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TCR Gamma/Delta antibody | GL3

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Hamster anti Mouse TCR Gamma/Delta:FITC

Product Type
Monoclonal Antibody
Clone
GL3
Isotype
IgG
Specificity
TCR Gamma/Delta

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Product Code Applications Pack Size List Price Your Price Qty
MCA1366F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 reacts with mouse TCR gamma/delta expressing lymphocytes.

Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 has been shown to have depleting activity in vivo (Van der Heyde et al. 1995 and  Skeenet al. 1993).

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
C57BL/6J intra epithelial lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Armenian hamster were fused with cells of the murine myeloma SP2/0 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1 x106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control:FITC MCA2356F F 0.1 mg loader
>
List Price Your Price
loader
Description Hamster (Armenian) IgG Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

References for TCR Gamma/Delta antibody

  1. Skeen, M.J. & Ziegler, H.K. (1993) Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection.
    J Exp Med. 178 (3): 971-84.
  2. Skarstein, K. et al. (1994) Oligoclonality of T cells in salivary glands of autoimmune MRL/lpr mice.
    Immunology. 81 (4): 497-501.
  3. van der Heyde, H.C. et al. (1995) Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria.
    J Immunol. 154 (8): 3985-90.
  4. Laky, K. et al. (2000) Enterocyte expression of interleukin 7 induces development of gammadelta T cells and Peyer's patches.
    J Exp Med. 191: 1569-80.
  5. Skelsey, M.E. et al. (2001) Gamma delta T cells are needed for ocular immune privilege and corneal graft survival.
    J Immunol. 166: 4327-33.
  6. Karasova, D. et al. (2010) Influence of 5 major Salmonella pathogenicity islands on NK cell depletion in mice infected with Salmonella enterica serovar Enteritidis.
    BMC Microbiol. 10: 75.

Flow Cytometry

Immunohistology - Frozen

RRID
AB_322442

MCA1366F

149770 155908 159980

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