CD172a antibody | ED9
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Mouse anti Rat CD172a:RPE
- Product Type
- Monoclonal Antibody
|Mouse anti Rat CD172a antibody, clone ED9 recognizes rat Tyrosine-protein phosphatase non-receptor type substrate 1, also known as CD172a, Signal-regulatory protein alpha-1, SIRPα -1, SHP substrate 1, Macrophage membrane protein MFP150 or Macrophage fusion receptor. CD172a is a 509 amino acid ~56 kDa single pass type 1 transmembrane glycoprotein expressed selectively by myeloid cells and by neurons (UniProt: P97710). Mouse anti Rat CD172a antibody, clone ED9 has been reported to bind to an alternative epitpe to another anti CD172 antibody, clone OX-41 (Adams et al. 1998) and has been reported to block the interaction of CD172a - CD47 (de Vries et al. 2002).|
- Target Species
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitute with 1 ml distilled water
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose
- Spleen cell homogenate.
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the Sp2/0 Ag-14 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578
- For research purposes only
- 12 months from date of despatch
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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|Description||Product Code||Applications||Pack Size||List Price||Your Price||Quantity|
|Mouse IgG1 Negative Control:RPE||MCA1209PE||F||100 Tests||Log in|
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|Description||Mouse IgG1 Negative Control:RPE|
References for CD172a antibody
Damoiseaux, J.G. et al. (1989) Rat bone marrow and monocyte cultures: influence of culture time and lymphokines on the expression of macrophage differentiation antigens.
J Leukoc Biol. 46 (3): 246-53.
Damoiseaux, J.G. et al. (1989) Heterogeneity of macrophages in the rat evidenced by variability in determinants: two new anti-rat macrophage antibodies against a heterodimer of 160 and 95 kd (CD11/CD18).
J Leukoc Biol. 46 (6): 556-64.
Adams, S. et al. (1998) Signal-regulatory protein is selectively expressed by myeloid and neuronal cells.
J Immunol. 161 (4): 1853-9.
DeVries, H.E. et al. (2002) Signal-regulatory protein alpha-CD47 interactions are required for the transmigration of monocytes across cerebral endothelium.
J Immunol. 168 (11): 5832-9.
Blackbeard, J. et al. (2007) Quantification of the rat spinal microglial response to peripheral nerve injury as revealed by immunohistochemical image analysis and flow cytometry.
J Neurosci Methods. 164 (2): 207-17.
Bode, U. et al. (2008) Dendritic cell subsets in lymph nodes are characterized by the specific draining area and influence the phenotype and fate of primed T cells.
Immunology. 123 (4): 480-90.
Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92.
Holler, J. et al. (2008) Neuropeptide Y is expressed by rat mononuclear blood leukocytes and strongly down-regulated during inflammation.
J Immunol. 181 (10): 6906-12.
View The Latest Product References
Schmiedl, A. et al. (2021) Lung development and immune status under chronic LPS exposure in rat pups with and without CD26/DPP4 deficiency.
Cell Tissue Res. Oct 04 [Epub ahead of print].
- SIRP ALPHA
- Entrez Gene
- GO Terms
- GO:0016477 cell migration
- GO:0017124 SH3 domain binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"View all Anti-Rat Products
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