IgA antibody | K61 1B4

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Mouse anti Pig IgA

Product Type
Monoclonal Antibody
Clone
K61 1B4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA638GA C E F 0.1 mg
Mouse Anti Pig IgA antibody, clone K61 1B4 recognizes porcine immunoglobulin (Ig) alpha chain. No cross-reactivity is observed with either porcine IgM or IgG.

It has been suggested that pigs possess two subclasses of IgA, referred to as either IgA1 and IgA2 similar to the human IgA subclasses. More recent research indicates that these are allotypic variants and described as IgAa and IgAb (Navarro et al. 2000). Research undertaken in connection with porcine IgA response to Foot and Mouth disease (Pacheco et al. 2010) has demonstrated that clone K61 1B4 recognises both IgAa and IgAb allotypes.

IgA is the dominant immunoglobulin found in surface secretions where its role is in the protection of body surfaces. High levels of IgA may be detected in tracheal secretions, saliva, intestinal fluid and urogenital tract secretions. The primary function of IgA is to prevent adherence of bacteria and virus to epithelial surfaces. IgA may also act within epithelial cells where it has a role in interrupting viral replication.

Product Details

Target Species
Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Porcine IgA
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of IgA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/50K 1/500K
Flow Cytometry
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
ELISA
This product may be used as a detection reagent in ELISA applications

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Pig Ig Lambda Light Chain MCA633GA 0.1 mg C E F P
Mouse anti Pig IgA Secretory Component MCA634GA 0.1 mg E
Mouse anti Pig IgG1 MCA635GA 0.1 mg E
Mouse anti Pig IgG2 MCA636GA 0.1 mg E
Mouse anti Pig IgM MCA637GA 0.1 mg C E F

Application Based External Images

ELISA

Product Specific References

References for IgA antibody

  1. Leitão, A. et al. (2001) The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response.
    J Gen Virol. 82 (Pt 3): 513-23.
  2. Bourges, D. et al. (2004) T and IgA B lymphocytes of the pharyngeal and palatine tonsils: differential expression of adhesion molecules and chemokines.
    Scand J Immunol. 60 (4): 338-50.
  3. Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
    Parasitology. 136 (6): 691-7.
  4. Pacheco, J.M. et al. (2010) IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination.
    Clin Vaccine Immunol. 17: 550-8.
  5. Pasternak, J.A. et al. (2015) Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
    BMC Vet Res. 11 (1): 350.
  6. Ewaschuk JB et al. (2012) Barley-derived β-glucans increases gut permeability, ex vivo epithelial cell binding to E. coli, and naive T-cell proportions in weanling pigs.
    J Anim Sci. 90 (8): 2652-62.
  7. Inman CF et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
    PLoS One. 7 (3): e33707.
  8. Guzman-Bautista, E.R. et al. (2015) Tracheal and bronchial polymeric immunoglobulin secretory immune system (PISIS) development in a porcine model.
    Dev Comp Immunol. 53 (2): 271-82.
  9. Kringel H et al. (2015) Serum antibody responses in pigs trickle-infected with Ascaris and Trichuris: Heritabilities and associations with parasitological findings.
    Vet Parasitol. 211 (3-4): 306-11.
  10. Kandasamy, S. et al. (2014) Prenatal vitamin A deficiency impairs adaptive immune responses to pentavalent rotavirus vaccine (RotaTeq®) in a neonatal gnotobiotic pig model.
    Vaccine. 32 (7): 816-24.
  11. Kandasamy, S. et al. (2014) Lactobacilli and Bifidobacteria enhance mucosal B cell responses and differentially modulate systemic antibody responses to an oral human rotavirus vaccine in a neonatal gnotobiotic pig disease model.
    Gut Microbes. 5 (5): 639-51.
  12. Makadiya, N. et al. (2016) S1 domain of the porcine epidemic diarrhea virus spike protein as a vaccine antigen.
    Virol J. 13: 57.
  13. Nejsum P et al. (2009) Population dynamics of Ascaris suum in trickle-infected pigs.
    J Parasitol. 95 (5): 1048-53.

Further Reading

  1. Navarro, P. et al. (2000) Porcine IgA allotypes are not equally transcribed or expressed in heterozygous swine.
    Mol Immunol. 37: 653-64.