Tubulin Beta antibody | TU-13

Mouse anti Pig Tubulin Beta (N-Terminal)

Product Type
Monoclonal Antibody
Clone
TU-13
Isotype
IgM
Specificity
Tubulin Beta
Region
(N-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
MCA2703
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E WB 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Pig tubulin beta antibody, clone TU-13 recognizes an epitope within the N-terminal region (NT) of tubulin beta, a ~50 kDa globular protein and major constituent of microtubules within the cytoskeleton, involved in meiosis, mitosis, intracellular transport and maintaining cell shape.

The evolutionarily conserved heterodimer tubulin, made up of a tubulin alpha and beta chain, binds two molecules of GTP, one at a non-exchangeable site on the alpha chain and one at an exchangeable site on the beta chain. Only the beta chain possesses GTPase activity however, hydrolysing GTP to GDP, and influencing the stability of the dimer within the microtubule.

Mouse anti Pig tubulin beta antibody, clone TU-13 partially inhibits microtubule assembly in vitro (Draber, P. et al.). Mouse anti Pig Tubulin beta antibody, clone TU-13 has also been successfully employed for the demonstration of neuronal cells in mouse brain by immunohistochemistry on floating sections (Nováková et al. 1997).

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Plants Expected from Sequence
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgM - liquid
Preparation
Purified IgM prepared by ion exchange chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Tubulin beta from porcine brain.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Frozen
Western Blotting 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2703 detects a band of approximately 50kDa in human RAJI and mouse EL4 cell lysates.

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References for Tubulin Beta antibody

  1. Dráber, P. et al. (1990) Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies.
    FEBS Lett. 262 (2): 209-11.
  2. Bataveljić D et al. (2012) Changes in the astrocytic aquaporin-4 and inwardly rectifying potassium channel expression in the brain of the amyotrophic lateral sclerosis SOD1(G93A) rat model.
    Glia. 60 (12): 1991-2003.

Further Reading

  1. Krauhs, E. et al. (1981) Complete amino acid sequence of beta-tubulin from porcine brain.
    Proc Natl Acad Sci U S A. 78 (7): 4156-60.

RRID
AB_906162
UniProt
P02554
GO Terms
GO:0005515 protein binding
GO:0003924 GTPase activity
GO:0005198 structural molecule activity
GO:0005525 GTP binding
GO:0005737 cytoplasm
GO:0005874 microtubule
GO:0007018 microtubule-based movement
GO:0051258 protein polymerization
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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