TIM-2 antibody | RMT2-25
Filter by Application:
F ResetRat anti Mouse TIM-2
- Product Type
- Monoclonal Antibody
- Clone
- RMT2-25
- Isotype
- IgG2a
- Specificity
- TIM-2
Antibody Quality and Performance Guarantee
| Rat anti Mouse TIM-2 antibody, clone RMT2-25 recognizes mouse Tim-2 (T-cell immunoglobulin mucin 2), a transmembrane glycoprotein and member of the immunoglobulin superfamily, expressed by activated lymphocytes, developmentally in the 8-cell embryo and blastocyst, and preferentially up-regulated in Th2 cells and down-regulated in Th1 cells. Tim-2 acts as a negative regulator of Th2 cells, and is implicated in the development of atopic disease and other Th2-biased immune responses. Tim-2 also acts as a receptor for Semaphorin 4A, a transmembrane protein expressed on dendritic cells and B cells, which plays a role in the guidance of axonal migration during neuronal development, and has been shown to enhance T cell activation (Suzuki et al. 2007). Signaling by Tim-2 influences the proliferation and antibody production of B cells, during early phase collagen-induced arthritis and Rat anti Mouse TIM-2 antibody, clone RMT2-25 enhances the proliferation of stimulated splenic B cells in vitro (Kawamoto et al. 2011). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Tim-2-Ig, consisting of amino acids 1-230 of mouse Tim-2, coupled with the Fc portion of mouse IgG2a.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Lymph node cells from immunized Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
| Immunoprecipitation | |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Western Blotting
- Clone RMT2-25 recognises a band of approximately 55kDa in Tim-2/L5178Y cells under non-reducing conditions.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Rat IgG2a Negative Control | ||||||
References for TIM-2 antibody
-
Kawamoto, T. et al. (2011) Anti-T cell immunoglobulin and mucin domain-2 monoclonal antibody exacerbates collagen-induced arthritis by stimulating B cells.
Arthritis Res. Ther. 13: R47
Further Reading
-
Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
Immunol Rev. 235 (1): 172-89.
- Synonyms
- T Cell Immunoglobulin Mucin 2
- UniProt
- Q8R183
- Entrez Gene
- Timd2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
MCA5789GA
0811
163123
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Request a different product with this specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Mouse ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up