IgA antibody | K61 1B4



It has been suggested that pigs possess two subclasses of IgA, referred to as either IgA1 and IgA2 similar to the human IgA subclasses. More recent research indicates that these are allotypic variants and described as IgAa and IgAb (Navarro et al. 2000). Research undertaken in connection with porcine IgA response to Foot and Mouth disease (Pacheco et al. 2010) has demonstrated that clone K61 1B4 recognises both IgAa and IgAb allotypes.
IgA is the dominant immunoglobulin found in surface secretions where its role is in the protection of body surfaces. High levels of IgA may be detected in tracheal secretions, saliva, intestinal fluid and urogenital tract secretions. The primary function of IgA is to prevent adherence of bacteria and virus to epithelial surfaces. IgA may also act within epithelial cells where it has a role in interrupting viral replication.
Product Details
- Target Species
- Pig
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Porcine IgA
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of IgA antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/50K | 1/500K | |
Flow Cytometry | |||
Immunohistology - Frozen |
- ELISA
- This product may be used as a detection reagent in ELISA applications
Secondary Antibodies Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) | STAR117P | E WB | 0.5 mg |
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Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse anti Pig Ig Lambda Light Chain | MCA633GA | C E F P | 0.1 mg |
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|
Mouse anti Pig IgA Secretory Component | MCA634GA | E | 0.1 mg |
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|
Mouse anti Pig IgG1 | MCA635GA | E | 0.1 mg |
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|
Mouse anti Pig IgG2 | MCA636GA | E | 0.1 mg |
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|
Mouse anti Pig IgM | MCA637GA | C E F | 0.1 mg |
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Product Specific References
References for IgA antibody
-
Leitão, A. et al. (2001) The non-haemadsorbing African swine fever virus isolate ASFV/NH/P68 provides a model for defining the protective anti-virus immune response.
J Gen Virol. 82 (Pt 3): 513-23. -
Bourges, D. et al. (2004) T and IgA B lymphocytes of the pharyngeal and palatine tonsils: differential expression of adhesion molecules and chemokines.
Scand J Immunol. 60 (4): 338-50. -
Nejsum, P. et al. (2009) Population dynamics of Trichuris suis in trickle-infected pigs.
Parasitology. 136 (6): 691-7. -
Pacheco, J.M. et al. (2010) IgA antibody response of swine to foot-and-mouth disease virus infection and vaccination.
Clin Vaccine Immunol. 17: 550-8. -
Pasternak, J.A. et al. (2015) Oral antigen exposure in newborn piglets circumvents induction of oral tolerance in response to intraperitoneal vaccination in later life.
BMC Vet Res. 11 (1): 350. -
Ewaschuk JB et al. (2012) Barley-derived β-glucans increases gut permeability, ex vivo epithelial cell binding to E. coli, and naive T-cell proportions in weanling pigs.
J Anim Sci. 90 (8): 2652-62. -
Inman CF et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
PLoS One. 7 (3): e33707. -
Guzman-Bautista, E.R. et al. (2015) Tracheal and bronchial polymeric immunoglobulin secretory immune system (PISIS) development in a porcine model.
Dev Comp Immunol. 53 (2): 271-82. -
Kringel H et al. (2015) Serum antibody responses in pigs trickle-infected with Ascaris and Trichuris: Heritabilities and associations with parasitological findings.
Vet Parasitol. 211 (3-4): 306-11. -
Kandasamy, S. et al. (2014) Prenatal vitamin A deficiency impairs adaptive immune responses to pentavalent rotavirus vaccine (RotaTeq®) in a neonatal gnotobiotic pig model.
Vaccine. 32 (7): 816-24. -
Kandasamy, S. et al. (2014) Lactobacilli and Bifidobacteria enhance mucosal B cell responses and differentially modulate systemic antibody responses to an oral human rotavirus vaccine in a neonatal gnotobiotic pig disease model.
Gut Microbes. 5 (5): 639-51. -
Makadiya, N. et al. (2016) S1 domain of the porcine epidemic diarrhea virus spike protein as a vaccine antigen.
Virol J. 13: 57. -
Nejsum P et al. (2009) Population dynamics of Ascaris suum in trickle-infected pigs.
J Parasitol. 95 (5): 1048-53.
Further Reading
-
Navarro, P. et al. (2000) Porcine IgA allotypes are not equally transcribed or expressed in heterozygous swine.
Mol Immunol. 37: 653-64.
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