CD282 antibody | 1H11
Mouse anti Pig CD282:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 1H11
- Isotype
- IgG1
- Specificity
- CD282
Antibody Quality and Performance Guarantee
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| Mouse anti Pig CD282, clone 1H11 recognizes CD282, also known as toll-like receptor 2 (TLR2). Toll like receptors are a family of pattern-recognition receptors involved in the activation and regulation of inflammatory and immune responses activated by invasion of host tissues by microbial pathogens. TLR2 plays a role in a range of infections including gram-positive bacteria, mycobacteria, and yeast. It is activated by PAMPs, signals to NF-kappa-B which in turn induces cytokine secretion and the inflammatory response. Clone 1H11 was used to characterize the expression of porcine TLR2; cells of the innate immune system - monocytes, macrophages, and granulocytes, but not on peripheral blood lymphocytes were found to express porcine TLR2. Swine neutrophils express low levels of TLR2. It is also expressed on epithelial cells lining the tracheobronchial and intestinal tracts, bile ducts in the liver and renal tubules, and on the basal layer of the epidermis (Alvarez et al 2008). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Immunogen
- NIH/3T3 mouse cells transfected with plasmid pTLR2-GFP, encoding the porcine TLR2 tagged with the green fluorescent protein (GFP) on the carboxy-terminal end
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/2 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | Mouse IgG1 Negative Control:RPE | ||||||
Source Reference
-
Alvarez, B. et al. (2008) Expression of toll-like receptor 2 (TLR2) in porcine leukocyte subsets and tissues.
Vet Res. 39 (2): 13.
References for CD282 antibody
-
Álvarez-Estrada, Á. et al. (2019) TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis.
Comp Immunol Microbiol Infect Dis. 64: 31-39. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16): 2103.
Further Reading
-
Alvarez, B. et al. (2013) Antigen targeting to APC: from mice to veterinary species.
Dev Comp Immunol. 41 (2): 153-63.
- UniProt
- Q59HI8
MCA6165PE
163297
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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