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CD11c antibody | 3A8

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Mouse anti Pig CD11c:RPE

Product Type
Monoclonal Antibody
Clone
3A8
Isotype
IgG1
Specificity
CD11c

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Product Code Applications Pack Size List Price Your Price Qty
MCA6150PE
Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
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Anti Pig CD11c Antibody, clone 3A8 thumbnail image 1
Anti Pig CD11c Antibody, clone 3A8 gallery image 1
Figure A. FITC conjugated Mouse anti Pig CD14 (MCA1218F) and Mouse IgG1 Negative Control antibody (MCA928) detected with RPE conjugated Goat anti Mouse IgG1 antibody (STAR132PE). Figure B. FITC conjugated Mouse anti Pig CD14 (MCA1218F) and Mouse anti Pig CD11c (MCA6150GA) detected with RPE conjugated Goat anti Mouse IgG1 antibody (STAR132PE). All experiments performed on red cell lysed porcine blood gated on live, single mononuclear cells in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.

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FLA

Mouse anti Pig CD11c, clone 3A8 recognizes CD11c also known as integrin alpha-X.

Porcine CD11c is mainly expressed by blood and skin polymorphonuclear and mononuclear phagocytes as well as monocytes, granulocytes and alveolar and inflammatory macrophages. Porcine CD11c is not expressed by plasmacytoid dendritic cells (pDC).

Mouse anti Pig CD11c, clone 3A8 antibody can be used to distinguish resident from migrating dendritic cells (DC) in lymph nodes;migrated DC express lower levels of surface CD11c and have higher MHC class II expression than resident DC. Both porcine cDC1 and cDC2 subsets express CD11c, with cDC1 appearing to have slightly lower levels than cDC2. Clone 3A8 did not show species cross-reactivity with human CD11c-CD18 transfectants or sheep myeloid cells (Deloizy et al. 2016).

Target Species
Pig
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
Pig alveolar macrophages
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
RPE 561nm laser 546 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature.
Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests
List Price Your Price
Description Mouse IgG1 Negative Control:RPE

Antibody Characterization Reference

  1. Deloizy, C. et al. (2016) Expanding the tools for identifying mononuclear phagocyte subsets in swine: Reagents to porcine CD11c and XCR1.
    Dev Comp Immunol. 65: 31-40.

References for CD11c antibody

  1. Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
    Cells. 12 (16) :2103.

Synonyms
CD11
UniProt
K9IVW2

MCA6150PE

PDF 159407

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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