CD11a antibody | BL1H8

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Anti Pig CD11a Antibody, clone BL1H8 thumbnail image 1

Anti Pig CD11a Antibody, clone BL1H8 thumbnail image 2

Anti Pig CD11a Antibody, clone BL1H8 gallery image 1 — Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD11R1 (MCA1220) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Anti Pig CD11a Antibody, clone BL1H8 gallery image 2 — Figure A. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1 PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD11a (MCA2308F) and purified mouse anti porcine CD46 (MCA2262) detected with goat anti mouse IgG1 (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.

Mouse anti Pig CD11a:FITC

Product Type: Monoclonal Antibody
Clone: BL1H8
Isotype: IgG2b

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2308F	F			0.1 mg

Mouse anti Pig CD11a, clone BL1H8 recognizes porcine CD11a, binding to a conformational epitope on the alpha chain of LFA-1 (CD11a/CD18), expressed on a range of cells including peripheral blood lymphocytes, monocytes, granulocytes and alveolar macrophages. Clone BL1H8 immunoprecipitates two bands of 170 kDa (CD11a) and 95 kDa (CD18) from porcine alveolar lysates, pre-clearance of the lysates completely removed the 170 kDa band , strongly suggesting the epitope for this antibody is located on the CD11a chain, although some involvement of residues on the LFA-1 β chain could not be formally ruled out (Alvarez et al. 2000 ). Mouse anti Pig CD11a, clone BL1H8 was clustered as porcine CD11a at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001).

Mouse anti pig CD11a, clone BL1H8 is reported to inhibit Con-A driven T-cell proliferation and the mixed lymphocyte reaction (Alvarez et al. 2000 ).

Product Details

Target Species

Pig

Species Cross-Reactivity

Target Species	Cross Reactivity
Sheep
Bovine

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%	Sodium Azide
1%	Bovine Serum Albumin

Immunogen

Porcine alveolar macrophages.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

Regulatory: For research purposes only

Applications of CD11a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry			Neat

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10⁶ cells in 100ul.

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG2b Negative Control:FITC	MCA691F	100 Tests	F

Application Based External Images

Flow Cytometry

Product Specific References

References for CD11a antibody

Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.
Xenotransplantation. 7 (4): 258-66.
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162 (9): 5230-7.
Van de Walle, G.R. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells.
J Gen Virol. 84 (Pt 3): 629-37.
Vanden Bergh, P.G. et al. (2009) Porcine CD18 mediates Actinobacillus pleuropneumoniae ApxIII species-specific toxicity.
Vet Res. 40: 1-10.
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation
J Immunol. 162: 5230-7.
Kyrova K et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
BMC Vet Res. 10: 244.