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TIM-2 antibody | RMT2-25

Rat anti Mouse TIM-2:FITC

Product Type
Monoclonal Antibody
Clone
RMT2-25
Isotype
IgG2a
Specificity
TIM-2

Product Code Applications Pack Size List Price Your Price Qty
MCA5789F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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loader

Rat anti Mouse TIM-2 antibody, clone RMT2-25 recognizes mouse Tim-2 (T-cell immunoglobulin mucin 2), a transmembrane glycoprotein and member of the immunoglobulin superfamily, expressed by activated lymphocytes, developmentally in the 8-cell embryo and blastocyst, and preferentially up-regulated in Th2 cells and down-regulated in Th1 cells.

Tim-2 acts as a negative regulator of Th2 cells, and is implicated in the development of atopic disease and other Th2-biased immune responses. Tim-2 also acts as a receptor for Semaphorin 4A, a transmembrane protein expressed on dendritic cells and B cells, which plays a role in the guidance of axonal migration during neuronal development, and has been shown to enhance T cell activation (Suzuki et al. 2007).

Signaling by Tim-2 influences the proliferation and antibody production of B cells, during early phase collagen-induced arthritis and Rat anti Mouse TIM-2 antibody, clone RMT2-25 enhances the proliferation of stimulated splenic B cells in vitro (Kawamoto et al. 2011).

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Tim-2-Ig, consisting of amino acids 1-230 of mouse Tim-2, coupled with the Fc portion of mouse IgG2a.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Lymph node cells from immunized Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A or BUF041B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
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List Price Your Price
loader
Description Rat IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for TIM-2 antibody

  1. Kawamoto, T. et al. (2011) Anti-T cell immunoglobulin and mucin domain-2 monoclonal antibody exacerbates collagen-induced arthritis by stimulating B cells.
    Arthritis Res. Ther. 13: R47

Further Reading

  1. Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
    Immunol Rev. 235 (1): 172-89.

Synonyms
T Cell Immunoglobulin Mucin 2
UniProt
Q8R183
Entrez Gene
Timd2
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity

MCA5789F

0811 0811R 163122

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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