce a non-encephalitogenic extravasation of primed lymphocytes.
    J Neuroimmunol. 117: 30-42.
  11. Suzuki, A. et al. (2006) Localization of CD44 and hyaluronan in the synovial membrane of the rat temporomandibular joint.
    Anat Rec A Discov Mol Cell Evol Biol. 288: 646-52.
  12. Goransson, V. et al. (2004) Renal hyaluronan accumulation and hyaluronan synthase expression after ischaemia-reperfusion injury in the rat.
    Nephrol Dial Transplant.19: 823-30.
  13. Campbell, N.G. et al. (2016) Cell Size Critically Determines Initial Retention of Bone Marrow Mononuclear Cells in the Heart after Intracoronary Injection: Evidence from a Rat Model.
    PLoS One. 11 (7): e0158232.
  14. Bejar, M.T. et al. (2016) Inhibition of Notch rescues the angiogenic potential impaired by cardiovascular risk factors in epicardial adipose stem cells.
    FASEB J. 30 (8): 2849-59.
  15. Rochefort, G.Y. et al. (2006) Multipotential mesenchymal stem cells are mobilized into peripheral blood by hypoxia.
    Stem Cells. 24 (10): 2202-8.
  16. Redondo, J. et al. (2015) Reductions in kinesin expression are associated with nitric oxide-induced axonal damage.
    J Neurosci Res. 93 (6): 882-92.
  17. Huang, X. et al. (2019) MRI Tracking of SPIO- and Fth1-Labeled Bone Marrow Mesenchymal Stromal Cell Transplantation for Treatment of Stroke.
    Contrast Media Mol Imaging. 2019: 5184105.
  18. Aminzadeh, A. et al. (2020) Investigating The Alterations of Oxidative Stress Status, Antioxidant Defense Mechanisms, MAP Kinase and Mitochondrial Apoptotic Pathway in Adipose-Derived Mesenchymal Stem Cells from STZ Diabetic Rats.
    Cell J. 22 (Suppl 1): 38-48.
  19. Paiva, R.G. et al. (2020) Stem cells in end-to-side neurorrhaphy. Experimental study in rats
    Acta Cirúrgica Brasileira. 35 (12) [Epub ahead of print].
  20. Hou, B. et al. (2018) Comparison of the Effects of BMSC-derived Schwann Cells and Autologous Schwann Cells on Remyelination Using a Rat Sciatic Nerve Defect Model.
    Int J Biol Sci. 14 (13): 1910-22.
  21. Porwal, K. et al.kfz172 (2019) Increased bone marrow-specific adipogenesis by clofazimine causes impaired fracture healing, osteopenia and osteonecrosis without extra-skeletal effects in rats.
    Toxicol Sci. kfz172.
  22. Chang, H.H. et al. (2019) Intrarenal Transplantation of Hypoxic Preconditioned Mesenchymal Stem Cells Improves Glomerulonephritis through Anti-Oxidation, Anti-ER Stress, Anti-Inflammation, Anti-Apoptosis, and Anti-Autophagy.
    Antioxidants (Basel). 9 (1): 2.
  23. Wu, J. et al. (2022) Reinforcing the function of bone graft via the Ca-P ceramics dynamic behavior-enhanced osteogenic microenvironment for optimal bone regeneration and reconstruction
    Applied Materials Today. 27: 101465.
  24. Cheng, Y.H. et al. (2022) Intrarenal Arterial Transplantation of Dexmedetomidine Preconditioning Adipose Stem-Cell-Derived Microvesicles Confers Further Therapeutic Potential to Attenuate Renal Ischemia/Reperfusion Injury through miR-122-5p/Erythropoietin/Apoptosis Axis.
    Antioxidants (Basel). 11(9): 1702.

Synonyms
H-CAM
PGP-1
RRID
AB_321723
UniProt
P26051
Entrez Gene
Cd44
GO Terms
GO:0001558 regulation of cell growth
GO:0001955 blood vessel maturation
GO:0007155 cell adhesion
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005540 hyaluronic acid binding
GO:0005634 nucleus
GO:0006954 inflammatory response
GO:0014070 response to organic cyclic compound
GO:0016323 basolateral plasma membrane
GO:0016324 apical plasma membrane
GO:0016477 cell migration
GO:0019901 protein kinase binding
GO:0031175 neuron projection development
GO:0033031 positive regulation of neutrophil apoptosis
GO:0033189 response to vitamin A
GO:0034238 macrophage fusion
GO:0043234 protein complex
GO:0051219 phosphoprotein binding

MCA643PE

156540 162161 INN1608

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD44 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Rat Products
Please note