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CD43 antibody | W3/13

Mouse anti Rat CD43:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price

Mouse anti Rat CD43 antibody, clone W3/13 recognizes the rat CD43 cell surface antigen, also known as leukosialin, sialophorin or W3/13 antigen. CD43 is a 371 amino acid ~95 kDa heavily glycosylated single pass type 1 transmembrane glycoprotein (Killeen et al. 1987) expressed by all leucocytes with the exception of B lymphocytes. CD43, in mice acts as a T-cell counter-receptor for CD169 (Siglec-1) suggesting a role in cell-cell interactions (van den Berg et al. 2001)

Mouse anti Rat CD43 antibody, clone W3/13 is routinely tested in flow cytometry on rat splenocytes.

Target Species
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Rat thymocyte membrane glycoproteins.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
List Price Your Price
Description Mouse IgG1 Negative Control:FITC

References for CD43 antibody

  1. Brown, W.R.A. et al. (1981) Identification of a glycophorin-like molecule at the cell surface of rat thymocytes.
    Nature. 289: 456-460.
  2. Barclay, A. N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42: 593-600
  3. Forbes, R. D. C. et al. (1983) Leukocyte subsets in first-set rat cardiac allograft rejection. A serial immunohistologic study using monoclonal antibodies
    Transplantation. 36: 681-686
  4. Jung, S. et al. (1994) Therapeutic effect of transforming growth factor-beta 2 on actively induced EAN but not adoptive transfer EAN
    Immunology. 83: 545-551.
  5. Bataller, R. et al. (2003) Prolonged infusion of angiotensin II into normal rats induces stellate cell activation and proinflammatory events in liver.
    Am J Physiol Gastrointest Liver Physiol. 285: G642-651
  6. Rice, E.K. et al. (2003) Induction of MIF synthesis and secretion by tubular epithelial cells: a novel action of angiotensin II.
    Kidney Int. 63 (4): 1265-75.
  7. Schwab, J.M. et al. (2005) Spinal cord injury induces early and persistent lesional P2X4 receptor expression.
    J Neuroimmunol. 163: 185-9.
  8. Conrad, S. et al. (2005) Prolonged lesional expression of RhoA and RhoB following spinal cord injury.
    J Comp Neurol. 487: 166-75.
  9. View The Latest Product References
  10. Zhang, Z. et al. (2008) FTY720 ameliorates experimental autoimmune neuritis by inhibition of lymphocyte and monocyte infiltration into peripheral nerves.
    Exp Neurol. 210: 681-90.
  11. Yamanaka, Y. et al. (2011) Immunohistochemical analysis of subcutaneous tissue reactions to methacrylate resin-based root canal sealers.
    Int Endod J. 44: 669-75.
  12. Dort, J. et al. (2012) Beneficial effects of cod protein on skeletal muscle repair following injury.
    Appl Physiol Nutr Metab. 37 (3): 489-98.
  13. Duchesne, E. et al. (2013) Mast cells can regulate skeletal muscle cell proliferation by multiple mechanisms.
    Muscle Nerve. 48 (3): 403-14.
  14. Xu, K. et al. (2016) Expression of aryl hydrocarbon receptor in rat brain lesions following traumatic brain injury.
    Diagn Pathol. 11 (1): 72.
  15. Dort, J. et al. (2016) Shrimp Protein Hydrolysate Modulates the Timing of Proinflammatory Macrophages in Bupivacaine-Injured Skeletal Muscles in Rats.
    Biomed Res Int. 2016: 5214561.
  16. Zhang, Z.M. et al. (2016) Lesional accumulation of CD8(+) cells in sciatic nerves of experimental autoimmune neuritis rats.
    Neurol Sci. 37 (2): 199-203.
  17. Ornellas, F.M. et al. (2019) Mesenchymal Stromal Cells Induce Podocyte Protection in the Puromycin Injury Model.
    Sci Rep. 9 (1): 19604.
  18. Grad, E. et al. (2019) Monocyte Modulation by Liposomal Alendronate Improves Cardiac Healing in a Rat Model of Myocardial Infarction
    Regenerative Engineering and Translational Medicine. 5 (3): 280-9.
  19. Kami, K. et al. (2019) Role of 72-kDa Heat Shock Protein in Heat-stimulated Regeneration of Injured Muscle in Rat.
    J Histochem Cytochem. 67 (11): 791-9.
  20. Cąkała-Jakimowicz, M. & Puzianowska-Kuznicka, M. (2022) Towards Understanding the Lymph Node Response to Skin Infection with Saprophytic Staphylococcus epidermidis..
    Biomedicines. 10 (5): 1021.
  21. Merlini, A. et al. (2022) Distinct roles of the meningeal layers in CNS autoimmunity.
    Nat Neurosci. 25 (7): 887-99.

Entrez Gene
GO Terms
GO:0002544 chronic inflammatory response
GO:0016021 integral to membrane
GO:0005902 microvillus
GO:0009986 cell surface
GO:0019904 protein domain specific binding
GO:0032154 cleavage furrow
GO:0042117 monocyte activation
GO:0071594 thymocyte aggregation


0812R 155974

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