B Cell Subset antibody | BB6-10A10
Mouse anti Pig B Cell Subset:RPE
- Product Type
- Monoclonal Antibody
- Clone
- BB6-10A10
- Isotype
- IgM
- Specificity
- B Cell Subset
Antibody Quality and Performance Guarantee
Filter by Application:
F Reset| Mouse anti Pig B cell subset antibody, clone BB6-10A10, appears to recognize an immature pig B cell population. Clone BB6-10A10 stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood, 22-23% in mesenteric lymph nodes, 79-87% in ileal Peyer’s patches, 6-17 in spleen, and 0% in thymus (Boersma et al. 2001). |
- Target Species
- Pig
- Product Form
- Purified IgM conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgM prepared by combination of precipitation and chromatography techniques
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
Stabilizing agent (sucrose) - Approx. Protein Concentrations
- IgM concentration 0.1 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) RPE 488nm laser 496 578 RPE 561nm laser 546 578 - Regulatory
- For research purposes only
- Guarantee
- Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.
This product is shipped at ambient temperature.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti Pig CD45RA:FITC | MCA1751F | F | 0.1 mg |
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| List Price | Your Price | ||||||
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| Description | Mouse anti Pig CD45RA:FITC | ||||||
References for B Cell Subset antibody
-
Boersma, W.J. et al. (2001) Summary of workshop findings for porcine B-cell markers.
Vet Immunol Immunopathol. 80 (1-2): 63-78.
View more products with B Cell Subset specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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