Tubulin Beta antibody | TU-13
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Mouse anti Pig Tubulin Beta (N-Terminal)
- Product Type
- Monoclonal Antibody
- Clone
- TU-13
- Isotype
- IgM
- Specificity
- Tubulin Beta
- Region
- (N-TERMINAL)
Antibody Quality and Performance Guarantee
| Mouse anti Pig tubulin beta antibody, clone TU-13 recognizes an epitope within the N-terminal region (NT) of tubulin beta, a ~50 kDa globular protein and major constituent of microtubules within the cytoskeleton, involved in meiosis, mitosis, intracellular transport and maintaining cell shape. The evolutionarily conserved heterodimer tubulin, made up of a tubulin alpha and beta chain, binds two molecules of GTP, one at a non-exchangeable site on the alpha chain and one at an exchangeable site on the beta chain. Only the beta chain possesses GTPase activity however, hydrolysing GTP to GDP, and influencing the stability of the dimer within the microtubule. Mouse anti Pig tubulin beta antibody, clone TU-13 partially inhibits microtubule assembly in vitro (Draber, P. et al.). Mouse anti Pig Tubulin beta antibody, clone TU-13 has also been successfully employed for the demonstration of neuronal cells in mouse brain by immunohistochemistry on floating sections (Nováková et al. 1997). |
- Target Species
- Pig
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Plants Expected from Sequence Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgM - liquid
- Preparation
- Purified IgM prepared by ion exchange chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Tubulin beta from porcine brain.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Immunohistology - Frozen | |
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| Western Blotting | |
1.0ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- MCA2703 detects a band of approximately 50kDa in human RAJI and mouse EL4 cell lysates.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | STAR138A | C E P WB | 1 ml |
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| Description | Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed) | ||||||
| Goat anti Mouse IgG/A/M:Alk. Phos. | STAR87A | C E WB | 1 mg |
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| Description | Goat anti Mouse IgG/A/M:Alk. Phos. | ||||||
| Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | STAR87P | E | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) | ||||||
References for Tubulin Beta antibody
-
Dráber, P. et al. (1990) Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies.
FEBS Lett. 262 (2): 209-11. -
Bataveljić D et al. (2012) Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1(G93A) rat model.
Glia. 60 (12): 1991-2003.
Further Reading
-
Krauhs, E. et al. (1981) Complete amino acid sequence of beta-tubulin from porcine brain.
Proc Natl Acad Sci U S A. 78 (7): 4156-60.
- RRID
- AB_906162
- UniProt
- P02554
- GO Terms
- GO:0005515 protein binding
- GO:0003924 GTPase activity
- GO:0005198 structural molecule activity
- GO:0005525 GTP binding
- GO:0005737 cytoplasm
- GO:0005874 microtubule
- GO:0007018 microtubule-based movement
- GO:0051258 protein polymerization
View more products with TUBULIN specificity
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