CD8 Alpha antibody | 11/295/33
Mouse anti Pig CD8 Alpha:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 11/295/33
- Isotype
- IgG2a
- Specificity
- CD8 Alpha
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD8 alpha antibody, clone 11/295/33 recognizes an epitope on the alpha chain of porcine CD8. Clone 11/295/33 detects a different epitope from that bound by clone 76-2-11. In addition to thymocytes and cytotoxic T cells, CD8 alpha is expressed on porcine NK cells. Swine CD8 alpha+CD3- NK cells are divided into NKp46+ (CD335) and NKp46- cells. A further CD8 alpha dim/- NKp46 high NK cell population can be found in porcine spleen and liver (Mair et al. 2012, 2013). Pigs are also remarkable in having significant levels of peripheral CD4 CD8 double positive T cells (Saalmüller et al. 1987; Zuckermann 1999). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- Porcine lymphocytes
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:RPE | MCA929PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse IgG2a Negative Control:RPE | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Pig CD8 Beta Chain:FITC | MCA5954F | F | 0.1 mg |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Pig CD8 Beta Chain:FITC | ||||||
| Mouse anti Pig CD4:Alexa Fluor® 647 | MCA6045A647 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse anti Pig CD4:Alexa Fluor® 647 | ||||||
References for CD8 Alpha antibody
-
Melgoza-González, A.E. et al. (2022) Antigen Targeting of Porcine Skin DEC205+ Dendritic Cells
Vaccines. 10 (5): 684.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
MCA6048PE
162081
1804
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD8 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Pig ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up