TIM-2 antibody | RMT2-25
Rat anti Mouse TIM-2:FITC
- Product Type
- Monoclonal Antibody
- Clone
- RMT2-25
- Isotype
- IgG2a
- Specificity
- TIM-2
Rat anti Mouse TIM-2 antibody, clone RMT2-25 recognizes mouse Tim-2 (T-cell immunoglobulin mucin 2), a transmembrane glycoprotein and member of the immunoglobulin superfamily, expressed by activated lymphocytes, developmentally in the 8-cell embryo and blastocyst, and preferentially up-regulated in Th2 cells and down-regulated in Th1 cells. Tim-2 acts as a negative regulator of Th2 cells, and is implicated in the development of atopic disease and other Th2-biased immune responses. Tim-2 also acts as a receptor for Semaphorin 4A, a transmembrane protein expressed on dendritic cells and B cells, which plays a role in the guidance of axonal migration during neuronal development, and has been shown to enhance T cell activation (Suzuki et al. 2007). Signaling by Tim-2 influences the proliferation and antibody production of B cells, during early phase collagen-induced arthritis and Rat anti Mouse TIM-2 antibody, clone RMT2-25 enhances the proliferation of stimulated splenic B cells in vitro (Kawamoto et al. 2011). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Tim-2-Ig, consisting of amino acids 1-230 of mouse Tim-2, coupled with the Fc portion of mouse IgG2a.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Lymph node cells from immunized Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
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Rat IgG2a Negative Control:FITC | MCA1212F | F | 100 Tests | Log in | |||
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Description | Rat IgG2a Negative Control:FITC |
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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Mouse Seroblock FcR | BUF041A | F | 0.1 mg | Log in | |||
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Description | Mouse Seroblock FcR | ||||||
Mouse Seroblock FcR | BUF041B | F | 0.5 mg | Log in | |||
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Description | Mouse Seroblock FcR |
References for TIM-2 antibody
-
Kawamoto, T. et al. (2011) Anti-T cell immunoglobulin and mucin domain-2 monoclonal antibody exacerbates collagen-induced arthritis by stimulating B cells.
Arthritis Res. Ther. 13: R47
Further Reading
-
Freeman, G.J. et al. (2010) TIM genes: a family of cell surface phosphatidylserine receptors that regulate innate and adaptive immunity.
Immunol Rev. 235 (1): 172-89.
- Synonyms
- T Cell Immunoglobulin Mucin 2
- UniProt
- Q8R183
- Entrez Gene
- Timd2
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
MCA5789F
0811 0811R 163122If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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