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CD11a antibody | BL1H8

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Anti Pig CD11a Antibody, clone BL1H8 thumbnail image 1 Anti Pig CD11a Antibody, clone BL1H8 thumbnail image 2
Anti Pig CD11a Antibody, clone BL1H8 gallery image 1
Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD11R1 (MCA1220) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD11a Antibody, clone BL1H8 gallery image 2
Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD46 (MCA2262) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse anti Pig CD11a:FITC

Product Type
Monoclonal Antibody
Clone
BL1H8
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA2308F
Datasheet
F
MSDS
0.1 mg loader

Mouse anti Pig CD11a, clone BL1H8 recognizes porcine CD11a, binding to a conformational epitope on the alpha chain of LFA-1 (CD11a/CD18), expressed on a range of cells including peripheral blood lymphocytes, monocytes, granulocytes and alveolar macrophages. Clone BL1H8 immunoprecipitates two bands of 170 kDa (CD11a) and 95 kDa (CD18) from porcine alveolar lysates, pre-clearance of the lysates completely removed the 170 kDa band , strongly suggesting the epitope for this antibody is located on the CD11a chain, although some involvement of residues on the LFA-1 β chain could not be formally ruled out (Alvarez et al. 2000 ). Mouse anti Pig CD11a, clone BL1H8 was clustered as porcine CD11a at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001).

Mouse anti pig CD11a, clone BL1H8 is reported to inhibit Con-A driven T-cell proliferation and the mixed lymphocyte reaction (Alvarez et al. 2000 ).

Product Details

Target Species
Pig
Species Cross-Reactivity
Target SpeciesCross Reactivity
Sheep
Bovine
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Porcine alveolar macrophages.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of CD11a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2b Negative Control:FITC MCA691F F 100 Tests loader

Application Based External Images

Flow Cytometry

Product Specific References

References for CD11a antibody

  1. Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.
    Xenotransplantation. 7 (4): 258-66.
  2. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
    J Immunol. 162 (9): 5230-7.
  3. Van de Walle, G.R. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells.
    J Gen Virol. 84 (Pt 3): 629-37.
  4. Vanden Bergh, P.G. et al. (2009) Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity.
    Vet Res. 40: 1-10.
  5. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation
    J Immunol. 162: 5230-7.
  6. Kyrova K et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
    BMC Vet Res. 10: 244.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
  2. Gerner W et al. (2015) Phenotypic and functional differentiation of porcine αβ T cells: current knowledge and available tools.
    Mol Immunol. 66 (1): 3-13.
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