IgA antibody | K61 1B4
Mouse anti Pig IgA antibody, clone K61 1B4 (MCA638GA) used for the measurement of ova specific IgA levels in serum by ELISA.
Image caption:
OVA-specific antibody-mediated immune responses in serum from newborn piglets gavaged with OVA then i.p. immunized with OVA at four weeks of age. Piglets (n = 5/group) were gavaged and i.p. immunized as described in Figure 1. Control newborn piglets were not gavaged or immunized with OVA. We measured serum anti-OVA IgM (A), IgA (B), IgG (C), IgG1 (D) and IgG2 (E) production on day 28, day 42 and day 49 after birth. Each data point represents an individual animal and median values are indicated by horizontal lines. *p <0.05., **p <0.01.
From: Pasternak JA, Ng SH, Buchanan RM, Mertins S, Mutwiri GK, Gerdts V, Wilson HL.
Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 2015 Dec;11(1):350.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig IgA antibody, clone K61 1B4 (MCA638) used for the measurement of ova specific IgA levels in lung lavages by ELISA.
Image caption:
OVA-specific antibody-mediated immune responses in lung washes from newborn piglets gavaged with OVA then i.p. immunized with OVA at four weeks of age. Piglets (n = 5/group) were gavaged and i.p. immunized as described in Figure 1. Control newborn piglets were not gavaged or immunized with OVA. Lung lavages were collected four weeks post i.p. immunization and OVA-specific serum IgM (A), IgA (B), IgG (C), IgG1 (D), and IgG2 (E) titres were measured. ELISA titres are expressed as the reciprocal of the highest dilution resulting in a reading of two standard deviations above the negative control. Each data point represents an individual animal and median values are indicated by horizontal lines. *p <0.05, **p <0.01.
From: Pasternak JA, Ng SH, Buchanan RM, Mertins S, Mutwiri GK, Gerdts V, Wilson HL. Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 2015 Dec;11(1):350.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse Anti Pig IgA antibody, clone K61 1B4 recognizes porcine immunoglobulin (Ig) alpha chain. No cross-reactivity is observed with either porcine IgM or IgG.
It has been suggested that pigs possess two subclasses of IgA, referred to as either IgA1 and IgA2 similar to the human IgA subclasses. More recent research indicates that these are allotypic variants and described as IgAa and IgAb (Navarro et al. 2000). Research undertaken in connection with porcine IgA response to Foot and Mouth disease (Pacheco et al. 2010) has demonstrated that clone K61 1B4 recognises both IgAa and IgAb allotypes.
IgA is the dominant immunoglobulin found in surface secretions where its role is in the protection of body surfaces. High levels of IgA may be detected in tracheal secretions, saliva, intestinal fluid and urogenital tract secretions. The primary function of IgA is to prevent adherence of bacteria and virus to epithelial surfaces. IgA may also act within epithelial cells where it has a role in interrupting viral replication.
It has been suggested that pigs possess two subclasses of IgA, referred to as either IgA1 and IgA2 similar to the human IgA subclasses. More recent research indicates that these are allotypic variants and described as IgAa and IgAb (Navarro et al. 2000). Research undertaken in connection with porcine IgA response to Foot and Mouth disease (Pacheco et al. 2010) has demonstrated that clone K61 1B4 recognises both IgAa and IgAb allotypes.
IgA is the dominant immunoglobulin found in surface secretions where its role is in the protection of body surfaces. High levels of IgA may be detected in tracheal secretions, saliva, intestinal fluid and urogenital tract secretions. The primary function of IgA is to prevent adherence of bacteria and virus to epithelial surfaces. IgA may also act within epithelial cells where it has a role in interrupting viral replication.
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine IgA
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of IgA antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/50K | 1/500K | |
| Flow Cytometry | |||
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- ELISA
- This product may be used as a detection reagent in ELISA applications
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | 0.5 mg | E WB |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti Pig Ig Lambda Light Chain | MCA633GA | 0.1 mg | C E F P | ||
| Mouse anti Pig IgA Secretory Component | MCA634GA | 0.1 mg | E | ||
| Mouse anti Pig IgG1 | MCA635GA | 0.1 mg | E | ||
| Mouse anti Pig IgG2 | MCA636GA | 0.1 mg | E | ||
| Mouse anti Pig IgM | MCA637GA | 0.1 mg | C E F |
Product Specific References
References for IgA antibody
-
Leitão, A. et al. (2001) The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response.
J Gen Virol. 82 (Pt 3): 513-23. -
Bourges, D. et al. (2004) T and IgA B lymphocytes of the pharyngeal and palatine tonsils: differential expression of adhesion molecules and chemokines.
Scand J Immunol. 60 (4): 338-50. -
Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
Parasitology. 136 (6): 691-7. -
Pacheco, J.M. et al. (2010) IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination.
Clin Vaccine Immunol. 17: 550-8. -
Pasternak, J.A. et al. (2015) Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 11 (1): 350. -
Ewaschuk JB et al. (2012) Barley-derived β-glucans increases gut permeability, ex vivo epithelial cell binding to E. coli, and naive T-cell proportions in weanling pigs.
J Anim Sci. 90 (8): 2652-62. -
Inman CF et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
PLoS One. 7 (3): e33707. -
Guzman-Bautista, E.R. et al. (2015) Tracheal and bronchial polymeric immunoglobulin secretory immune system (PISIS) development in a porcine model.
Dev Comp Immunol. 53 (2): 271-82. -
Kringel H et al. (2015) Serum antibody responses in pigs trickle-infected with Ascaris and Trichuris: Heritabilities and associations with parasitological findings.
Vet Parasitol. 211 (3-4): 306-11. -
Kandasamy, S. et al. (2014) Prenatal vitamin A deficiency impairs adaptive immune responses to pentavalent rotavirus vaccine (RotaTeq®) in a neonatal gnotobiotic pig model.
Vaccine. 32 (7): 816-24. -
Kandasamy, S. et al. (2014) Lactobacilli and Bifidobacteria enhance mucosal B cell responses and differentially modulate systemic antibody responses to an oral human rotavirus vaccine in a neonatal gnotobiotic pig disease model.
Gut Microbes. 5 (5): 639-51. -
Makadiya, N. et al. (2016) S1 domain of the porcine epidemic diarrhea virus spike protein as a vaccine antigen.
Virol J. 13: 57. -
Nejsum P et al. (2009) Population dynamics of Ascaris suum in trickle-infected pigs.
J Parasitol. 95 (5): 1048-53.
Further Reading
-
Navarro, P. et al. (2000) Porcine IgA allotypes are not equally transcribed or expressed in heterozygous swine.
Mol Immunol. 37: 653-64.
